TPX2 / DIL2 antibody

Principal name

TPX2 / DIL2 antibody

Alternative names for TPX2 / DIL2 antibody

DIL-2, C20orf1, C20orf2, HCA519, Targeting protein for Xklp2, Restricted expression proliferation-associated protein 100, Differentially expressed in cancerous and non-cancerous lung cells 2, Hepatocellular carcinoma-associated antigen 519

SwissProt ID

Q9ULW0 (Human)

Gene ID

22974 (TPX2), 7122974 (flgH)

Available reactivities

Hu (Human), Mam (Mammalia), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Gt (Goat), Hst (Hamster), Sh (Sheep), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C)

Background of TPX2 / DIL2 antibody

The TPX2 protein, also known as targeting protein for Xklp1, restricted expression proliferation associated protein p100, and differentially expressed in cancer and non-cancerous lung cells protein 2 (DIL2), is a 100 kD nuclear protein that contains two coiled-coil domains. The TPX2 protein is strictly associated with spindle pole and mitotic spindle during mitosis. In the G2/S position of the cell cycle, TPX2 is diffusely distributed throughout nucleus. TPX2 has been shown to be highly expressed in lung carcinomas cell lines, but not in normal lung tissues. TPX2 is thought to be required for the Ran-GTP dependent assembly of microtubules around chromosomes required to generate stable bipolar spindle with overlapping anti-parallel microtubule arrays and may also be involved in targeting Aurora-A kinase to the mitotic spindle. TPX2 has been shown to interact with a large number of proteins including serine/threonine protein kinase 6, ribosomal protein 6, Bop-1, alpha-tubulin, and nucleolin among others. TPX2 can be modified by phosphorylation on serine 738. The 18D5 monoclonal antibody recognizes human TPX2 and has been shown to be useful for Western blotting.

General readings

Szczepanowski,M., (2007) Biochem. Biophys. Res. Commun. 362 (2), 485-490

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TPX2 / DIL2 antibody

Western Blot: TPX2 Antibody TA301667 - Analysis of TPX2 expression in 1) HeLa, 2) Ntera2, 3) K-562 and 4) Raji whole cell lysates using NB500-179. Rabbit IgG Purified Bov, Gt, Hst, Hu, Ms, Sh WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TPX2 / DIL2 antibody

Western blot analysis of extracts from Jurkat cells, using DIL-2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TPX2 / DIL2 (1-220) antibody

MOLT-4 nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-TPX2 (clone 18D5) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 18D5 Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TPX2 / DIL2 antibody

WB Suggested Anti-TPX2 Antibody Titration: 0.2-1 ug/ml; Positive Control: 721_B cell lysate.TPX2 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

TPX2 / DIL2 (1-220) antibody

MOLT-4 nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-TPX2 (clone 18D5) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 18D5 Aff - Purified Hu ICC/IF, P, WB
25 µg / €210.00
  Acris Antibodies GmbH

TPX2 / DIL2 antibody

Figure 1. Western analysis of extracts from Jurkat cells using P100 antibody (Cat. #AM11020PU-N). Mouse IgG1 19AT738.292 Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TPX2 / DIL2 antibody

Figure 1. Western analysis of extracts from Jurkat cells using P100 antibody (Cat. #AM11022PU-N). Mouse IgG1 19AT953.286.93 Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TPX2 / DIL2 antibody

Immunohistochemistry (IHC) analyzes of TPX2 antibody (Cat.-No.: AP01372PU-N) in paraffin-embedded human brain tissue Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TPX2 / DIL2 antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TPX2 / DIL2 antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TPX2 / DIL2 antibody

TPX2 / DIL2 Mouse IgM 5E11 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

TPX2 / DIL2 antibody

TPX2 / DIL2 Rabbit Hu IP, WB
0.1 ml / €400.00
  Abnova Taiwan Corp.

TPX2 / DIL2 antibody

Immunofluorescence staining of TPX2 on nuclears of HeLa cells fixed in 3.5% paraformaldehyde during interphase and spindle staining during mitosis with TPX2 polyclonal antibody at 1 : 1000 ( Cat # PAB11993 ). Rabbit Hu, Ms, Rt ICC/IF, IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

TPX2 / DIL2 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using DIL-2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €205.00
  Assay Biotech

TPX2 / DIL2 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using DIL-2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €310.00
  Assay Biotech

TPX2 / DIL2 antibody

Human 721_B; WB Suggested Anti-TPX2 Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysate. TPX2 is supported by BioGPS gene expression data to be expressed in 721_B.; TPX2 antibody - C-terminal region (ARP46176_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

TPX2 / DIL2 Super Novus Pack antibody

Western Blot and  Immunoprecipitation: TPX2 Antibodies SuperNovus Pack [NB100-940] - Detection of Human TPX2 on HeLa whole cell lysate using NB500-183. TPX2 was also immunoprecipitated using NB500-184 at 0.4 ug/mg lysate. Rabbit Aff - Purified Hu IP, WB
2 vials / €440.00
  Novus Biologicals Inc.

TPX2 / DIL2 antibody

Western Blot: TPX2 Antibody (18D5-1) [NB100-74556] - Western blot detection of TPX2 using NB100-74556. Mouse IgG 18D5-1 Purified Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

TPX2 / DIL2 antibody

MOLT-4 nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-TPX2 (clone 18D5) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 18D5 Purified Hu C, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

TPX2 / DIL2 antibody

TPX2 / DIL2 Rabbit Hu, Mam, Ms, Rt ICC/IF, IP, WB
25 µl / €200.00
  Novus Biologicals Inc.

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