TrkB antibody

Principal name

TrkB antibody

Alternative names for TrkB antibody

TRKB, NTRK2, BDNF/NT-3 growth factors receptor, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, GP145-TrkB

SwissProt ID

P15209 (Mouse), Q16620 (Human), Q63604 (Rat)

Gene ID

4915 (NTRK2), 18212 (Ntrk2), 25054

Available reactivities

Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse, Rat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of TrkB antibody

BDNF and NT-4 belong to the neurotrophin family which also includes NGF and NT-3. These neurotrophins bind two groups of receptors. The p75NTR receptor is common to all four neurotrophins and is a member of the tumor necrosis factor receptor family. The tropomyosin-related kise (Trk) receptors are receptor tyrosine kises (RTKs) and three receptors form this family: TrkA, TrkB, and TrkC.As mentioned above, the p75NTR receptor binds to all neurotrophins with similar affinities while the Trk receptors are the ones to display the selectivity for the neurotrophins. TrkA is activated by NGF binding, TrkB by that of BDNF and NT-4, while TrkC is stimulated by the binding of NT-3.All three Trk receptors are highly expressed in the mammalian brain in very distinct regions and are also expressed in the peripheral nervous system.Cholinergic neurons in the basal forebrain exclusively express TrkA, while TrkB and TrkC are highly expressed in the hippocampus. Motor and sensory neurons in the peripheral nervous system express Trk receptors. Interestingly, Trk receptors are not essential for development, but knockout mice die shortly after birth. Indeed, TrkB-deficient mice demonstrate a significant decrease in motor neurons and syptogenesis.Trk receptors have many motifs in the extracellular region, including cell-adhesion domains, three tandem leucine rich motifs flanked by two clusters of cysteines. In the membrane proximal region of the receptor there are also two immunoglobulin-like domains. The binding of neurotrophins to Trk receptors promotes receptor dimerization resulting in kise activation. Activated Trk receptors then phosphorylate a cascade of sigling molecules including the Ras/ERK, PI3K/Akt pathways and PLC-g1. Activated Trk receptors also create interl docking sites for other sigling adaptor proteins to bind to. Splice variants of TrkA, TrkB and TrkC have been observed. These splice isoforms are mainly affected in the tyrosine kise domain of the receptor lying in the cytoplasm. Endocytosis is an important sigling trait of Trk receptors.Following neurotrophin binding to the Trk receptor, the receptor complex is then interlized via endocytosis in order to termite sigling. However, in the axol compartment of neurons the interlization process of the neurotrophin complexed to the receptor is part of the sigling process and is important for activating transcription processes in the nucleus.

General readings

Heldt SA et al., (2014) J. Neurosci., 34:(7)2444-2450.Xiang J et al., (2014) J. Clin. Invest., 124:(1)85-98.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TrkB (1-429) antibody

Immunohistochemistry: AP08871PU-N TrkB antibody staining of Formalin-Fixed Paraffin-Embedded of Human Brain, Cortex at 10 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €425.00
  OriGene Technologies GmbH

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