TRADD antibody
Principal name
TRADD antibody
Alternative names for TRADD antibody
TNFR1-associated DEATH domain protein, TNFRSF1A-associated via death domain, Tumor necrosis factor receptor type 1-associated DEATH domain protein
Ncbi ID
NP_003780.1, 9606, NP_003780, NM_003789, NP_001028333.1, NM_001033161
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of TRADD antibody
TRADD is the adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death sigling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.
Western blot analysis of TRADD expression in mouse small intestine.
Immunohistochemistry (IHC) analyzes of TRADD antibody (Cat.-No.: AP06467PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of TRADD antibody (Cat.-No.: AP06467PU-N) at 1/500 dilution
Lane1:HEK293T whole cell lysate
Lane2:Mouse heart tissue lysate
Lane3:Rat liver tissue lysate
Lane1:HEK293T whole cell lysate
Lane2:Mouse heart tissue lysate
Lane3:Rat liver tissue lysate
TRADD monoclonal antibody (M01), clone 3G1. Western Blot analysis of TRADD expression in Hela NE ( Cat # L013V3 ).
Liver carcinoma stained with Anti-TRADD antibody
TRADD MaxPab rabbit polyclonal antibody. Western Blot analysis of TRADD expression in human pancreas.
Western Blot analysis of TRADD expression in transfected 293T cell line (H00008717-T01) by TRADD MaxPab polyclonal antibody.Lane 1: TRADD transfected lysate(34.20 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of TRADD expression in mouse small intestine tissue lysate.
Human 293T; . Lanes: Lane 1: 10ug Tradd-HA-Strep-stable expression 293TREXFlpIn cells-Doxycycline induced. Lane 2: 10ug ITradd-HA-Strep-stable expression 293TREXFlpIn cells-non-induced. Lane 3: 10ug siRNA scrambled-MDA-MB-231 cells. Lane 4: siRNA Tradd-MDA-MB-231 cells. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:2000. Gene Name: TRADD. Submitted by: Dr. Tencho Tenev, The Breakthrough Breast Cancer Research Centre, Institute of Cancer Research. There is BioGPS gene expression data showing that TRADD is expressed in HEK293T.; TRADD antibody - middle region (AVARP02012_P050) in Human 293T cells using Western Blot
Human DU145; WB Suggested Anti-TRADD Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: DU145 cell lysate. There is BioGPS gene expression data showing that TRADD is expressed in DU145.; TRADD antibody - middle region (AVARP02012_P050) in Human DU145 cells using Western Blot
TRADD Antibody (Center) (AP54350PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TRADD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of TRADD Antibody (Center) (AP54350PU-N) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
TRADD Antibody (Center) (AP54350PU-N) western blot analysis in Hela cell line lysates (35 µg/lane). This demonstrates the TRADD antibody detected the TRADD protein (arrow).
TRADD Antibody (Center) (AP54350PU-N) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of extracts from COS7 cells, using TRADD Antibody Cat.-No AP32245PU-N. The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of TRADD staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of TRADD expression in K562 (A); mouse heart (B); rat liver (C) whole cell lysates.
Sample (10 ug of whole cell lysate). A: A549. B: SiHa. 10% SDS PAGE. TA308445 diluted at 1:1000.
TRADD antibody [N1], N-term detects TRADD protein at cytosol on colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. TRADD antibody [N1], N-term (TA308445) dilution: 1:500.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using TRADD(TA308445) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.
Western blot analysis of extracts from COS7 cells, using TRADD antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from COS7 cells (Lane 2) and HuvEc cells (Lane 3), using TRADD Antibody. The lane on the left is treated with systhesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using TRADD antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from K562 cells, treated with EGF (40nM, 30mins), using eNOS (Phospho-Ser615) antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-TRADD Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: DU145 cell lysateThere is BioGPS gene expression data showing that TRADD is expressed in DU145
Lanes: Lane 1: 10ug Tradd-HA-Strep-stable expression 293T REXFlpIn cells-Doxycycline induced; Lane 2: 10 ug ITradd-HA-Strep-stable expression 293T REXFlpIn cells-non-induced; Lane 3: 10 ug siRNA scrambled-MDA-MB-231 cells; Lane 4: siRNA Tradd-MDA-MB-231 c
Western blot analysis on COS7 and HuvEc cell lysate using TRADD Antibody
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP54350PU-N | TRADD (Center) antibody |
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Rabbit | Ig | Aff - Purified | Hu | F, ICC/IF, P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA308445 | TRADD antibody |
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| Rabbit Polyclonal antibody to TRADD (TNFRSF1A-associated via death domain) | |||||||
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Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA312115 | TRADD antibody |
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| Rabbit polyclonal anti-TRADD antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA326949 | TRADD antibody |
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| Rabbit anti-TRADD Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA330326 | TRADD antibody |
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| Rabbit Polyclonal Anti-TRADD Antibody | |||||||
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Rabbit | IgG | Purified | Hu | WB |
0.1 ml /
€360.00
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|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA347499 | TRADD antibody |
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| Rabbit Polyclonal Anti-TRADD Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP06467PU-N | TRADD antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms | P, WB |
0.1 mg /
€370.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP32245PU-N | TRADD (C-term) antibody |
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Rabbit | IgG | Aff - Purified | Hu, Ms | E, P, WB |
50 µl /
€425.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY401247 | TRADD overexpression lysate |
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC202599L2V | Lenti ORF particles, TRADD (mGFP-tagged) - Human TNFRSF1A-associated via death domain (TRADD), 200 uL, >10^7 TU/mL |
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200 µl /
€1,020.00
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| OriGene Technologies, Inc. | ||
| RC202599L1V | Lenti ORF particles, TRADD (Myc-DDK tagged) - Human TNFRSF1A-associated via death domain (TRADD) , 200 uL, >10^7 TU/mL |
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200 µl /
€910.00
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|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR223646L2V | Lenti ORF particles, Tradd (GFP-tagged) - Mouse TNFRSF1A-associated via death domain (Tradd), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
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| OriGene Technologies, Inc. | ||
| MR223646L1V | Lenti ORF particles, Tradd (Myc-DDK-tagged) - Mouse TNFRSF1A-associated via death domain (Tradd), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
