Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Trefoil factor 3 / TFF3 antibody
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. TFF3 is expressed in goblet cells of the intestines and colon. The TFF3 gene consists of 3 exons, with exon 2 encoding the trefoil domain. The TFF1, TFF2, and TFF3 genes are located within a 55 kb region, suggesting they evolved from a common ancestor by duplication.
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Formalin-fixed and paraffin embedded: mouse small intestine tissue labeled with Anti-TFF3 Polyclonal Antibody (bs-0535R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
HT-29 cells probed with Rabbit Anti-TFF3 Polyclonal Antibody (bs-0535R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H L) Alexa Fluor 488 Conjugated Secondary Antibody.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in goblet cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFF3 (RC223558, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFF3.