TRPC4 / TRP4 antibody

Principal name

TRPC4 / TRP4 antibody

Alternative names for TRPC4 / TRP4 antibody

Trp-related protein 4, TRP-4, CCE1, Short transient receptor potential channel 4, Capacitative calcium entry channel 1

SwissProt ID

O35119 (Rat), P79100 (Bovin), Q9QUQ5 (Mouse), Q9UBN4 (Human)

Gene ID

7223 (TRPC4), 22066 (Trpc4), 84494

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Sheep, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of TRPC4 / TRP4 antibody

Cytosolic Ca2+ serves as an intracellular mediator for many extracellular signals. At rest, cells maintain a low Ca2+ concentration of ~ 10-7 M. Upon activation of the phospholipase C-dependent mechanism, the cytosolic Ca2+ concentration rises. In many vertebrate and invertebrate cells, the influx of Ca2+ is biphasic. Mobilization of Ca2+ from internal stores (sarcoplasmic reticulum in muscle cells and endoplasmic reticulum in other cell types) drives the initial burst. The second phase, referred to as capacitative Ca2+ entry (CCE) or store-operated Ca2+ entry, occurs when the depletion of intracellular Ca2+ stores activates a non-voltagesensitive plasma membrane Ca2+ conductance.1 The channels responsible for this conductance have been referred to as SOCs for store-operated channels. A Drosophila gene, trp (transient receptor potential), encodes the first identified candidate for such a channel. In recent years, seven mammalian TRP channels, named TRPC1-TRPC7, have been cloned. TRP channels (TRPCs) are ubiquitous, yet are most abundant in muscles and nerves. They differ in their method of activation and in their channel conductance.2 All TRPCs have six transmembrane segments with a pore-forming loop between the fifth and sixth segment. This structure is similar to the pore-forming subunits of other channels including voltage-gated Na+, K+ and Ca2+ channels and cyclic nucleotide gated channels. The TRP isoforms can be divided into three subfamilies based on characteristic sequence domains. The long TRP channels (LTRPCs) have four related sequences with open reading frames (ORFs) coding for approximately 1600 amino acids. The other two subfamilies are characterized by shorter ORFs, coding for about 900 amino acids. These are called the short TRP (STRP) and osm-9-like (OTRP) channel families. The OTRPCs are named after the first member of this family to be identified, the C. elegans clone osm-9.2 The sub-families differ in other ways. STRPCs have two to four ankyrin domains in their N-terminal cytosolic tail, where as OTRPCs have three or more and LTRPCs have none. Furthermore, a proline-rich motif in the cytosolic C-terminal region near the sixth transmembrane segment can be found in STRPCs and LTRPCs, but not in OTRPCs.2 The sub-families also differ in their functional properties. The seven mammalian TRPCs (TRPCs 1-7) are all members of the STRPC sub-family. STRPCs are activated in response to phospholipase C activation. OTRPCs appear to be activated by physical or chemical stimuli such as heat, osmotic stress or mechanical stress. The function and activation of LTRPCs is not yet known since their ability to function as channels has not been demonstrated. TRPC4 has been identified in adrenal gland, testis and brain.2 Full-length cDNAs have been cloned from bovine, human, mouse and rat species. TRPC4 is a non-selective cation channel that integrates signaling pathways from G-protein-coupled receptors and receptor tyrosine kinases independently of the depletion of calcium stores.3

General readings

Song X, Zhao Y, Narcisse L, Duffy H, Kress Y, Lee S, Brosnan CF.
Canonical transient receptor potential channel 4 (TRPC4) co-localizes with the scaffolding protein ZO-1 in human fetal astrocytes in culture.
Glia. 2005 Feb;49(3):418-29
PMID: 15540229

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  2. Highly Validated (2)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TRPC4 / TRP4 antibody

Western blot analysis of rat brain membranes: 1. Anti-TRPC4 antibody, (1:200). 2. Anti-TRPC4 antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

TRPC4 / TRP4 antibody

Immunohistochemistry-Paraffin: TRPC4 Antibody [NB110-74765] - IHC on rat brain, paraffin sections using Rabbit antibody to 1t cytoplasmic loop of TRPC4 (TRP4): whole serum at 1 : 500 dilution incubated overnight at 4C and developed with DAB/Ni. Rabbit Serum Ms, Rt C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

TRPC4 / TRP4 antibody

TA305678 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRPC4 / TRP4 antibody

IHC analysis of TrpC4 in human hippocampal tissue using anti-trpc4. Mouse IgG2b S77-15 Purified Hu, Ms, Rt C, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TRPC4 / TRP4 antibody

Western blot analysis of rat and mouse brain lysates: 1. Anti-TRPC4 (extracellular) antibody, (1:200). 2. Anti-TRPC4 (extracellular) antibody, preincubated with the control antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

TRPC4 / TRP4 antibody

WB Suggested Anti-TRPC4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

TRPC4 / TRP4 antibody

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with TrpC4 and probed with N77/15 TC supe. Mouse IgG2b N77/15 Supernatant Hu, Ms, Rt ICC/IF, IP, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

TRPC4 / TRP4 antibody

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with TrpC4 and probed with N77/15 TC supe. Mouse IgG2b N77/15 Purified Hu, Ms, Rt ICC/IF, IP, WB
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

TRPC4 / TRP4 (C-term) antibody

TRPC4 Antibody (C-term) (AP54360PU-N) western blot analysis in NCI-H292 cell line lysates (35 µg/lane). This demonstrates the TRPC4 antibody detected the TRPC4 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TRPC4 / TRP4 antibody

TRPC4 polyclonal antibody ( Cat # PAB11598 ) ( 0.3 µg/ml ) staining of rat brain lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

TRPC4 / TRP4 antibody

Human HepG2; Host: Rabbit. Target Name: TRPC4. Sample Tissue: HepG2. Antibody Dilution: 1.0ug/ml.; TRPC4 antibody - middle region (ARP35256_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

TRPC4 / TRP4 (930-947) antibody

TRPC4 staining of adult rat hippocampus. Mouse IgG2b Supernatant Hu, Ms, Rt C, IP, WB
0.1 ml / €440.00
  Neuromics Antibodies

TRPC4 / TRP4 antibody

TRPC4 / TRP4 Sheep Serum Rt C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

TRPC4 / TRP4 antibody

TRPC4 / TRP4 Rabbit IgG Aff - Purified Ms, Rt C, P, WB
50 µl / €380.00
  Novus Biologicals Inc.

TRPC4 / TRP4 antibody

Western Blot: TRPC4 Antibody [NBP1-20989] - (0.3µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer) Goat IgG Aff - Purified Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

15 Item(s)

per page
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