TRIM25 / RNF147 antibody

Principal name

TRIM25 / RNF147 antibody

Alternative names for TRIM25 / RNF147 antibody

ZNF147, EFP, RING finger protein 147, Tripartite motif-containing protein 25, Zinc finger protein 147, Estrogen-responsive finger protein

SwissProt ID

Q14258 (Human), Q61510 (Mouse)

Gene ID

7706 (TRIM25)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rb (Rabbit), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP)

Background of TRIM25 / RNF147 antibody

EFP (estrogen-responsive finger protein) is a transcription factor, the content of which is regulated by estrogen. It has been identified as a member of the RING finger family, a family of proteins containing a Zn2+ binding domain designated the C3HC4 or RING finger. EFP also contains two B box domains and a coiled-coil region (a transactivation domain), which are characteristic of a subgroup of the RING finger family. Estrogen regulates the growth, differentiation and function of target cells in a variety of tissues; however, few genes have been shown to be directly regulated by estrogen. It has been speculated that EFP may mediate estrogen activity in a signaling cascade in which estrogen-ER binding to the estrogen responsive element (ERE) downstream of the EFP gene upregulates EFP gene expression. The EFP gene product may then activate transcription of secondary estrogen responsive genes. Additional studies indicate that the EFP promoter may be regulated by multiple elements and their interacting factors.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TRIM25 / RNF147 (C-term) antibody

  Rabbit Polyclonal TRIM25 Antibody  
Western blot analysis of TRIM25 in HeLa cell lysate in (A) the absence and (B) presence of blocking peptide with TRIM25 antibody at 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRIM25 / RNF147 (N-term) antibody

  Rabbit Polyclonal TRIM25 Antibody  
Western blot analysis of TRIM25 in human thymus tissue lysate in (A) the absence and (B) presence of blocking peptide with TRIM25 antibody at 1 ug/mL. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRIM25 / RNF147 antibody

  Rabbit polyclonal anti-ZNF147 antibody  
Western blot analysis of extracts from K562 cells, using ZNF147 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TRIM25 / RNF147 antibody

  Rabbit polyclonal TRIM25 Antibody (Center)  
TRIM25  Antibody (Center) (Cat. #TA324607) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TRIM25  antibody detected the TRIM25  protein (arrow). Rabbit IgG Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

TRIM25 / RNF147 antibody

  Rabbit Polyclonal anti-TRIM25 Antibody  
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with polyclonal anti-TRIM25 antibody (Clone Poly6476). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system. Rabbit IgG Poly6476 Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TRIM25 / RNF147 antibody

  Rabbit Polyclonal Anti-TRIM25 Antibody - middle region  
WB Suggested Anti-TRIM25 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Liver Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

TRIM25 / RNF147 antibody

  Rabbit Polyclonal Anti-TRIM25 Antibody - middle region  
WB Suggested Anti-TRIM25 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: RPMI 8226 cell lysate Rabbit IgG Purified Bov, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

TRIM25 / RNF147 antibody

  Rabbit Polyclonal Anti-TRIM25 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (TRIM25 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TRIM25 / RNF147 antibody

Western blot (WB) analysis of EFP antibody (Cat.-No.: AP06732PU-N) in extracts from K562 cells. Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TRIM25 / RNF147 antibody

Brain, cortex: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TRIM25 / RNF147 antibody

Western blot analysis of extracts from K562 cells, using ZNF147 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €230.00
  Assay Biotech

TRIM25 / RNF147 antibody

Western blot analysis of extracts from K562 cells, using ZNF147 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

TRIM25 - human gene knockout kit via CRISPR

TRIM25 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

TRIM25 - human gene knockout kit via CRISPR

TRIM25 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

TRIM25 - human gene knockout kit via CRISPR

TRIM25 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

TRIM25 - human gene knockout kit via CRISPR

TRIM25 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TRIM25 / RNF147

  Recombinant protein of human tripartite motif-containing 25 (TRIM25)  
TRIM25 / RNF147 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

TRIM25 overexpression lysate

TRIM25 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, TRIM25 (mGFP-tagged) - Human tripartite motif containing 25 (TRIM25), 200 uL, >10^7 TU/mL

Lenti ORF particles, TRIM25 (mGFP-tagged) - Human tripartite motif containing 25 (TRIM25), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, TRIM25 (Myc-DDK tagged) - Human tripartite motif containing 25 (TRIM25) , 200 uL, >10^7 TU/mL

Lenti ORF particles, TRIM25 (Myc-DDK tagged) - Human tripartite motif containing 25 (TRIM25) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

20 Item(s)

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