Trefoil factor 1 / pS2 antibody

Principal name

Trefoil factor 1 / pS2 antibody

Alternative names for Trefoil factor 1 / pS2 antibody

TFF1, BCEI, Breast cancer estrogen-inducible protein, PNR-2, Polypeptide P1.A

SwissProt ID

P04155 (Human), Q08423 (Mouse), Q63467 (Rat), Q863T4 (Canfa)

Gene ID

7031 (TFF1), 70316 (Ndufab1), 100041004 (Gm3090)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)

Background of Trefoil factor 1 / pS2 antibody

TFF1(Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to that of nephrocalcin, and inhibition was dose dependent and inhibited by TFF1 antisera, particularly by antisera directed to the TFF1 C terminus. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls.This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.

General readings

General / background references:Mori K et al. Complete primary structure of the human estrogen-responsive gene (pS2) product. J Biochem (Tokyo) 107:73-6 (1990). PubMed PMID: 2185238
Rio MC et al. Breast cancer-associated pS2 protein: synthesis and secretion by normal stomach mucosa. Science 241:705-8 (1988). PubMed PMID: 3041593
Jeltsch JM et al. Structure of the human oestrogen-responsive gene pS2. Nucleic Acids Res 15:1401-14 (1987). PubMed PMID: 3822834
Masiakowski P et al. Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line. Nucleic Acids Res 10:7895-903 (1982). PubMed PMID: 6897676

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Trefoil factor 1 / pS2 (57-84) antibody

Formalin-Fixed, Paraffin-Embedded human breast Ca stained with Trefoil factor 1 / pS2 Antibody Clone GE2. Mouse IgG1 GE2 (same as R47/94) Purified Hu, Mky C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Trefoil factor 1 / pS2 (57-84) antibody

Formalin-Fixed, Paraffin-Embedded human breast Ca stained with Trefoil factor 1 / pS2 Antibody Clone GE2. Mouse IgG1 GE2 (same as R47/94) Purified Hu, Mky C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Trefoil factor 1 / pS2 (57-84) antibody

Formalin-Fixed, Paraffin-Embedded human breast Ca stained with Trefoil factor 1 / pS2 Antibody Clone GE2. Mouse IgG1 GE2 (same as R47/94) Purified Hu, Mky C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Trefoil factor 1 / pS2 antibody

Formalin-paraffin human breast Ca Stained with pS2 Monoclonal Antibody (Clone SPM573). Mouse IgG1 SPM573 Purified Hu, Mky F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Trefoil factor 1 / pS2 antibody

Formalin-paraffin human breast Ca Stained with pS2 Monoclonal Antibody (Clone SPM573). Mouse IgG1 SPM573 Purified Hu, Mky F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Trefoil factor 1 / pS2 antibody

Formalin-paraffin human breast Ca Stained with pS2 Monoclonal Antibody (Clone SPM573). Mouse IgG1 SPM573 Purified Hu, Mky F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Trefoil factor 1 / pS2 antibody

Western blot analysis using 50 ng recombinant human TFF-1. Rabbit Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Trefoil factor 1 / pS2 antibody

Predicted band size: 9 kDa . Positive control: Human fetal kidney tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%+12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Trefoil factor 1 / pS2 antibody

Predicted band size: 9 kDa . Positive control: Mouse intestinum tenue tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%+12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Trefoil factor 1 / pS2 antibody

WB Suggested Anti-TFF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Stomach Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Trefoil factor 1 / pS2 antibody

Figure 1. Immunohistochemical staining using PS2 Estrogen-Regulated Proteini antibody (AM05313PU-N) on formalin fixed, paraffin embedded human breast carcinoma. Mouse IgG1 pS2.1 Purified Hu P
0.1 mg / €570.00
  Acris Antibodies GmbH

Trefoil factor 1 / pS2 antibody

Human Trachea Frozen Section stained with TFF1 Antibody Cat.-No  AM21051PU-N (Clone C-3D5) Mouse IgG1 C-3D5 Aff - Purified Hu C, P
0.1 mg / €420.00
  Acris Antibodies GmbH

Trefoil factor 1 / pS2 antibody

Trefoil factor 1 / pS2 Mouse IgG1 4G2-2G4 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

Trefoil factor 1 / pS2 antibody

Detection limit for recombinant GST tagged TFF1 is approximately 0.3ng/ml as a capture antibody. Mouse IgG1 3H5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Trefoil factor 1 / pS2 antibody

Detection limit for recombinant GST tagged TFF1 is approximately 0.3ng/ml as a capture antibody. Mouse IgG1 M1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Trefoil factor 1 / pS2 antibody

Western Blot analysis of TFF1 expression in transfected 293T cell line by TFF1 monoclonal antibody (M04), clone M2.

Lane 1: TFF1 transfected lysate(9.1 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 M2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Trefoil factor 1 / pS2 antibody

Trefoil factor 1 / pS2 Mouse IgG1 M2 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

Trefoil factor 1 / pS2 antibody

Immunohistochemica lstaining using TFF1 monoclonal antibody, clone pS2.1 ( Cat # MAB2015 ) on formalin fixed, paraffin embedded human breast carcinoma. Hu
0.1 mg / €640.00
  Abnova Taiwan Corp.

Trefoil factor 1 / pS2 antibody

Trefoil factor 1 / pS2 Rabbit Hu, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Trefoil factor 1 / pS2 antibody

Human Stomach; WB Suggested Anti-TFF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Stomach; TFF1 antibody - middle region (ARP45808_P050) in Human Stomach cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

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