Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Frozen Sections (C)
Background of Transgelin-2 (TAGLN2) antibody
TAGLN2, also known transgelin 2, is one of the earliest markers of differentiated smooth muscle. This protein contains a calponin like repeat and a calponin-homology (CH) domain. It is downregulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. It also binds actin, causing actin fibers to gel within minutes of binding. Binding of transgelin to actin occurs at a ratio of 1:6 actin monomers. Recombinant human TAGLN2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Immunohistochemical of paraffin-embedded human colon cancer using 10234-2-AP(TAGLN2 antibody) at dilution of 1:100 (under 10x lens)
Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: A549 cells were fixed in ice-cold MeOH for 5 min.Green: Transgelin 2 protein stained by Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 μm.
Transgelin 2 antibody detects Transgelin 2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin 2 antibody (GTX115082) diluted by 1:1000.
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.