TRAF6 / RNF85 antibody

Principal name

TRAF6 / RNF85 antibody

Alternative names for TRAF6 / RNF85 antibody

TNF receptor-associated factor 6, Interleukin-1 signal transducer, RING finger protein 85

SwissProt ID

P70196 (Mouse), Q9Y4K3 (Human)

Gene ID

7189 (TRAF6), 22034 (Traf6)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Mky (Monkey), Por (Porcine), Can (Canine)

Available hosts

Rabbit, Mouse, Hamster

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of TRAF6 / RNF85 antibody

TRAFs (Tumor necrosis factor receptor-associated factors) are cytoplasmic adaptor proteins that associate with tumor necrosis factor receptors (TNFR), thus mediating tumor necrosis factor (TNF) induced signaling. The TRAF family consists of six members (TRAF1-6).1-3 TRAF proteins play an important role in regulating cellular death and cellular response to stress by mediating stimulation of AP-1 family through activation of MAP kinases, including JNK/SAPKs, ERKs and p38s.4 They are also involved in the regulation of inflammatory and anti-apoptotic responses through activation of the transcription factors of NF-kB.5 TRAF6 possesses several binding motifs in CD40, TRANCE-R and IRAK suggesting a crucial role in TRANCE-R signaling. It is involved in the activation of the anti-apoptotic serine/threonine kinase Akt/PKB through TRANCE-R.6 Due to its specific receptor-binding TRAF-C domain, TRAF6 is the only member of the TRAF family that also participates in signal transduction of the interlukin-1 receptor (IL-1R)/Toll-like receptor (TLR) superfamily.1,7,8 TRAF6 plays a crucial role in adaptive immunity, innate immunity, bone homeostasis, perinatal and postnatal survival, and cytokine signaling.9 Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Reacts with the human and mouse TRAF6 proteins. On Western blots, it identifies a band at ~55kD and a band at ~60kD. The two bands may represent the two reported forms of this protein (522 and 530 amino acids). Reactivity has been confirmed with human Hela, HEK293, Jurkat, and mouse NIH3T3 cell lysates.
Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-kB. TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families. TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands. It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) or by other TLR adaptor molecules such as TRIF. Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TRAF6 / RNF85 antibody

Western blot analysis of extracts of various cell lines, using TRAF6 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRAF6 / RNF85 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between TRAF4 and TRAF6 HeLa cells were stained with anti-TRAF4 rabbit purified polyclonal 1:1200 and anti-TRAF6 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2b 1B2 Purified Hu E, WB
50 µg / €370.00
  Abnova Taiwan Corp.

TRAF6 / RNF85 antibody

Hum. Adult Placenta; Host: Rabbit . Target Name: TRAF6 . Sample Tissue: Human Adult Placenta . Antibody Dilution: 1.0ug/ml.; TRAF6 antibody - middle region (ARP30226_P050) in Hum. Adult Placenta cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

TRAF6 / RNF85 (C-term) antibody

Western blot analysis of TRAF6 in PC-3 cell lysates with TRAF6 antibody at 1 ug/mL in the presence (A) or absence (B) of 1 ug blocking peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRAF6 / RNF85 antibody

Predicted band size: 60 kDa . Positive control: A431 cell, human liver cancer tissue and hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: A431 cell lysate Lane2: Human liver cancer tissue lysate Lane3: Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TRAF6 / RNF85 antibody

Predicted band size: 60 kDa . Positive control: A549 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TRAF6 / RNF85 antibody

Western Blot: TRAF6 Antibody [TA336330] - Analysis of TRAF6 in normal Human tissues using this antibody. TRAF6 is observed at ~66 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF6 degradation fragments. Rabbit Purified Bov, Hu, Ms, Rt IP, P, WB
50 µl / €325.00
  OriGene Technologies, Inc.

TRAF6 / RNF85 antibody

Western Blot: TRAF6 Antibody [TA336357] - Western blot analysis of TRAF6 using TA336357 at 4 ug/ml in A) human thymus cell lysate and B) human testis cell lysate. Rabbit Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRAF6 / RNF85 antibody

Western blot analysis of TRAF6 expression in L5178Y (1), WR19L (2) and NIH/3T3 (3) using AM26628AF-N. Hamster IgG 1F8 Azide Free Ms IP, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

TRAF6 / RNF85 antibody

Spleen: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Serum Hu IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TRAF6 / RNF85 antibody

Spleen: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TRAF6 / RNF85 Control Peptide

TRAF6 / RNF85 Rabbit
0.1 mg / €250.00
  Acris Antibodies GmbH

TRAF6 / RNF85 antibody

TRAF6 / RNF85 Rabbit Aff - Purified Hu E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

TRAF6 / RNF85 antibody

Immunohistochemistry (IHC) analyzes of TRAF6 antibody (Cat.-No.: AP20777PU-N) in paraffin-embedded human testis tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TRAF6 / RNF85 antibody

Human Kidney (formalin-fixed, paraffin-embedded) stained with TRAF6 antibody AP22744PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Purified Hu, Ms IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

TRAF6 / RNF85 (194-378) antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bov, Hu, Mky, Por ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

TRAF6 / RNF85 antibody

AP26352PU-N: Western blot analysis of TRAF6 in 3T3 cell lysate (Lane 1) and in Jurkat cell lysate (Lane 2 & 3). Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

TRAF6 / RNF85 Control Peptide

TRAF6 / RNF85
50 µg / €160.00
  Acris Antibodies GmbH

TRAF6 / RNF85 antibody

TRAF6 / RNF85 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

TRAF6 / RNF85 antibody

Immunofluorescence of purified MaxPab antibody to TRAF6 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

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