Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Dros (Drosophila), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)
Background of TRAF3IP3 / T3JAM antibody
TRAF3-interacting JNK-activating modulator is a protein that in humans is encoded by the TRAF3IP3 gene. The gene encodes a protein that mediates cell growth by modulating the c-Jun N-termil kise sigl transduction pathway. The encoded protein may also interact with a large multiprotein assembly containing the phosphatase 2A catalytic subunit. RAF3IP3 has been shown to interact with STRN; MOBKL3; STK24 and FAM40A.
Ma,X., (2007) Life Sci. 81 (14), 1141-1151
Human Jurkat; WB Suggested Anti-TRAF3IP3 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate. TRAF3IP3 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; TRAF3IP3 antibody - middle region (ARP49942_P050) in Human Jurkat cells using Western Blot
Human 721_B; WB Suggested Anti-TRAF3IP3 Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysate. There is BioGPS gene expression data showing that TRAF3IP3 is expressed in 721_B.; TRAF3IP3 antibody - C-terminal region (ARP49943_P050) in Human 721_B cells using Western Blot
Immunohistochemical analysis of TRAF3IP3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunoprecipitation of TRAF3IP3 from 0.5mg Mouse cortex tissue lysate using 5ug of Anti-TRAF3IP3 Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min Mouse cortex extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70 C; 10ul of each sample was separated on a SDS PAGE gel transferred to a nitrocellulose membrane blocked with 5% BSA and probed with Anti-TRAF3IP3 Antibody.