TRIM54 / MURF3 / RNF30 antibody

Principal name

TRIM54 / MURF3 / RNF30 antibody

Alternative names for TRIM54 / MURF3 / RNF30 antibody

MURF-3, MuRF, RING finger protein 30, Tripartite motif-containing protein 54, Muscle-specific RING finger protein 3

SwissProt ID

Q58D15 (Bovin), Q5XIH6 (Rat), Q9BYV2 (Human), Q9ERP3 (Mouse)

Gene ID

57159 (TRIM54)

Available reactivities

Hu (Human), Can (Canine), Bov (Bovine), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of TRIM54 / MURF3 / RNF30 antibody

MuRF1, is a nuclear protein that interacts with SMT3b and the large myofibrillar protein Titin. In muscle cells, MuRF2 (RFN29) regulates gene expression and protein turnover. It localizes to the cytoplasm, but under atrophic conditions it is detected in the nucleus. MuRF2 can form oligomers with various other proteins, including Titin and Myosin. MuRF3, also designated tripartite motif-containing 54 (TRIM54) or ring finger protein 30 (RNF30), interacts with tubulin and stabilizes microtubules duing myotube formation. It is a cytoplasmic protein the localizes to the Z-lines in skeletal muscles, while MuRF2 localizes to the sarcomeric M-band in cardiomyocytes. MuRF3 shares 77% and 65% sequence identity with MuRF1 and MuRF2, respectively. MuRF1-3 share a conserved N-terminal RING domain and zinc-binding B-box motif, and two coiled-coil dimerization motif boxes, in their central regions.

General readings

Centner T, Yano J, Kimura E, McElhinny AS, Pelin K, Witt CC, Bang ML, Trombitas K, Granzier H, Gregorio CC, Sorimachi H, Labeit S.
Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain.
J Mol Biol. 2001 Mar 2;306(4):717-26.
PMID: 11243782

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TRIM54 / MURF3 / RNF30 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRIM54 mouse monoclonal antibody. (TA803871) Dilution: 1:150 Mouse IgG1 OTI7F8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRIM54 / MURF3 / RNF30 (C-term) antibody

TA302860 staining (1ug/ml) of human muscle lysate (RIPA buffer, 35ug total\nprotein per lane).  Primary incubated for 1 hour.  Detected by western blot\nusing chemiluminescence. Goat Aff - Purified Can, Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRIM54 / MURF3 / RNF30 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM54 (RC218075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM54. Mouse IgG1 OTI6F5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRIM54 / MURF3 / RNF30 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM54 (RC218075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM54. Mouse IgG1 OTI4E7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRIM54 / MURF3 / RNF30 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM54 (RC218075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM54. Mouse IgG1 OTI6A9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRIM54 / MURF3 / RNF30 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM54 (RC218075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM54. Mouse IgG1 OTI5D9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRIM54 / MURF3 / RNF30 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM54 (RC218075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM54. Mouse IgG1 OTI2C7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRIM54 / MURF3 / RNF30 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TRIM54 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803872) Mouse IgG1 OTI5A11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRIM54 / MURF3 / RNF30 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM54 (RC218075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM54. Mouse IgG1 OTI6C3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRIM54 / MURF3 / RNF30 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM54 (RC218075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM54. Mouse IgG1 OTI2A11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRIM54 / MURF3 / RNF30 antibody

TRIM54 / MURF3 / RNF30 Mouse IgG2b 2G9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TRIM54 / MURF3 / RNF30 antibody

TRIM54 polyclonal antibody ( Cat # PAB6356 ) staining ( 1ug/ml ) of human muscle lysate ( RIPA buffer, 35ug total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

TRIM54 / MURF3 / RNF30 antibody

TRIM54 / MURF3 / RNF30 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

TRIM54 / MURF3 / RNF30 antibody

Human HeLa; WB Suggested Anti-TRIM54 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate; TRIM54 antibody - N-terminal region (ARP43486_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

TRIM54 / MURF3 / RNF30 antibody

NB 100-1197 staining (1ug/ml) of Human Muscle lysate (RIPA buffer, 35ug total\nprotein per lane). Primary incubated for 1 hour. Detected by western blot\nusing chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

TRIM54 / MURF3 / RNF30 antibody

Western Blot: MURF3 Antibody [NBP1-52899] - This Anti-TRIM54 antibody was used in Western Blot of Hela tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

TRIM54 / MURF3 / RNF30 antibody

Immunohistochemistry-Paraffin: MURF3 Antibody [NBP1-92154] - Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TRIM54 / MURF3 / RNF30 (N-term HIS tag, transcript variant 1)

TRIM54 / MURF3 / RNF30 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

TRIM54 / MURF3 / RNF30

TRIM54 / MURF3 / RNF30 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

TRIM54 / MURF3 / RNF30

TRIM54 / MURF3 / RNF30 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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