TRIM2 / RNF86 antibody
Principal name
TRIM2 / RNF86 antibody
Alternative names for TRIM2 / RNF86 antibody
KIAA0517, RING finger protein 86, Tripartite motif-containing protein 2
Ncbi ID
9606, NP_001123539, ADZ16100.1, NP_001123539.1, NM_001130067, NP_109631.1, NM_030706, NM_015271
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)
Background of TRIM2 / RNF86 antibody
- Kit Component:
- - KN318189G1, Trim2 gRNA vector 1 in pCas-Guide vector
- KN318189G2, Trim2 gRNA vector 2 in pCas-Guide vector
- KN318189D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
TRIM2 polyclonal antibody ( Cat # PAB7498 ) ( 0.5 µg/ml ) staining of rat brain lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP31074PU-N TRIM2 antibody staining of Rat Brain lysate at 0.5 mg/ml (35 µg protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC203947, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRIM2 monoclonal antibody.
Flow cytometric Analysis of Hela cells, using anti-TRIM2 antibody(TA501551),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC229368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.
Flow cytometric Analysis of Hela cells, using anti-TRIM2 antibody(TA501552),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-TRIM2 antibody(TA501552),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-TRIM2 mouse monoclonal antibody (TA501553) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TRIM2(RC203947).
Immunofluorescent staining of HeLa cells using anti-TRIM2 mouse monoclonal antibody (TA501553).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC203947, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC229368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC203947, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.
HEK293T cells transfected with either RC203947 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TRIM2 antibody(TA501559), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC229368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.
HEK293T cells transfected with either RC203947 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TRIM2 antibody(TA501560), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRIM2 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC229368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.
HEK293T cells transfected with either RC203947 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TRIM2 antibody(TA501606), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-TRIM2 antibody(TA501606),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Hela cells, using anti-TRIM2 antibody(TA501606),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC203947, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.
HEK293T cells transfected with either RC203947 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TRIM2 antibody(TA502192), and then analyzed by flow cytometry.
TA305745 (0.5ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP31074PU-N | TRIM2 / RNF86 (758-771) antibody |
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Goat | Aff - Purified | Bov, Bt, Can, Chk, Elephant, Eq, Hst, Hu, Mky, Ms, Platypus, Rb, Rt, Xen, Ze | E, P, WB |
50 µg /
€380.00
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
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