Trypsin Inhibitor antibody

Principal name

Trypsin Inhibitor antibody

Gene ID

547831 (KTI3)

Ncbi ID

3847

Available reactivities

Lima Bean, Soy Bean, Pl (Plant)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C)

Background of Trypsin Inhibitor antibody

Trypsin is a 25 kDa enzyme of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, but the active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin prefers lysine and arginine residues. One of the substrates for trypsin is chymotrypsinogen which is cleaved to produce active chymotrypsin. High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screen for and diagnose Cystic Fibrosis. The soybean trypsin inhibitor was first crystallized by Kunitz in 1945 and is one of several such inhibitors found in soybeans. It inhibits the proteolytic action approximately of an equal weight of crystalline trypsin by combining with trypsin to form a stable compound. Chymotrypsin is only slightly inhibited by soy inhibitor.

9 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Trypsin Inhibitor antibody

Trypsin Inhibitor Rabbit Serum Lima Bean E, IP, WB
2 ml / €1,150.00
  Acris Antibodies GmbH

Trypsin Inhibitor antibody

Trypsin Inhibitor Rabbit Biotin Lima Bean E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Trypsin Inhibitor antibody

Trypsin Inhibitor Rabbit HRP Lima Bean E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Trypsin Inhibitor antibody

Trypsin Inhibitor Rabbit Ig Fraction Lima Bean E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Trypsin Inhibitor antibody

Trypsin Inhibitor Rabbit Serum Soy Bean E, IP, WB
2 ml / €1,150.00
  Acris Antibodies GmbH

Trypsin Inhibitor antibody

Biotin conjugated rabbit anti-trypsin inhibitor antibody Cat.-No. R1115B (lot 6594) and rabbit anti streptavidin Cat.-No. AP09170PU-N were used to detect target proteins Trypsin inhibitor (left) and streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1µg of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1/1000 dilution of primary antibody (4° C). Detection shown was using Dylight 649 conjugated donkey anti rabbit secondary antibody and imaged on the BioRad VersaDoc System. Rabbit Biotin Soy Bean E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Trypsin Inhibitor antibody

Trypsin Inhibitor Rabbit HRP Soy Bean C, E, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Trypsin Inhibitor antibody

Rabbit anti soybean Trypsin inhibitor antibody Cat.-No. R1115P (Lot 6594) was used to detect purified soybean Trypsin inhibitor. Samples of ~1 and 0.25 µg of purified soybean Trypsin inhibitor per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of protein contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with rabbit anti soybean Trypsin inhibitor antibody (1/5,000 overnight on 4° C). Primary antibody was detected with Dylight 488 conjugated streptavidin  and Atto 425 conjugated goat anti rabbit secondary antibody. Predicted/observed size: 24 kDa, 17 kDa for Trypsin. Other band(s): none. Rabbit Ig Fraction Soy Bean E, IP, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Trypsin Inhibitor antibody

Trypsin Inhibitor Rabbit IgG Aff - Purified Pl E, WB
0.5 ml / €360.00
  Novus Biologicals Inc.

9 Item(s)

per page
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