TRAF2 / TRAP3 antibody

Principal name

TRAF2 / TRAP3 antibody

Alternative names for TRAF2 / TRAP3 antibody

TNF receptor-associated factor 2, Tumor necrosis factor type 2 receptor-associated protein 3

SwissProt ID

P39429 (Mouse), Q12933 (Human)

Gene ID

7186 (TRAF2), 22030 (Traf2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TRAF2 / TRAP3 antibody

TRAFs (TNF receptor-associated factors) are a family of multifunctiol adaptor proteins that bind to surface receptors and recruit additiol proteins to form multiprotein sigling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-termil "TRAF domain" which mediates interactions with associated proteins; many also contain amino-termil Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses. While TRAF2 was origilly described through its interaction with TNFRII, it has since been shown to interact with other surface receptors including CD27, CD30, CD40, 4-1BB, Ox40, HVEM/ATAR and LMP-1. TRAF2 also associates with a large number of intracellular proteins, including TRADD, FADD, I-TRAF/TANK, TRIP, A20, c-IAP1 and 2, Casper, RIP and NIK, which help to regulate cell survival. Domint negative and knockout studies have shown that TRAF2 plays an important role in TNF-mediated activation of NF-kB and the MAPK/JNK kise pathway.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TRAF2 / TRAP3 (C-term) antibody

  Rabbit Polyclonal Antibody against TRAF2 (C-term)  
Western blot analysis of TRAF2 (arrow) using rabbit polyclonal TRAF2 Antibody (C-term) (Cat.#TA302132). 293 cell lysates (2 ug/lane) either nontransfected (c) or transiently transfected with the TRAF2 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRAF2 / TRAP3 (C-term) antibody

  Goat Polyclonal Antibody against TRAF2  
TA302626 (0.1µg/ml) staining of human ovary lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRAF2 / TRAP3 (C-term) antibody

  Rabbit Polyclonal TRAF2 Antibody  
Western blot analysis of TRAF2 in mouse liver tissue lysate with TRAF2 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRAF2 / TRAP3 (N-term) antibody

  Rabbit Polyclonal TRAF2 Antibody  
Western blot analysis of TRAF2 in human liver tissue lysate with TRAF2 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRAF2 / TRAP3 antibody

  Rabbit polyclonal anti-TRAF2 antibody  
WB using Anti-TRAF2 antibody shows detection of endogenous TRAF2 in whole HeLa cell lysates. Lane 2 shows endogenous TRAF2 detected with antibody at 47 kDa (arrowhead). Lane 4 shows no reactivity when blot is incubated with immunizing peptide.  Briefly, each lane contains approximately 14 µg of lysate. Primary antibody was used at 1:500. Gt-a-Rabbit DyLight 649 was used at 1:20000. Rabbit IgG Aff - Purified Hu, Ms C, E, IP, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

TRAF2 / TRAP3 antibody

  Anti-TRAF2 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using TRAF2 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TRAF2 / TRAP3 antibody

  Mouse monoclonal TRAF2 Antibody  
TRAF2/MB10188 antibody (Cat. #TA324340) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the TRAF2/MB10188 antibody detected the TRAF2/MB10188 protein (arrow). Mouse IgG1 214CT16.3.4 Purified Hu ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

TRAF2 / TRAP3 antibody

  TRAF2 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using TRAF2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRAF2 / TRAP3 antibody

  Mouse Monoclonal TRAF-2 Antibody (33A1293)  
Western Blot: TRAF2 Antibody (33A1293) [TA336458] - analysis of TRAF2 in HeLa lysate using TRAF2 antibody at 2 ug/ml. Mouse IgG1 33A1293 Purified Hu C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRAF2 / TRAP3 antibody

Small intestine: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

TRAF2 - human gene knockout kit via CRISPR

TRAF2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

TRAF2 - human gene knockout kit via CRISPR

TRAF2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

TRAF2 - human gene knockout kit via CRISPR

TRAF2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

TRAF2 - human gene knockout kit via CRISPR

TRAF2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Traf2 - mouse gene knockout kit via CRISPR

Traf2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Traf2 - mouse gene knockout kit via CRISPR

Traf2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Traf2 - mouse gene knockout kit via CRISPR

Traf2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Traf2 - mouse gene knockout kit via CRISPR

Traf2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TRAF2 / TRAP3

  Recombinant protein of human TNF receptor-associated factor 2 (TRAF2)  
TRAF2 / TRAP3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, TRAF2 (mGFP-tagged) - Human TNF receptor-associated factor 2 (TRAF2), 200 uL, >10^7 TU/mL

Lenti ORF particles, TRAF2 (mGFP-tagged) - Human TNF receptor-associated factor 2 (TRAF2), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

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