TRIM5 / RNF88 antibody

Principal name

TRIM5 / RNF88 antibody

Alternative names for TRIM5 / RNF88 antibody

RING finger protein 88, Tripartite motif-containing protein 5

SwissProt ID

Q0PF16 (Macmu), Q9C035 (Human)

Gene ID

85363 (TRIM5), 574288

Available reactivities

Hu (Human), Eq (Equine), Mky (Monkey), Prim (Primate), Rhesus Mky (Rhesus Monkey)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TRIM5 / RNF88 antibody

TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the a variant which also contains a carboxy-terminal B30.2 (SPRY) domain (1). Expression of TRIM5a variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1 (2,3), albeit at differing efficiencies. All TRIM5a variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5a acts as a natural barrier to cross-species retrovirus transmission (3). While a function has not yet been assigned to TRIM5g, it is known that expression of TRIM5a variants from humans, rhesus monkeys, and African green monkeys enable resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. Furthermore, the TRIM5d isoform appears to serve as a scaffold for the assembly of endogenous BTBD1 and BTBD2 proteins and also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner. The TRIM5d isoform has been shown to colocalize with the topoisomerase I-interacting proteins BTBD1 and BTBD2 in punctate or elongated cytoplasmic bodies in several mouse and human cells where it appears to serve as a scaffold for the assembly of endogenous BTBD proteins. TRIM5d also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner.

General readings

1. Ohkura S, Yap MW, Sheldon T, Stoye JP.
All three variable regions of the TRIM5alpha B30.2 domain can contribute to the specificity of retrovirus restriction. J Virol. 2006 Sep;80(17):8554-65. PMID: 16912305
2. Yap MW, Mortuza GB, Taylor IA, Stoye JP.
The design of artificial retroviral restriction factors. Virology. 2007 Sep 1;365(2):302-14.
PMID: 17493656
3. Yap MW, Lindemann D, Stanke N, Reh J, Westphal D, Hanenberg H, Ohkura S, Stoye JP. Restriction of foamy viruses by primate Trim5alpha. J Virol. 2008 Jun;82(11):5429-39. PMID: 18367529

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TRIM5 / RNF88 antibody

  Rabbit Polyclonal Anti-TRIM5 Antibody  
Hum. Adult Placenta; Host: Rabbit; Target Name: CHAD; Sample Tissue: Human Adult Placenta; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

TRIM5 / RNF88 (N-term) antibody

  Goat Polyclonal Antibody against TRIM5  
TA302865 staining (1µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRIM5 / RNF88 (Isoform gamma) (C-term) antibody

  Rabbit Polyclonal TRIM5 gamma Antibody  
Western blot analysis of TRIM5 gamma expression in human bladder (A) and colon (B) cell lysate with TRIM5 gamma antibody at 2 ug /ml. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRIM5 / RNF88 (Isoform delta) antibody

  Rabbit Polyclonal TRIM5 delta Antibody  
Western blot analysis of TRIM5 delta expression in mouse brain cell lysate with TRIM5 delta antibody at 2 ug/ml. Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRIM5 / RNF88 (Isoform alpha) antibody

  Rabbit Polyclonal TRIM5 alpha Antibody  
Western blot analysis of TRIM5 alpha expression in human stomach (A), thymus (B), and uterus (C) cell lysate with TRIM5 alpha antibody at 2 ug /ml. Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRIM5 / RNF88 (Isoform alpha) (C-term) antibody

  Rabbit Polyclonal TRIM5 alpha Antibody  
Western blot analysis of TRIM5 alpha expression in human stomach (A), thymus (B), and uterus (C) cell lysate with TRIM5 alpha antibody at 2 ug /ml. Rabbit IgG Aff - Purified Hu, Rhesus Mky E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRIM5 / RNF88 (Isoform alpha) antibody

  Rabbit Polyclonal TRIM5 alpha Antibody  
Western blot analysis of TRIM5 alpha expression in human stomach (A), thymus (B), and uterus (C) cell lysate with TRIM5 alpha antibody at 2 ug /ml. Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRIM5 / RNF88 antibody

Figure 1. Staining TRIM5 in Brain, cortex by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Aff - Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TRIM5 / RNF88 (2-15) antibody

Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Hu, Mky E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TRIM5 / RNF88 (C-term) antibody

Human Small Intestine (formalin-fixed, paraffin-embedded) stained with TRIM5 antibody AP22859PU-N at 2,5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TRIM5 / RNF88 antibody

IHC Image for AP22936PU-N
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Mky P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

TRIM5 - human gene knockout kit via CRISPR

TRIM5 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

TRIM5 - human gene knockout kit via CRISPR

TRIM5 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

TRIM5 - human gene knockout kit via CRISPR

TRIM5 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

TRIM5 - human gene knockout kit via CRISPR

TRIM5 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

TRIM5 overexpression lysate

TRIM5 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

TRIM5 overexpression lysate

TRIM5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

TRIM5 overexpression lysate

TRIM5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

TRIM5 overexpression lysate

TRIM5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

TRIM5 overexpression lysate

TRIM5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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