TPRKB antibody
Principal name
TPRKB antibody
Alternative names for TPRKB antibody
TP53RK-binding protein, PRPK-binding protein, CGI-121, My019
Gene ID
51002 (TPRKB)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), EPair for Elisa (EPair), Flow Cytometry (F)
Background of TPRKB antibody
Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TPRKB acts as an allosteric effector that regulates the t(6)A activity of the complex. TPRKB is not required for tR modification.
General readings
Chambers,J.C., et al. (2010) Nat. Genet. 42 (5), 373-375
Western Blot analysis of TPRKB expression in transfected 293T cell line (H00051002-T01) by TPRKB MaxPab polyclonal antibody.Lane 1: CGI-121 transfected lysate(19.25 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of TPRKB expression in transfected 293T cell line (H00051002-T01) by TPRKB MaxPab polyclonal antibody.Lane 1: TPRKB transfected lysate(19.70 KDa).Lane 2: Non-transfected lysate.
Western Blot: TPRKB Lysate [NBL1-17228] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for TPRKB
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using TPRKB(GTX106781) antibody at 1:250 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TPRKB(GTX106781) antibody at 1:500 dilution.
Sample(30 ug whole cell lysate)A:293T12% SDS PAGEGTX106781 diluted at 1:500
Human heart tissue were subjected to SDS PAGE followed by western blot with 12800-1-AP(TPRKB antibody) at dilution of 1:300
Western Blot: TPRKB Antibody [NBP1-31618] - Western blot analysis of NBP1-31618 on 293T, with 12% SDS PAGE, diluted at 1:500.
Human THP-1; WB Suggested Anti-TPRKB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; TPRKB antibody - middle region (ARP56819_P050) in Human THP-1 cells using Western Blot
Western Blot: TPRKB Antibody [NBP1-56860] - This Anti-TPRKB antibody was used in Western Blot of THP-1 tissue lysate at a concentration of 1ug/ml.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800155)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800155)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TPRKB (RC206008, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPRKB.
HEK293T cells transfected with either RC206008 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TPRKB antibody(TA800155), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800166)
Anti-TPRKB mouse monoclonal antibody (TA800166) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TPRKB(RC206008).
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-TPRKB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC206008 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TPRKB antibody(TA800166), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800238)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TPRKB (RC206008, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPRKB.
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA800155 | TPRKB antibody |
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Mouse | IgG2a | OTI3E3 | Purified | Hu | F, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| TA800166 | TPRKB antibody |
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 |
Mouse | IgG1 | OTI3H3 | Purified | Hu, Mky | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +16 additional images |
Highly validated antibody!
|
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| TA800238 | TPRKB antibody |
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 |
Mouse | IgG1 | OTI1E9 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +17 additional images |
Highly validated antibody!
|
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| TA308096 | TPRKB antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA345074 | TPRKB antibody |
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 |
Rabbit | IgG | Purified | Bov, Can, GP, Hu, Ms, Por, Rt, Ze | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| H00051002-A01 | TPRKB antibody |
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 |
Mouse | Serum | Hu | E, WB |
50 µl /
€290.00
|
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| Abnova Taiwan Corp. | |||||||
| H00051002-B01P | TPRKB antibody |
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 |
Mouse | Purified | Hu | E(detection), WB |
50 µg /
€370.00
|
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| Abnova Taiwan Corp. | |||||||
| H00051002-D01P | TPRKB antibody |
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 |
Rabbit | Purified | Hu | E(detection), WB |
0.1 mg /
€380.00
|
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| Abnova Taiwan Corp. | |||||||
| H00051002-M01 | TPRKB antibody |
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|
Mouse | IgG1 | 3F6 | Purified | Hu | E, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| ARP56819_P050 | TPRKB antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
50 µg /
€350.00
|
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| AVIVA Systems Biology | |||||||
| NBP1-31618 | TPRKB antibody |
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![Western Blot: TPRKB Antibody [NBP1-31618] - Western blot analysis of NBP1-31618 on 293T, with 12% SDS PAGE, diluted at 1:500.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/110062_1.jpg) |
Rabbit | IgG | Hu | ICC/IF, P, WB |
0.1 mg /
€420.00
|
||
| Novus Biologicals Inc. | |||||||
| NBP1-56860 | TPRKB antibody |
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![Western Blot: TPRKB Antibody [NBP1-56860] - This Anti-TPRKB antibody was used in Western Blot of THP-1 tissue lysate at a concentration of 1ug/ml.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/TPRKB Antibody-Western Blot-NBP1-56860-img0001.jpg) |
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
50 µg /
€440.00
|
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| Novus Biologicals Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP306008 | TPRKB |
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 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
| AR50412PU-N | TPRKB (1-175, His-tag) |
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Human | Purified | > 95 % by SDS - PAGE | E. coli |
0.25 mg /
€940.00
|
| Acris Antibodies GmbH | |||||
| AR50412PU-S | TPRKB (1-175, His-tag) |
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|
Human | Purified | > 95 % by SDS - PAGE | E. coli |
50 µg /
€370.00
|
| Acris Antibodies GmbH | |||||
| H00051002-P01-10 | TPRKB |
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Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
10 µg /
€380.00
|
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| Abnova Taiwan Corp. | |||||
| H00051002-P01-25 | TPRKB |
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Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
25 µg /
€510.00
|
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| Abnova Taiwan Corp. | |||||
| H00051002-Q01-10 | TPRKB |
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|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
10 µg /
€380.00
|
|
| Abnova Taiwan Corp. | |||||
| H00051002-Q01-25 | TPRKB |
||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
25 µg /
€510.00
|
|
| Abnova Taiwan Corp. | |||||
Antibody Pairs
| Catalog No. | ||
|---|---|---|
| H00051002-AP21 | TPRKB (Human) Matched Antibody Pair |
|
|
1 set /
€710.00
|
|
| Abnova Taiwan Corp. |
