TRAF3 / CRAF1 antibody

Principal name

TRAF3 / CRAF1 antibody

Alternative names for TRAF3 / CRAF1 antibody

TNF receptor-associated factor 3, CAP1, CAP-1, LMP1-associated protein, LAP1, CD40-binding protein, CD40BP, CD40 receptor-associated factor 1

SwissProt ID

Q13114 (Human), Q60803 (Mouse)

Gene ID

7187 (TRAF3), 22031 (Traf3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP)

Background of TRAF3 / CRAF1 antibody

Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the Interleukin 1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6). TRAF3, originally named CRAF1, interacts directly with the CD40 cytoplasmic tail through a region of similarity to the tumor necrosis factor-alpha (TNF-alpha) receptor-associated factors. TRAF3 binds only a single site, which contains the sequence PEQET, whereas TRAF1 and TRAF2 are capable of binding to either the PEQET site or an additional downstream domain.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TRAF3 / CRAF1 (C-term) antibody

Western blot analysis of TRAF3 in 3T3 cell lysate with TRAF3 antibody at (A) 1, and (B) 2 µg/ml. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRAF3 / CRAF1 (N-term) antibody

Western blot analysis of TRAF3 in HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRAF3 / CRAF1 antibody

Predicted band size: 64kDa. Positive control: Human fetal kidney tissue lysate. Recommended dilution: 1/200-1000. (Gel: 8+10+12%SDS-PAGE Lysate: 60 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TRAF3 / CRAF1 antibody

Predicted band size: 64 kDa . Positive control: Mouse kidney tissue lysate. Recommended dilution: 1/200-1000. (Gel: %SDS-PAGE Lane1: Mouse kidney tissue lysate Lysate: 60 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TRAF3 / CRAF1 antibody

Western Blot: TRAF3 Antibody [TA336438] - Analysis of TRAF3 in (A) HeLa and (B) NIH 3T3 cell lysate using the TRAF3 antibody at 2 ug/ml. Rabbit Purified Hu, Ms P, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

TRAF3 / CRAF1 antibody

Western blot analysis of TRAF3 expression in NIH-3T3 cells Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRAF3 / CRAF1 antibody

Western blot analsis of TRAF3 in lysates from Jurkat cells (Lane 1+2), 3T3 cells (Lane 3) and Rat kidney (Lane 4). Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

TRAF3 / CRAF1 antibody

Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TRAF3 / CRAF1 antibody

Figure 1. Staining TRAF3 in Spleen by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Aff - Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TRAF3 / CRAF1 antibody

Western blot analysis of TRAF3 Antibody Cat.-No AP20591PU-N at 1/500 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: HEK293T whole cell lysate. Lane 3: NIH-3T3 whole cell lysate. Lane 4: Mouse lung tissue lysate. Lane 5: Rat colon tissue lysate. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TRAF3 / CRAF1 Control Peptide

TRAF3 / CRAF1
50 µg / €160.00
  Acris Antibodies GmbH

TRAF3 / CRAF1 Control Peptide

TRAF3 / CRAF1
50 µg / €160.00
  Acris Antibodies GmbH

TRAF3 / CRAF1 antibody

TRAF3 / CRAF1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

TRAF3 / CRAF1 antibody

Western Blot analysis of TRAF3 expression in transfected 293T cell line (H00007187-T01) by TRAF3 MaxPab polyclonal antibody.

Lane 1: TRAF3 transfected lysate(62.48 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

TRAF3 / CRAF1 antibody

Western Blot analysis of TRAF3 expression in transfected 293T cell line (H00007187-T02) by TRAF3 MaxPab polyclonal antibody.

Lane 1: TRAF3 transfected lysate(62.48 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

TRAF3 / CRAF1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between TRAF5 and TRAF3 HeLa cells were stained with anti-TRAF5 rabbit purified polyclonal 1:1200 and anti-TRAF3 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 1C5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TRAF3 / CRAF1 antibody

Western blot analysis of TRAF3 in various tumor cell lines. Using TRAF3 polyclonal antibody ( Cat # PAB0276 ) at 1 : 2000. TRAF3 is observed at ~67 kDa. Rabbit Hu, Ms, Rt C, IP, P, WB
50 µl / €390.00
  Abnova Taiwan Corp.

TRAF3 / CRAF1 antibody

Western blot analysis of TRAF3 in NIH/3T3 cell lysate with TRAF3 polyclonal antibody ( Cat # PAB12954 ) at ( A ) 1, and ( B ) 2 µg/ml. Rabbit Hu, Ms WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

TRAF3 / CRAF1 antibody

Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 polyclonal antibody ( Cat # PAB12955 ) at 10 µg/ml. Rabbit Hu, Ms, Rt ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

TRAF3 / CRAF1 antibody

TRAF3 / CRAF1 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

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