TRPC3 / TRP3 antibody
Principal name
TRPC3 / TRP3 antibody
Alternative names for TRPC3 / TRP3 antibody
Transient receptor protein 3, TRP-3, Short transient receptor potential channel 3
Ncbi ID
9606, NP_001124170.1, NP_003296, 10090, NM_003305, CAA74083, NM_001130698
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of TRPC3 / TRP3 antibody
TRPC3(Transient receptor potential cation channel, subfamily C, member 3) also known as TRP3, is expressed in megakaryocytic cell lines and therefore may play a role in calcium homeostasis in megakaryocytes and platelets. Using patch-clamp and whole-cell recordings of human embryonic kidney cells transfected with TRPC3, TRPC3 behaves as an inositol phosphate and diacylglycerol-gated channel, but not as a store-operated channel. The TRPC3 gene, but not other TRPC genes, was damaged in several mutant T-cell lines with defective calcium influx, resulting in expression of truncated TRPC3 transcripts. Reduced calcium channel activity could be reversed by restoration of wildtype TRPC3. TRPC3 is part of endogenous calcium channels in T lymphocytes and contributes to T-cell receptor-dependent calcium entry into T cells. Neuropathologic studies showed impaired growth and differentiation of dendritic arbors of the Purkinje cells, suggesting that normal TRPC3 plays a role in the development and survival of cerebellar Purkinje cells.
Spleen: Formalin-Fixed Paraffin-Embedded (FFPE)
Spleen: Formalin-Fixed Paraffin-Embedded (FFPE)
Immunohistochemistry of TRPC3 in mouse heart tissue with TRPC3 polyclonal antibody ( Cat # PAB13219 ) at 10 µg/ml.
Western blot analysis of TRPC3 in mouse heart tissue lysate with TRPC3 polyclonal antibody ( Cat # PAB13219 ) at ( A ) 1 and ( B ) 2 µg/ml.
Immunohistochemistry of TRPC3 in mouse heart tissue with TRPC3 polyclonal antibody ( Cat # PAB13224 ) at 2 µg/ml.
Western blot analysis of TRPC3 in human brain tissue lysate with TRPC3 polyclonal antibody ( Cat # PAB13224 ) at ( A ) 1 and ( B ) 2 µg/ml.
Mouse brain; TRPC3 antibody - N-terminal region (ARP58715_P050) validated by WB using brainstem , Cerebellum, Thalamus, Hipppcampus, Cerebral Cortex; TRPC3 antibody - N-terminal region (ARP58715_P050) in Mouse brain cells using Western Blot
Human HepG2; WB Suggested Anti-TRPC3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; TRPC3 antibody - N-terminal region (ARP58715_P050) in Human HepG2 cells using Western Blot
Western blot analysis of TRPC3 in mouse heart tissue lysate with TRPC3 antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry of TRPC3 in mouse heart tissue with TRPC3 antibody at 10 ug/ml.
Immunohistochemistry of TRPC3 in mouse heart tissue with AP30955PU-N at 2 μg/ml.
Western blot analysis of TRPC3 in human brain tissue lysate with AP30955PU-N at (A) 1 and (B) 2 μg/ml.
Predicted cell location: Cytoplasm. Positive control: Human thyroid and liver cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using TRPC3 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human thyroid and liver cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using TRPC3 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted band size: 97 kDa. Positive control: Human fetal brain tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/1600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes)
Predicted cell location: Cytoplasm or Nucleus. Positive control: Human lung cancer tissue. Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using TRPC3 antibody at dilution 1/80, on the right is treated with the synthetic peptide. (Original magnification:x200)
Western blot analysis of rat heart membranes: 1. Anti-TRPC3 antibody, (1:200). 2. Anti-TRPC3 antibody, preincubated with the control peptide antigen.
Expression of TRPC3 in rat pituitary gland. Immunohistochemical staining of rat pituitary gland paraffin embedded sections using Anti-TRPC3 antibody, (1:100). TRPC3 is mainly expressed in the adenohypophysis (on left). Hematoxilin is used as the counterstain.
Expression of TRPC3 in mouse cerebellum. Immunohistochemical staining of mouse cerebellum using Anti-TRPC3 antibody (A). Immunoreactivity appears in the molecular layer and in Purkinje cells (B).
IHC staining of TRPC3 in rat cerebellum using Anti-TRPC3 antibody. A. TRPC3 (green) appears in Purkinje cells (arrows) including both soma and dendrites and as well as in the molecular layer neuropil (asterisk). Staining of the same section with mouse anti-parvalbumin (red) reveals that TRPC3 is not expressed in molecular layer interneurons. B. Pre-incubating Anti-TRPC3 with the TRPC3 peptide antigen blocks staining. DAPI is used as the counterstain (blue).
Colocalization of TRPC6 and TRPC3 in rat cerebellum. Immunohistochemical staining of rat cerebellum frozen section using guinea pig Anti-TRPC6 antibody and rabbit Anti-TRPC3 antibody. A. TRPC6 staining (green) appears in molecular layer and in Purkinje cells. B. In the same section, staining of TRPC3 (red) appears as well in both molecular layer and Purkinje cells. C. Merge images of A and B indicates extensive co-localization. DAPI is used as the counterstain (blue).
Expression of TRPC3 in rat C6 brain glioma cells. Immunocytochemical staining of Paraformaldehyde-fixed and permeabilized rat C6 brain glioma cells. A. Staining using Anti-TRPC3 antibody, (1:500) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. B. Nuclear staining using the cell-permeable dye Hoechst 33342. C. Merged image of panels A and B.
WB Suggested Anti-TRPC3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
TRPC3 antibody - N-terminal region validated by WB using brainstem , Cerebellum, Thalamus, Hipppcampus, Cerebral Cortex
Western blot analysis of TRPC3 in human cerebellum tissue lysate with TRPC3 antibody at (A) 1 and (B) 2 ug/ml.
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- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA328732 | TRPC3 / TRP3 antibody |
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| Rabbit Polyclonal Anti-TRPC3 | |||||||
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Rabbit | Purified | Hu, Ms, Rt | WB |
0.2 ml /
€585.00
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| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody! |
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