TRIB3 antibody
Principal name
TRIB3 antibody
Alternative names for TRIB3 antibody
Tribbles homolog 3, TRB3, TRB-3, NIPK, SKIP3
Gene ID
57761 (TRIB3)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Frozen Sections (C)
Background of TRIB3 antibody
A novel inhibitor of cell division, Tribbles, blocks mitosis at a critical point in Drosophila morphogenesis. Recently, mammalian homologuesof Tribbles (Trbs) have been identified that control mitogen activated protein kinase (MAPK) activity. TRIB3 is a putative protein kinase that is induced by the transcription factor NFkappaB. TRIB3 is a negative regulator of NFkappaB and can also sensitize cells to TNF and TRAIL induced apoptosis. In addition, this protein can negatively regulate the cell survival serine threonine kinase AKT1.TRIB 3 disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the Thr-308 phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non neuronal cells. Does not display kinase activity.TRIB3 interacts with AKT1, AKT2, ATF4, MAP2K1 and MAP2K7.Its highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. TRIB3 is induced by hypoxia. It contains 1 protein kinase domain.
General readings
Du K. et al. (2003) Science. 300:1574-1577.Hegedus Z. et al. (2006) Cell Mol Life Sci. 63:1632-1641.
Immunofluorescence of monoclonal antibody to TRIB3 on A-431 cell. [antibody concentration 10 ug/ml]
Western blot analysis of TRIB3 over-expressed 293 cell line, cotransfected with TRIB3 Validated Chimera RNAi ( Cat # H00057761-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIB3 monoclonal antibody (M03) clone 1H2 (Cat # H00057761-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of TRIB3 expression in transfected 293T cell line by TRIB3 monoclonal antibody (M03), clone 1H2.Lane 1: TRIB3 transfected lysate(39.6 KDa).Lane 2: Non-transfected lysate.
TRIB3 monoclonal antibody (M05), clone 2F7 Western Blot analysis of TRIB3 expression in HepG2 ( Cat # L019V1 ).
Detection limit for recombinant GST tagged TRIB3 is approximately 3ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to TRIB3 on HeLa cell. [antibody concentration 10 ug/ml]
TRIB3 monoclonal antibody (M06), clone 2G5 Western Blot analysis of TRIB3 expression in HepG2 ( Cat # L019V1 ).
Detection limit for recombinant GST tagged TRIB3 is approximately 3ng/ml as a capture antibody.
Western blot analysis of TRIB3 in human liver lysate.
Western Blot: TRIB3/SKIP3/SINK Antibody [NB100-56398] - Western blot analysis of TRIB3 in human liver lysate.
Western blot analysis of TRIB3 in human liver lysate ( TRIB3 polyclonal antibody, Cat # PAB0336 ) .
Western Blot analysis of TRIB3 expression in transfected 293T cell line by TRIB3 polyclonal antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with TRIB3 polyclonal antibody ( Cat # PAB4034 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
The TRIB3 polyclonal antibody ( Cat # PAB4034 ) is used in Western blot to detect TRIB3 in Jurkat cell lysate.
The TRIB3 polyclonal antibody ( Cat # PAB4035 ) is used in Western blot to detect TRIB3 in mouse skeletal muscle tissue lysate.
Western Blot analysis of TRIB3 expression in transfected 293T cell line (H00057761-T01) by TRIB3 MaxPab polyclonal antibody.Lane 1: TRIB3 transfected lysate(39.38 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
(Left)The anti-NPK Pab is used in Western blot to detect NPK in Jurkat cell lysate. (Right)Western blot analysis of NPK(arrow) using rabbit polyclonal NPK Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRIB3 gene (Lane 2) (Origene Technologies).
The anti-NPK Pab is used in Western blot to detect NPK in mouse skeletal muscle tissue lysate.
Western Blot analysis of TRIB3 expression in transfected 293T cell line (H00057761-T02) by TRIB3 MaxPab polyclonal antibody.Lane 1: TRIB3 transfected lysate(39.60 KDa).Lane 2: Non-transfected lysate.
Western Blot: TRIB3 Lysate [NBL1-17273] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for TRIB3
Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using TRB-3 (GTX113991) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TRB-3 (GTX113991) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 10% SDS PAGE GTX113991 diluted at 1:1000
Sample (15 ug of whole cell lysate) A: HUVEC GTX113991 diluted at 1:5000
Western Blot: TRIB3 Antibody (EPR3151Y) [NBP1-40561] - Western blot analysis on (A) 293T treated with Ionomycin and TPA and (B) 293T cell lysates using anti-TRIB3, dilution 1:10,000.
