Transducin alpha-1 chain / GNAT1 antibody

Principal name

Transducin alpha-1 chain / GNAT1 antibody

Alternative names for Transducin alpha-1 chain / GNAT1 antibody

GNATR, Guanine nucleotide-binding protein G(t) subunit alpha-1

SwissProt ID

P04695 (Bovin), P11488 (Human), P20612 (Mouse), Q28300 (Canfa), Q90WX6

Gene ID

2779 (GNAT1), 1467750 (Q9Z6M8), 5140428

Available reactivities

Bov (Bovine), Sh (Sheep), Hu (Human), Ms (Mouse), Ze (Zebrafish), Rt (Rat)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F)

Background of Transducin alpha-1 chain / GNAT1 antibody

Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin.
Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells.

General readings

General / background references:Calvert PD et al. Phototransduction in transgenic mice after targeted deletion of the rod transducin alpha -subunit. Proc Natl Acad Sci U S A 97:13913-8 (2000). PubMed PMID: 11095744
Baylor D How photons start vision. Proc Natl Acad Sci U S A 93:560-5 (1996). PubMed PMID: 8570595

6 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Transducin alpha-1 chain / GNAT1 antibody

Western blot analysis of extracts from COLO cells, using GNAT1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Transducin alpha-1 chain / GNAT1 (C-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded moouse heart tissue reacted with GNAT1 Antibody (C-term) Cat.-No AP51875PU-N, which was  peroxidase conjugated to the secondary antibody and followed by AB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Transducin alpha-1 chain / GNAT1 antibody

Western blot analysis of extracts from COLO cells, using GNAT1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

Transducin alpha-1 chain / GNAT1 antibody

Western blot analysis of extracts from COLO cells, using GNAT1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

Transducin alpha-1 chain / GNAT1 antibody

Western Blot: Transducin alpha Antibody [NB120-3504] - Figure 1 shows a Western blot of Tr alpha on sheep retinal extracts using NB120-3504. Rabbit IgG Aff - Purified Bov, Sh WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Transducin alpha-1 chain / GNAT1 (full length, N-term HIS tag)

Transducin alpha-1 chain / GNAT1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

6 Item(s)

per page
  • LinkedIn