TRIB3 antibody
Principal name
TRIB3 antibody
Alternative names for TRIB3 antibody
Tribbles homolog 3, TRB3, TRB-3, NIPK, SKIP3
Ncbi ID
9606, NP_066981, NP_066981.2, NM_021158, NP_780302.2, BC012955, NM_144755, NM_175093
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Frozen Sections (C)
Background of TRIB3 antibody
- Kit Component:
- - KN318178G1, Trib3 gRNA vector 1 in pCas-Guide vector
- KN318178G2, Trib3 gRNA vector 2 in pCas-Guide vector
- KN318178D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Du K. et al. (2003) Science. 300:1574-1577.Hegedus Z. et al. (2006) Cell Mol Life Sci. 63:1632-1641.
Immunofluorescence of monoclonal antibody to TRIB3 on A-431 cell. [antibody concentration 10 ug/ml]
Western blot analysis of TRIB3 over-expressed 293 cell line, cotransfected with TRIB3 Validated Chimera RNAi ( Cat # H00057761-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIB3 monoclonal antibody (M03) clone 1H2 (Cat # H00057761-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of TRIB3 expression in transfected 293T cell line by TRIB3 monoclonal antibody (M03), clone 1H2.Lane 1: TRIB3 transfected lysate(39.6 KDa).Lane 2: Non-transfected lysate.
TRIB3 monoclonal antibody (M05), clone 2F7 Western Blot analysis of TRIB3 expression in HepG2 ( Cat # L019V1 ).
Detection limit for recombinant GST tagged TRIB3 is approximately 3ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to TRIB3 on HeLa cell. [antibody concentration 10 ug/ml]
TRIB3 monoclonal antibody (M06), clone 2G5 Western Blot analysis of TRIB3 expression in HepG2 ( Cat # L019V1 ).
Detection limit for recombinant GST tagged TRIB3 is approximately 3ng/ml as a capture antibody.
Western blot analysis of TRIB3 in human liver lysate ( TRIB3 polyclonal antibody, Cat # PAB0336 ) .
Formalin-fixed and paraffin-embedded human cancer tissue reacted with TRIB3 polyclonal antibody ( Cat # PAB4034 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
The TRIB3 polyclonal antibody ( Cat # PAB4034 ) is used in Western blot to detect TRIB3 in Jurkat cell lysate.
The TRIB3 polyclonal antibody ( Cat # PAB4035 ) is used in Western blot to detect TRIB3 in mouse skeletal muscle tissue lysate.
Western Blot analysis of TRIB3 expression in transfected 293T cell line (H00057761-T01) by TRIB3 MaxPab polyclonal antibody.Lane 1: TRIB3 transfected lysate(39.38 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
(Left)The anti-NPK Pab is used in Western blot to detect NPK in Jurkat cell lysate. (Right)Western blot analysis of NPK(arrow) using rabbit polyclonal NPK Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRIB3 gene (Lane 2) (Origene Technologies).
The anti-NPK Pab is used in Western blot to detect NPK in mouse skeletal muscle tissue lysate.
Western Blot analysis of TRIB3 expression in transfected 293T cell line (H00057761-T02) by TRIB3 MaxPab polyclonal antibody.Lane 1: TRIB3 transfected lysate(39.60 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of TRIB3 expression in HuvEc (A); HepG2 (B); SP2/0 (C); PC12 (D) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807482)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB3 (RC206687, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB3.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807483)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807483)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807483)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807483)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB3 (RC206687, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB3.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807484)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807484)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB3 (RC206687, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB3.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807540)(1:150)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807611)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB3 (RC206687, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB3.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807612)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807612)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB3 (RC206687, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB3.
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-TRIB3 monoclonal antibody at 1:200 dilution.
Anti-TRIB3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-TRIB3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
WB Suggested Anti-TRIB3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: ACHN cell lysate
Host: Rabbit; Target Name: TRIB3; Sample Tissue: Fetal Kidney lysates; Antibody Dilution: 1.0 ug/ml
Western blot analysis of TRB3 in mouse kidney tissue lysate with TRB3 antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry of TRB3 in mouse liver tissue with TRB3 antibody at 5 ug/mL.
Immunofluorescence of TRB3 in mouse liver tissue with TRB3 antibody at 20 ug/mL.
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA807483 | TRIB3 antibody |
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| TRIB3 mouse monoclonal antibody, clone OTI3C6 (formerly 3C6) | |||||||
 |
Mouse | IgG1 | OTI3C6 | Purified | Hu | P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody!
|
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| TA807612 | TRIB3 antibody |
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| TRIB3 mouse monoclonal antibody, clone OTI3F3 (formerly 3F3) | |||||||
 |
Mouse | IgG1 | OTI3F3 | Purified | Hu | P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA317675 | TRIB3 antibody |
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| TRB3 / TRIB3 Rabbit Polyclonal (C-Terminus) Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Hu, Mky, Por, Rb |
50 µg /
€400.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA317676 | TRIB3 antibody |
||||||
| TRB3 / TRIB3 Rabbit Polyclonal (N-Terminus) Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Mky | P |
50 µg /
€400.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA344735 | TRIB3 antibody |
||||||
| Rabbit Polyclonal Anti-TRIB3 Antibody - N-terminal region | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, GP, Hu, Por, Rb | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA344736 | TRIB3 antibody |
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| Rabbit Polyclonal Anti-TRIB3 Antibody - N-terminal region | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA349143 | TRIB3 antibody |
||||||
| Rabbit Polyclonal Anti-TRB3 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€382.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA807482 | TRIB3 antibody |
||||||
| TRIB3 mouse monoclonal antibody, clone OTI3F6 (formerly 3F6) | |||||||
 |
Mouse | IgG1 | OTI3F6 | Purified | Hu | P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA807484 | TRIB3 antibody |
||||||
| TRIB3 mouse monoclonal antibody, clone OTI1F6 (formerly 1F6) | |||||||
 |
Mouse | IgG1 | OTI1F6 | Purified | Hu | P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA807540 | TRIB3 antibody |
||||||
| TRIB3 mouse monoclonal antibody, clone OTI2D11 (formerly 2D11) | |||||||
 |
Mouse | IgG1 | OTI2D11 | Purified | Hu | P |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA807611 | TRIB3 antibody |
||||||
| TRIB3 mouse monoclonal antibody, clone OTI3G4 (formerly 3G4) | |||||||
 |
Mouse | IgG1 | OTI3G4 | Purified | Hu | P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| AP15157PU-N | TRIB3 (N-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€370.00
|
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| AP15158PU-N | TRIB3 (C-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€370.00
|
|
| Acris Antibodies GmbH | |||||||
| AP55357PU-N | TRIB3 antibody |
||||||
 |
Rabbit | Purified | Hu, Ms, Rt | C, E, WB |
0.1 mg /
€420.00
|
||
| Acris Antibodies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN318178 | Trib3 - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN318178BN | Trib3 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN318178LP | Trib3 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN318178RB | Trib3 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| AR51742PU-N | TRIB3 (1-358, His-tag) |
||||
 |
Human | Purified | > 85 % by SDS - PAGE | E. coli |
0.25 mg /
€730.00
|
| Acris Antibodies GmbH | |||||
| AR51742PU-S | TRIB3 (1-358, His-tag) |
||||
|
Human | Purified | > 85 % by SDS - PAGE | E. coli |
50 µg /
€300.00
|
| Acris Antibodies GmbH | |||||