Mouse liver tissue were subjected to SDS PAGE followed by western blot with 13300-1-AP(TRIB3 antibody) at dilution of 1:600
IP Result of anti-TRIB3 (IP:13300-1-AP, 4ug; Detection:13300-1-AP 1:500) with mouse liver tissue lysate 4000ug.
Immunohistochemistry-Paraffin: TRIB3 Antibody [NBP1-88291] - staining of human pancreas shows strong cytoplasmic positivity in subset of exocrine glandular cells.
Immunofluorescence: TRIB3 Antibody [NBP1-88291] - 431 shows positivity in nuclei but not nucleoli.
Western Blot: TRIB3 Antibody [NBP1-88291] Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
Western blot analysis of TRIB3 expression in HuvEc (A); HepG2 (B); SP2/0 (C); PC12 (D) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807482)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB3 (RC206687, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB3.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807483)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807483)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807483)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807483)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB3 (RC206687, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB3.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807484)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807484)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB3 (RC206687, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB3.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807540)(1:150)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807611)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB3 (RC206687, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB3.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807612)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807612)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB3 (RC206687, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB3.
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-TRIB3 monoclonal antibody at 1:200 dilution.
- Quick-Filters:
- Highly Validated (2)
- Overnight Delivery (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA807483 | TRIB3 antibody |
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Mouse | IgG1 | OTI3C6 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody!
|
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| TA807612 | TRIB3 antibody |
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 |
Mouse | IgG1 | OTI3F3 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA317675 | TRIB3 antibody |
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 |
Rabbit | IgG | Purified | Bov, Can, Hu, Mky, Por, Rb |
50 µg /
€345.00
|
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| OriGene Technologies, Inc. | |||||||
| TA317676 | TRIB3 antibody |
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 |
Rabbit | IgG | Purified | Hu, Mky | P |
50 µg /
€345.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA344735 | TRIB3 antibody |
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 |
Rabbit | IgG | Purified | Bov, Can, GP, Hu, Por, Rb | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA344736 | TRIB3 antibody |
||||||
 |
Rabbit | IgG | Purified | Hu | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA349143 | TRIB3 antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA807482 | TRIB3 antibody |
||||||
 |
Mouse | IgG1 | OTI3F6 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA807484 | TRIB3 antibody |
||||||
 |
Mouse | IgG1 | OTI1F6 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA807540 | TRIB3 antibody |
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 |
Mouse | IgG1 | OTI2D11 | Purified | Hu | P |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA807611 | TRIB3 antibody |
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 |
Mouse | IgG1 | OTI3G4 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| AP15157PU-N | TRIB3 (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
Regular Price: 0.4 ml / €370.00 Special Price: 0.4 ml / €231.00 |
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| AP15158PU-N | TRIB3 (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€370.00
|
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| Acris Antibodies GmbH | |||||||
| AP55357PU-N | TRIB3 antibody |
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 |
Rabbit | Purified | Hu, Ms, Rt | C, E, WB |
0.1 mg /
€420.00
|
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| Acris Antibodies GmbH | |||||||
| H00057761-B01P | TRIB3 antibody |
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 |
Mouse | Purified | Hu | E(detection), WB |
50 µg /
€370.00
|
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| Abnova Taiwan Corp. | |||||||
| H00057761-D01P | TRIB3 antibody |
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 |
Rabbit | Purified | Hu | E(detection), WB |
0.1 mg /
€380.00
|
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| Abnova Taiwan Corp. | |||||||
| H00057761-M03 | TRIB3 antibody |
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![Immunofluorescence of monoclonal antibody to TRIB3 on A-431 cell. [antibody concentration 10 ug/ml]](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/H00057761-M03-4-4-1-L.jpg) |
Mouse | IgG1 | 1H2 | Purified | Hu | E, ICC/IF, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| +2 additional images |
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| H00057761-M05 | TRIB3 antibody |
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 |
Mouse | IgG1 | 2F7 | Purified | Hu | E, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| H00057761-M06 | TRIB3 antibody |
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![Immunofluorescence of monoclonal antibody to TRIB3 on HeLa cell. [antibody concentration 10 ug/ml]](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/H00057761-M06-4-1-1-L.jpg) |
Mouse | IgG1 | 2G5 | Purified | Hu | E, ICC/IF, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| +2 additional images |
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| H00057761-M07 | TRIB3 antibody |
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Mouse | IgG1 | 3D7 | Purified | Hu | E, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
