TPRKB antibody
Principal name
TPRKB antibody
Alternative names for TPRKB antibody
TP53RK-binding protein, PRPK-binding protein, CGI-121, My019
Ncbi ID
9606, NP_057142, NP_057142.1, NM_016058, NP_001163959.1, BC027413, BC027162, NM_176842, NM_001170488
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Flow Cytometry (F), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of TPRKB antibody
- Kit Component:
- - KN318106G1, Tprkb gRNA vector 1 in pCas-Guide vector
- KN318106G2, Tprkb gRNA vector 2 in pCas-Guide vector
- KN318106D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Chambers,J.C., et al. (2010) Nat. Genet. 42 (5), 373-375
Western Blot analysis of TPRKB expression in transfected 293T cell line (H00051002-T01) by TPRKB MaxPab polyclonal antibody.Lane 1: CGI-121 transfected lysate(19.25 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of TPRKB expression in transfected 293T cell line (H00051002-T01) by TPRKB MaxPab polyclonal antibody.Lane 1: TPRKB transfected lysate(19.70 KDa).Lane 2: Non-transfected lysate.
Human THP-1; WB Suggested Anti-TPRKB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; TPRKB antibody - middle region (ARP56819_P050) in Human THP-1 cells using Western Blot
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800155)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800155)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TPRKB (RC206008, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPRKB.
HEK293T cells transfected with either RC206008 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TPRKB antibody(TA800155), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Anti-TPRKB mouse monoclonal antibody (TA800166) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TPRKB(RC206008).
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-TPRKB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC206008 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TPRKB antibody(TA800166), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TPRKB (RC206008, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPRKB.
Sample(30 ug whole cell lysate). A:293T. 12% SDS PAGE. TA308096 diluted at 1:500
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using TPRKB(TA308096) antibody at 1:250 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TPRKB(TA308096) antibody at 1:500 dilution.
WB Suggested Anti-TPRKB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: THP-1 cell lysate
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA800155 | TPRKB antibody |
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| TPRKB mouse monoclonal antibody, clone OTI3E3 (formerly 3E3) | |||||||
 |
Mouse | IgG2a | OTI3E3 | Purified | Hu | F, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
||||||
| TA800166 | TPRKB antibody |
||||||
| TPRKB mouse monoclonal antibody, clone OTI3H3 (formerly 3H3) | |||||||
 |
Mouse | IgG1 | OTI3H3 | Purified | Hu, Mky | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +16 additional images |
Highly validated antibody!
|
||||||
| TA800238 | TPRKB antibody |
||||||
| TPRKB mouse monoclonal antibody, clone OTI1E9 (formerly 1E9) | |||||||
 |
Mouse | IgG1 | OTI1E9 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +17 additional images |
Highly validated antibody!
|
||||||
| TA308096 | TPRKB antibody |
||||||
| Rabbit polyclonal antibody to TPRKB (TP53RK binding protein) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA345074 | TPRKB antibody |
||||||
| Rabbit Polyclonal Anti-TPRKB Antibody - middle region | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, GP, Hu, Ms, Por, Rt, Ze | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN318106 | Tprkb - mouse gene knockout kit via CRISPR |
||
 |
Mouse | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN318106BN | Tprkb - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN318106LP | Tprkb - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN318106RB | Tprkb - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP306008 | TPRKB |
||||
| Recombinant protein of human TP53RK binding protein (TPRKB) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
| AR50412PU-N | TPRKB (1-175, His-tag) |
||||
 |
Human | Purified | > 95 % by SDS - PAGE | E. coli |
0.25 mg /
€940.00
|
| Acris Antibodies GmbH | |||||
| AR50412PU-S | TPRKB (1-175, His-tag) |
||||
|
Human | Purified | > 95 % by SDS - PAGE | E. coli |
50 µg /
€370.00
|
| Acris Antibodies GmbH | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY414223 | TPRKB overexpression lysate |
|
|
0.1 mg /
€280.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC206008L2V | Lenti ORF particles, TPRKB (mGFP-tagged) - Human TP53RK binding protein (TPRKB), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€880.00
|
|
| OriGene Technologies, Inc. | ||
| RC206008L1V | Lenti ORF particles, TPRKB (Myc-DDK tagged) - Human TP53RK binding protein (TPRKB) , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€780.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201201L2V | Lenti ORF particles, Tprkb (GFP-tagged) - Mouse Tp53rk binding protein (cDNA clone MGC:36208 IMAGE:4022441), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€790.00
|
|
| OriGene Technologies, Inc. | ||
| MR201518L2V | Lenti ORF particles, Tprkb (GFP-tagged) - Mouse Tp53rk binding protein (cDNA clone MGC:37089 IMAGE:4951567), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€790.00
|
|
| OriGene Technologies, Inc. | ||
| MR216459L2V | Lenti ORF particles, Tprkb (GFP-tagged) - Mouse Tp53rk binding protein (Tprkb), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€880.00
|
|
| OriGene Technologies, Inc. | ||
| MR201525L2V | Lenti ORF particles, Tprkb (GFP-tagged) - Mouse Tp53rk binding protein (Tprkb), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€880.00
|
|
| OriGene Technologies, Inc. | ||
| MR201201L1V | Lenti ORF particles, Tprkb (Myc-DDK-tagged) - Mouse Tp53rk binding protein (cDNA clone MGC:36208 IMAGE:4022441), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€700.00
|
|
| OriGene Technologies, Inc. | ||
