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TRADD antibody

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Principal name

TRADD antibody

Alternative names for TRADD antibody

TNFR1-associated DEATH domain protein, TNFRSF1A-associated via death domain, Tumor necrosis factor receptor type 1-associated DEATH domain protein

SwissProt ID

Q15628 (Human), Q2KI74 (Bovin), Q3U0V2 (Mouse)

Gene ID

8717 (TRADD), 71609 (Tradd)

Ncbi ID

NP_003780.1, 9606, NP_003780, NM_003789, NP_001028333.1, NM_001033161

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Mky (Monkey), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of TRADD antibody

TRADD is the adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death sigling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.

AP00240PU-N - TRADD
Western blot analysis of TRADD expression in mouse small intestine.
AP06467PU-N - TRADD
Immunohistochemistry (IHC) analyzes of TRADD antibody (Cat.-No.: AP06467PU-N) in paraffin-embedded human breast carcinoma tissue.
AP06467PU-N - TRADD
Western blot (WB) analysis of TRADD antibody (Cat.-No.: AP06467PU-N) at 1/500 dilution
Lane1:HEK293T whole cell lysate
Lane2:Mouse heart tissue lysate
Lane3:Rat liver tissue lysate
H00008717-M01 - TRADD
TRADD monoclonal antibody (M01), clone 3G1. Western Blot analysis of TRADD expression in Hela NE ( Cat # L013V3 ).
AP15828PU-S - TRADD
Liver carcinoma stained with Anti-TRADD antibody
H00008717-D01P - TRADD
TRADD MaxPab rabbit polyclonal antibody. Western Blot analysis of TRADD expression in human pancreas.
H00008717-D01P - TRADD
Western Blot analysis of TRADD expression in transfected 293T cell line (H00008717-T01) by TRADD MaxPab polyclonal antibody.Lane 1: TRADD transfected lysate(34.20 KDa).Lane 2: Non-transfected lysate.
PAB9061 - TRADD
Western blot analysis of TRADD expression in mouse small intestine tissue lysate.
AVARP02012_P050 - TRADD
Human 293T; . Lanes: Lane 1: 10ug Tradd-HA-Strep-stable expression 293TREXFlpIn cells-Doxycycline induced. Lane 2: 10ug ITradd-HA-Strep-stable expression 293TREXFlpIn cells-non-induced. Lane 3: 10ug siRNA scrambled-MDA-MB-231 cells. Lane 4: siRNA Tradd-MDA-MB-231 cells. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:2000. Gene Name: TRADD. Submitted by: Dr. Tencho Tenev, The Breakthrough Breast Cancer Research Centre, Institute of Cancer Research. There is BioGPS gene expression data showing that TRADD is expressed in HEK293T.; TRADD antibody - middle region (AVARP02012_P050) in Human 293T cells using Western Blot
AVARP02012_P050 - TRADD
Human DU145; WB Suggested Anti-TRADD Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: DU145 cell lysate. There is BioGPS gene expression data showing that TRADD is expressed in DU145.; TRADD antibody - middle region (AVARP02012_P050) in Human DU145 cells using Western Blot
C0377-1 - TRADD
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TRADD Antibody. The picture on the right is treated with the synthesized peptide.
C0377-1 - TRADD
Western blot analysis of extracts from COS7 cells, using TRADD Antibody. The lane on the right is treated with the synthesized peptide.
AP54350PU-N - TRADD
TRADD Antibody (Center) (AP54350PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TRADD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
AP54350PU-N - TRADD
Confocal immunofluorescent analysis of TRADD Antibody (Center) (AP54350PU-N) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
AP54350PU-N - TRADD
TRADD Antibody (Center) (AP54350PU-N) western blot analysis in Hela cell line lysates (35 µg/lane). This demonstrates the TRADD antibody detected the TRADD protein (arrow).
AP54350PU-N - TRADD
TRADD Antibody (Center) (AP54350PU-N) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AP32245PU-N - TRADD
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP32245PU-N - TRADD
Western blot analysis of extracts from COS7 cells, using TRADD Antibody Cat.-No AP32245PU-N. The lane on the right is treated with the synthesized peptide.
CPA4296-100ul - TRADD
Immunohistochemical analysis of TRADD staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA4296-100ul - TRADD
Western blot analysis of TRADD expression in K562 (A); mouse heart (B); rat liver (C) whole cell lysates.
TA308445 - TRADD
Sample (10 ug of whole cell lysate). A: A549. B: SiHa. 10% SDS PAGE. TA308445 diluted at 1:1000.
TA308445 - TRADD
TRADD antibody [N1], N-term detects TRADD protein at cytosol on colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. TRADD antibody [N1], N-term (TA308445) dilution: 1:500.
TA308445 - TRADD
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using TRADD(TA308445) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.
TA312115 - TRADD
Western blot analysis of extracts from COS7 cells, using TRADD antibody.The lane on the right is treated with the synthesized peptide.
TA312115 - TRADD
Western blot analysis of extracts from COS7 cells (Lane 2) and HuvEc cells (Lane 3), using TRADD Antibody. The lane on the left is treated with systhesized peptide.
TA312115 - TRADD
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using TRADD antibody.The picture on the right is treated with the synthesized peptide.
TA326949 - TRADD
Western blot analysis of extracts from K562 cells, treated with EGF (40nM, 30mins), using eNOS (Phospho-Ser615) antibody.The lane on the right is treated with the synthesized peptide.
TA330326 - TRADD
WB Suggested Anti-TRADD Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: DU145 cell lysateThere is BioGPS gene expression data showing that TRADD is expressed in DU145
TA330326 - TRADD
Lanes: Lane 1: 10ug Tradd-HA-Strep-stable expression 293T REXFlpIn cells-Doxycycline induced; Lane 2: 10 ug ITradd-HA-Strep-stable expression 293T REXFlpIn cells-non-induced; Lane 3: 10 ug siRNA scrambled-MDA-MB-231 cells; Lane 4: siRNA Tradd-MDA-MB-231 c
TA347499 - TRADD
Western blot analysis on COS7 and HuvEc cell lysate using TRADD Antibody
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TRADD (Center) antibody
TRADD Antibody (Center) (AP54350PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TRADD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH
+3 additional images

