TRIM54 / MURF3 / RNF30 antibody

Principal name

TRIM54 / MURF3 / RNF30 antibody

Alternative names for TRIM54 / MURF3 / RNF30 antibody

MURF-3, MuRF, RING finger protein 30, Tripartite motif-containing protein 54, Muscle-specific RING finger protein 3

SwissProt ID

Q58D15 (Bovin), Q5XIH6 (Rat), Q9BYV2 (Human), Q9ERP3 (Mouse)

Gene ID

57159 (TRIM54), 58522 (Trim54), 362708

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of TRIM54 / MURF3 / RNF30 antibody

Kit Component:
- KN218075G1, TRIM54 gRNA vector 1 in pCas-Guide vector
- KN218075G2, TRIM54 gRNA vector 2 in pCas-Guide vector
- KN218075D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Centner T, Yano J, Kimura E, McElhinny AS, Pelin K, Witt CC, Bang ML, Trombitas K, Granzier H, Gregorio CC, Sorimachi H, Labeit S.
Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain.
J Mol Biol. 2001 Mar 2;306(4):717-26.
PMID: 11243782

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TRIM54 / MURF3 / RNF30 antibody

  TRIM54 mouse monoclonal antibody, clone OTI7F8 (formerly 7F8)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRIM54 mouse monoclonal antibody. (TA803871) Dilution: 1:150 Mouse IgG1 OTI7F8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRIM54 / MURF3 / RNF30 (C-term) antibody

  Goat Polyclonal Antibody against TRIM54  
TA302860 staining (1ug/ml) of human muscle lysate (RIPA buffer, 35ug total\nprotein per lane).  Primary incubated for 1 hour.  Detected by western blot\nusing chemiluminescence. Goat Aff - Purified Can, Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRIM54 / MURF3 / RNF30 antibody

  TRIM54 mouse monoclonal antibody, clone OTI6F5 (formerly 6F5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM54 (RC218075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM54. Mouse IgG1 OTI6F5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRIM54 / MURF3 / RNF30 antibody

  TRIM54 mouse monoclonal antibody, clone OTI4E7 (formerly 4E7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM54 (RC218075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM54. Mouse IgG1 OTI4E7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRIM54 / MURF3 / RNF30 antibody

  TRIM54 mouse monoclonal antibody, clone OTI6A9 (formerly 6A9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM54 (RC218075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM54. Mouse IgG1 OTI6A9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRIM54 / MURF3 / RNF30 antibody

  TRIM54 mouse monoclonal antibody, clone OTI5D9 (formerly 5D9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM54 (RC218075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM54. Mouse IgG1 OTI5D9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRIM54 / MURF3 / RNF30 antibody

  TRIM54 mouse monoclonal antibody, clone OTI2C7 (formerly 2C7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM54 (RC218075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM54. Mouse IgG1 OTI2C7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRIM54 / MURF3 / RNF30 antibody

  Purified TRIM54 mouse monoclonal antibody, clone OTI5A11 (formerly 5A11)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TRIM54 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803872) Mouse IgG1 OTI5A11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRIM54 / MURF3 / RNF30 antibody

  TRIM54 mouse monoclonal antibody, clone OTI6C3 (formerly 6C3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM54 (RC218075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM54. Mouse IgG1 OTI6C3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRIM54 / MURF3 / RNF30 antibody

  TRIM54 mouse monoclonal antibody, clone OTI2A11 (formerly 2A11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM54 (RC218075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM54. Mouse IgG1 OTI2A11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TRIM54 / MURF3 / RNF30 antibody

TRIM54 / MURF3 / RNF30 Mouse IgG2b 2G9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TRIM54 / MURF3 / RNF30 antibody

TRIM54 polyclonal antibody ( Cat # PAB6356 ) staining ( 1ug/ml ) of human muscle lysate ( RIPA buffer, 35ug total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

TRIM54 / MURF3 / RNF30 antibody

TRIM54 / MURF3 / RNF30 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

TRIM54 / MURF3 / RNF30 antibody

Human HeLa; WB Suggested Anti-TRIM54 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate; TRIM54 antibody - N-terminal region (ARP43486_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

TRIM54 - human gene knockout kit via CRISPR

TRIM54 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

TRIM54 - human gene knockout kit via CRISPR

TRIM54 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

TRIM54 - human gene knockout kit via CRISPR

TRIM54 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

TRIM54 - human gene knockout kit via CRISPR

TRIM54 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TRIM54 / MURF3 / RNF30 (N-term HIS tag, transcript variant 1)

  Purified recombinant protein of Human tripartite motif containing 54 (TRIM54), transcript variant 1, with N-terminal HIS tag, expressed in E.Coli, 50ug  
TRIM54 / MURF3 / RNF30 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

TRIM54 / MURF3 / RNF30

TRIM54 / MURF3 / RNF30 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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