TRERF1 antibody
Principal name
TRERF1 antibody
Alternative names for TRERF1 antibody
Transcriptional-regulating factor 1, BCAR2, RAPA, TREP132, Transcriptional-regulating protein 132, Zinc finger transcription factor TReP-132, Breast cancer anti-estrogen resistance 2
Ncbi ID
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F), Frozen Sections (C)
Background of TRERF1 antibody
The transcriptional regulating protein of 132 kDa (TReP-132/TRERF1) was identified as a transcription factor that interacts with and activates the promoter of the CYP11A1 gene, a gene that encodes cytochrome P450 side chain cleavage (P450scc) and catalyzes the first step in steroidgenesis. TRep-132 can function as a nuclear receptor co-activator and has been shown to interact with Sp1 and the progesterone receptor (PR) to suppress growth via activation of the gene promoters of the cyclin-dependent kinase inhibitors, p21 and p27.
Immunohistochemistry-Paraffin: TRERF1 Antibody [NB100-81654] - See Notes, IHC Caption
Western Blot: TReP-132 Antibody [NB100-81654] - Detection of Human TReP-132/TRERF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TReP-132/TRERF1 antibody NB100-81654 used for IP at 3 mcg/mg lysate. TReP-132/TRERF1 was also immunoprecipitated by rabbit anti-TReP-132/TRERF1 antibody NB100-81655, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.
WB and IP: TReP-132 Antibody [NB100-81655] -Detection of Human TReP-132/TRERF1 on HeLa whole cell lysate using NB100-81655. TReP-132/TRERF1 was also immunoprecipitated by rabbit anti-TReP-132/TRERF1 antibody NB100-81654.
MCF7 cells were subjected to SDS PAGE followed by western blot with 20062-1-AP(TRERF1 antibody) at dilution of 1:300
MCF7 cells were subjected to SDS PAGE followed by western blot with 20062-1-AP(TRERF1 antibody) at dilution of 1:300
Mouse Intestine; WB Suggested Anti-Trerf1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Intestine; Trerf1 antibody - N-terminal region (ARP39765_P050) in Mouse Intestine cells using Western Blot
Western blot analysis of TREF1 Antibody (C-term) (AP54355PU-N) in K562 cell line lysates (35 µg/lane). TREF1 (arrow) was detected using the purified Pab.
TREF1 Antibody (C-term) (AP54355PU-N) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TREF1 antibody. The picture on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315608 | TRERF1 antibody |
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Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€325.00
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| OriGene Technologies, Inc. | |||||||
| TA315609 | TRERF1 antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| TA325102 | TRERF1 (C-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | F, WB |
0.4 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| AP32250PU-N | TRERF1 (C-term) antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu, Ms | E, P |
50 µg /
€470.00
|
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| Acris Antibodies GmbH | |||||||
| H00055809-B01P | TRERF1 antibody |
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 |
Mouse | Hu | WB |
0.5 mg /
€1,400.00
|
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| Abnova Taiwan Corp. | |||||||
| C11526-1 | TRERF1 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms | E, P |
50 µg /
€205.00
|
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| Assay Biotech | |||||||
| C11526-2 | TRERF1 antibody |
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|
Rabbit | Aff - Purified | Hu, Ms | E, P |
0.1 mg /
€310.00
|
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| Assay Biotech | |||||||
| ARP39765_P050 | TRERF1 (N-term) antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms | WB |
50 µg /
€350.00
|
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| AVIVA Systems Biology | |||||||
| NB100-81654 | TRERF1 antibody |
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![Immunohistochemistry-Paraffin: TRERF1 Antibody [NB100-81654] - See Notes, IHC Caption](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/TRERF1 Antibody-Immunohistochemistry-Paraffin-NB100-81654-img0001.jpg) |
Rabbit | IgG | Aff - Purified | Hu | IP, P |
0.1 mg /
€440.00
|
|
| Novus Biologicals Inc. | |||||||
| +1 additional image |
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| NB100-81655 | TRERF1 antibody |
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![WB and IP: TReP-132 Antibody [NB100-81655] -Detection of Human TReP-132/TRERF1 on HeLa whole cell lysate using NB100-81655. TReP-132/TRERF1 was also immunoprecipitated by rabbit anti-TReP-132/TRERF1 antibody NB100-81654.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/p_81655_1.jpg) |
Rabbit | IgG | Aff - Purified | Hu | IP, WB |
0.1 mg /
€440.00
|
|
| Novus Biologicals Inc. | |||||||
| NBP1-66321-0.1 | TRERF1 antibody |
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Rabbit | IgG | Aff - Purified | Hu, Ms | E, P |
0.1 mg /
€360.00
|
|
| Novus Biologicals Inc. | |||||||
| NBP1-66321-50 | TRERF1 antibody |
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Rabbit | IgG | Aff - Purified | Hu, Ms | E, P |
50 µg /
€290.00
|
|
| Novus Biologicals Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| H00055809-Q01-10 | TRERF1 |
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Human | 12.5% SDS-PAGE Stained with Coomassie Blue |
10 µg /
€380.00
|
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| Abnova Taiwan Corp. | |||||
| H00055809-Q01-25 | TRERF1 |
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Human | 12.5% SDS-PAGE Stained with Coomassie Blue |
25 µg /
€510.00
|
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| Abnova Taiwan Corp. | |||||