Highly validated antibody!

TRADD antibody
  Rabbit Polyclonal antibody to TRADD (TNFRSF1A-associated via death domain)  
Sample (10 ug of whole cell lysate). A: A549. B: SiHa. 10% SDS PAGE. TA308445 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
+2 additional images

TRADD antibody
  Rabbit polyclonal anti-TRADD antibody  
Western blot analysis of extracts from COS7 cells, using TRADD antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
+2 additional images

TRADD antibody
  Rabbit anti-TRADD Polyclonal Antibody  
Western blot analysis of extracts from K562 cells, treated with EGF (40nM, 30mins), using eNOS (Phospho-Ser615) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRADD antibody
  Rabbit Polyclonal Anti-TRADD Antibody  
WB Suggested Anti-TRADD Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: DU145 cell lysateThere is BioGPS gene expression data showing that TRADD is expressed in DU145 Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.
+1 additional image

TRADD antibody
  Rabbit Polyclonal Anti-TRADD Antibody  
Western blot analysis on COS7 and HuvEc cell lysate using TRADD Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRADD control peptide
TRADD Purified
50 µg / €180.00
  Acris Antibodies GmbH

TRADD antibody
Western blot analysis of TRADD expression in mouse small intestine. Rabbit Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

TRADD antibody
Immunohistochemistry (IHC) analyzes of TRADD antibody (Cat.-No.: AP06467PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH
+1 additional image

TRADD (C-term) antibody
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH
+1 additional image

TRADD antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TRADD Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €230.00
  Assay Biotech
+1 additional image

TRADD antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TRADD Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €330.00
  Assay Biotech
+1 additional image

CRISPR

Catalog No. Species Donor Vector  

TRADD - human gene knockout kit via CRISPR
TRADD - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

TRADD - human gene knockout kit via CRISPR
TRADD - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

TRADD - human gene knockout kit via CRISPR
TRADD - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

TRADD - human gene knockout kit via CRISPR
TRADD - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Tradd - mouse gene knockout kit via CRISPR
Tradd - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Tradd - mouse gene knockout kit via CRISPR
Tradd - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Tradd - mouse gene knockout kit via CRISPR
Tradd - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Tradd - mouse gene knockout kit via CRISPR
Tradd - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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25 products - FILTER YOUR RESULTS!

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Target
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Reactivity
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  1. Acris - OriGene (23)
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Service
  • OriGene EU
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