TRAF6 / RNF85 antibody

Principal name

TRAF6 / RNF85 antibody

Alternative names for TRAF6 / RNF85 antibody

TNF receptor-associated factor 6, Interleukin-1 signal transducer, RING finger protein 85

SwissProt ID

P70196 (Mouse), Q9Y4K3 (Human)

Gene ID

7189 (TRAF6), 22034 (Traf6)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine)

Available hosts

Mouse, Rabbit, Hamster

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of TRAF6 / RNF85 antibody

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein mediates the signaling not only from the members of the TNF receptor superfamily, but also from the members of the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein.The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein mediates the signaling not only from the members of the TNF receptor superfamily, but also from the members of the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. Two alternatively spliced transcript variants encoding identical proteins have been reported.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TRAF6 / RNF85 antibody

  Rabbit anti-TRAF6 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using TRAF6 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRAF6 / RNF85 antibody

  Rabbit Polyclonal anti-TRAF6 antibody  
WB Suggested Anti-TRAF6 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

TRAF6 / RNF85 (C-term) antibody

  Rabbit Polyclonal TRAF6 Antibody  
Western blot analysis of TRAF6 in PC-3 cell lysates with TRAF6 antibody at 1 ug/mL in the presence (A) or absence (B) of 1 ug blocking peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRAF6 / RNF85 antibody

  Anti-TRAF6 Rabbit Polyclonal Antibody  
Predicted band size: 60 kDa . Positive control: A431 cell, human liver cancer tissue and hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: A431 cell lysate Lane2: Human liver cancer tissue lysate Lane3: Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TRAF6 / RNF85 antibody

  Anti-TRAF6 Rabbit Polyclonal Antibody  
Predicted band size: 60 kDa . Positive control: A549 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TRAF6 / RNF85 antibody

  Rabbit Polyclonal Anti-TRAF6 Antibody  
Host: Rabbit; Target Name: TRAF6; Sample Tissue: PANC1 Whole Cell lysates; Antibody Dilution: 3.0ug/ml Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRAF6 / RNF85 antibody

  Rabbit Polyclonal TRAF-6 Antibody  
Western Blot: TRAF6 Antibody [TA336330] - Analysis of TRAF6 in normal Human tissues using this antibody. TRAF6 is observed at ~66 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF6 degradation fragments. Rabbit Purified Bov, Hu, Ms, Rt IP, P, WB
50 µl / €325.00
  OriGene Technologies, Inc.

TRAF6 / RNF85 antibody

  Rabbit Polyclonal TRAF-6 Antibody  
Western Blot: TRAF6 Antibody [TA336357] - Western blot analysis of TRAF6 using TA336357 at 4 ug/ml in A) human thymus cell lysate and B) human testis cell lysate. Rabbit Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRAF6 / RNF85 antibody

  Mouse Monoclonal anti-TRAF6 Antibody  
Hela (Lane 1) and 3T3 (Lane 2) cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with a monoclonal anti-TRAF6 antibody. Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse T2-1SC Purified Hu, Ms WB
0.1 mg / €280.00
  OriGene Technologies, Inc.

TRAF6 / RNF85 antibody

Western blot analysis of TRAF6 expression in L5178Y (1), WR19L (2) and NIH/3T3 (3) using AM26628AF-N. Hamster IgG 1F8 Azide Free Ms IP, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

TRAF6 / RNF85 antibody

Spleen: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TRAF6 / RNF85 antibody

Immunohistochemistry (IHC) analyzes of TRAF6 antibody (Cat.-No.: AP20777PU-N) in paraffin-embedded human testis tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TRAF6 / RNF85 antibody

Human Kidney (formalin-fixed, paraffin-embedded) stained with TRAF6 antibody AP22744PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Purified Hu, Ms IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

TRAF6 / RNF85 antibody

AP26352PU-N: Western blot analysis of TRAF6 in 3T3 cell lysate (Lane 1) and in Jurkat cell lysate (Lane 2 & 3). Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €330.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

TRAF6 - human gene knockout kit via CRISPR

TRAF6 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

TRAF6 - human gene knockout kit via CRISPR

TRAF6 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

TRAF6 - human gene knockout kit via CRISPR

TRAF6 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

TRAF6 - human gene knockout kit via CRISPR

TRAF6 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Traf6 - mouse gene knockout kit via CRISPR

Traf6 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Traf6 - mouse gene knockout kit via CRISPR

Traf6 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

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