TRAP1 / HSP75 antibody

Principal name

TRAP1 / HSP75 antibody

Alternative names for TRAP1 / HSP75 antibody

TRAP-1, TNFR-associated protein 1, HSP 75, Heat shock protein 75 kDa mitochondrial, Tumor necrosis factor type 1 receptor-associated protein

SwissProt ID

Q12931 (Human), Q2TBI4 (Bovin), Q5XHZ0 (Rat), Q9CQN1 (Mouse)

Gene ID

10131 (TRAP1), 101314 (6720456B07Rik)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Can (Canine), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Flow Cytometry (F), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of TRAP1 / HSP75 antibody

The 90 kDa heat shock protein (hsp90) family of molecular chaperones is a highly conserved family of proteins that play an important physiological role. Hsp90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of hsp90 is TRAP1. Like hsp90, TRAP1 is found to be associated with numerous proteins involved in diverse actions. Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting presequence strongly implicates TRAP1 as a mitochondrial matrix protein. Immunofluorescence staining of TRAP1 in PC-3-M cells with this antibody produces a pattern consistent with mitochondrial staining. Immunoprecipitation of TRAP1 using this antibody fails to co-precipitate p23, Hop, or CyP40 suggesting TRAP1?s inability to associate with these co-chaperones.

General readings

General / background references:Johnson BD et al. Hsp90 chaperone activity requires the full-length protein and interaction among its multiple domains. J Biol Chem 275:32499-507 (2000). PubMed PMID: 10913439
Felts SJ et al. The hsp90-related protein TRAP1 is a mitochondrial protein with distinct functional properties. J Biol Chem 275:3305-12 (2000). PubMed PMID: 10652318

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TRAP1 / HSP75 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504202) Mouse IgG2b OTI1H8 Purified Can, Hu, Mky, Rt F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRAP1 / HSP75 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TRAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504224) Mouse IgG1 OTI1A1 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRAP1 / HSP75 antibody

Predicted band size: 80 kDa . Positive control: Hela and 293T cell, mouse kidney tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: 293T cell lysate Lane3: Mouse Kidney lysate Lysates: 30 ug per lane Primary antibody: 1/1500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TRAP1 / HSP75 antibody

Predicted band size: 80 kDa . Positive control: K562, Jurkat and 293T cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: K562 cell lysate Lane2: Jurkat cell lysate Lane3: 293T cell lysate Lysates: 30 ug per lane Primary antibody: 1/1050 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TRAP1 / HSP75 antibody

Western blot analysis of TRAP1 in heat shocked HeLa cells, using a 1:1000 dilution of TA326389. Mouse IgG2a TRAP1-6 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRAP1 / HSP75 antibody

WB Suggested Anti-TRAP1 Antibody; Titration: 0.5 ug/ml; Positive Control: HepG2 Whole CellTRAP1 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

TRAP1 / HSP75 antibody

WB Suggested Anti-TRAP1 Antibody Titration: 0.5 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysateTRAP1 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRAP1 / HSP75 antibody

TRAP1 / HSP75 Rabbit Purified Hu, Ms, Rt WB
0.1 ml / €360.00
  Acris Antibodies GmbH

TRAP1 / HSP75 antibody

Immunofluorescence of purified MaxPab antibody to TRAP1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

TRAP1 / HSP75 antibody

TRAP1 / HSP75 Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

TRAP1 / HSP75 antibody

TRAP1 / HSP75 Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

TRAP1 / HSP75 antibody

TRAP1 / HSP75 Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

TRAP1 / HSP75 antibody

Human HepG2; WB Suggested Anti-TRAP1 Antibody. . Titration: 0.5 ug/ml. . Positive Control: HepG2 Whole Cell. TRAP1 is supported by BioGPS gene expression data to be expressed in HepG2.; TRAP1 antibody - N-terminal region (AVARP00012_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Chk, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

TRAP1 / HSP75 antibody

Human kidney; TRAP1 antibody - N-terminal region (AVARP00012_T100) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified Hu P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

TRAP1 / HSP75 antibody

Figure 1 shows immunolocalization of TRAP1 in PC-3-M cells using NB 300-555. Mouse IgG1 TRAP1-6 Ig Fraction Hu ICC/IF, IP, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

TRAP1 / HSP75 antibody

Western Blot: TRAP1 Antibody [NBP1-32097]- (30 µg of whole cell lysate) A:Hep G2 B:MOLT4 7.5% SDS PAGE diluted at 1:1500 Rabbit IgG Hu ICC/IF, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TRAP1 / HSP75

TRAP1 / HSP75 Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

TRAP1 / HSP75

TRAP1 / HSP75 Human
10 µg / €380.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

TRAP1 (Human) IP-WB Antibody Pair

TRAP1 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

TRAP1 293T Cell Transient Overexpression Lysate(Denatured)

TRAP1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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