Bov (Bovine), Can (Canine), Chk (Chicken), Dros (Drosophila), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ye (Yeast)
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of TRSPAP1 / TRNAU1AP antibody
TRSPAP1 is involved in the early steps of selenocysteine biosynthesis and tRNA(Sec) charging to the later steps resulting in the cotranslational incorporation of selenocysteine into selenoproteins. It stabilizes the SECISBP2, EEFSEC and tRNA(Sec) complex. It may be involved in the methylation of tRNA(Sec) and enhances efficiency of selenoproteins synthesis.
van (2006) Mol. Cell. Biol. 26 (6), 2337-2346
Human Jurkat; WB Suggested Anti-TRSPAP1 Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysate. TRNAU1AP is supported by BioGPS gene expression data to be expressed in Jurkat.; TRSPAP1 antibody - middle region (ARP40880_T100) in Human Jurkat cells using Western Blot
Human Intestine; WB Suggested Anti-TRSPAP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Intestine; TRSPAP1 antibody - N-terminal region (ARP40881_P050) in Human Intestine cells using Western Blot
Western Blot: TRSPAP1 Antibody [NBP1-92536] Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: Negative control (vector only transfected HEK293T lysate) | Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.