TSC1 / Hamartin antibody

Principal name

TSC1 / Hamartin antibody

Alternative names for TSC1 / Hamartin antibody

KIAA0243, TSC, Tuberous sclerosis 1 protein

SwissProt ID

Q92574 (Human), Q9Z136 (Rat)

Gene ID

7248 (TSC1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Dot blot (Dot)

Background of TSC1 / Hamartin antibody

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1,2). TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high (3). Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro (4), suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth (4,5). A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.

General readings

Chan JA, Zhang H, Roberts PS, Jozwiak S, Wieslawa G, Lewin-Kowalik J, Kotulska K, Kwiatkowski DJ. Pathogenesis of tuberous sclerosis subependymal giant cell astrocytomas: biallelic inactivation of TSC1 or TSC2 leads to mTOR activation. J Neuropathol Exp Neurol. 2004 Dec;63 (12):1236-42. PMID: 15624760 Other PMIDs: 15611338, 15647351

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TSC1 / Hamartin antibody

Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-TSC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801949) Mouse IgG2a OTI3A2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TSC1 / Hamartin antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TSC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801951) Mouse IgG2b OTI5A8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TSC1 / Hamartin (Isoform 1) antibody

TA302506 (0.1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TSC1 / Hamartin (C-term) antibody

Western blot analysis of TSC1 in C2C12 cell lysate with TSC1 antibody at (A) 2 and (B) 4 µg. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TSC1 / Hamartin (Center) antibody

Western blot analysis of TSC1 in EL4 cell lysate with TSC1 antibody at 1 ug/mL in the (A) absence and (B) presence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TSC1 / Hamartin antibody

Western blot analysis of TSC1 expression in HeLa cell lysate Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TSC1 / Hamartin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC1 (RC213332, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC1. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TSC1 / Hamartin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC1 (RC213332, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC1. Mouse IgG1 OTI2G3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TSC1 / Hamartin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC1 (RC213332, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC1. Mouse IgG2a OTI2B2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TSC1 / Hamartin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC1 (RC213332, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC1. Mouse IgG2a OTI5A7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TSC1 / Hamartin antibody

Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TSC1 / Hamartin antibody

Figure 2. Staining TSC1 in Heart by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TSC1 / Hamartin pSer505 antibody

Dot blot analysis of anti-TSC1-pSer505 Phospho-specific Pab (Cat#AP12886PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

TSC1 / Hamartin Control Peptide

TSC1 / Hamartin
50 µg / €160.00
  Acris Antibodies GmbH

TSC1 / Hamartin Control Peptide

TSC1 / Hamartin
50 µg / €160.00
  Acris Antibodies GmbH

TSC1 / Hamartin antibody

Immunocytochemical staining of EL4 cells with Rabbit anti Human TSC1 (SP2163P) Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

TSC1 / Hamartin antibody

Immunocytochemical staining of EL4 cells with Rabbit anti Human TSC1 (SP2163P) Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µg / €300.00
  Acris Antibodies GmbH

TSC1 / Hamartin antibody

TSC1 / Hamartin Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

TSC1 / Hamartin antibody

TSC1 / Hamartin Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

TSC1 / Hamartin antibody

HEK293 overexpressing human TSC1 and probed with TSC1 polyclonal antibody ( Cat # PAB7016 ) ( mock transfection in first lane ), tested by Origene. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

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