TSC1 / Hamartin antibody
Principal name
TSC1 / Hamartin antibody
Alternative names for TSC1 / Hamartin antibody
KIAA0243, TSC, Tuberous sclerosis 1 protein
Gene ID
7248 (TSC1)
Ncbi ID
Available reactivities
Available hosts
Available applications
Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Dot blot (Dot)
Background of TSC1 / Hamartin antibody
Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1,2). TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high (3). Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro (4), suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth (4,5). A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.
General readings
Chan JA, Zhang H, Roberts PS, Jozwiak S, Wieslawa G, Lewin-Kowalik J, Kotulska K, Kwiatkowski DJ. Pathogenesis of tuberous sclerosis subependymal giant cell astrocytomas: biallelic inactivation of TSC1 or TSC2 leads to mTOR activation. J Neuropathol Exp Neurol. 2004 Dec;63 (12):1236-42. PMID: 15624760 Other PMIDs: 15611338, 15647351
Western Blot and Immunoprecipitation: TSC1 Antibody [NB100-2315] - Detection of Human and Mouse TSC1 on HeLa whole cell lysate using NB100-2315.
Western Blot: Hamartin Antibody [NB100-2821] - NB100-2821 (0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: TSC1 Antibody [NB100-2821] - HEK293 overexpressing Human TSC1 (RC213332) and probed (mock transfection in first lane), tested by Origene.
Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE)
Heart: Formalin-Fixed Paraffin-Embedded (FFPE)
Figure 2. Staining TSC1 in Heart by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
Figure 1. Staining TSC1 in Skeletal Muscle by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
HEK293 overexpressing human TSC1 and probed with TSC1 polyclonal antibody ( Cat # PAB7016 ) ( mock transfection in first lane ), tested by Origene.
TSC1 polyclonal antibody ( Cat # PAB7016 ) ( 0.1 µg/ml ) staining of human brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Dot blot analysis of anti-TSC1-pSer505 Phospho-specific Pab (Cat#AP12886PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
HEK293 overexpressing Human TSC1 and probed with AP16481PU-N (mock transfection in first lane), tested by Origene.
AP16481PU-N (3.8µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
AP16481PU-N (0.1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of TSC1 in C2C12 cell lysate with TSC1 polyclonal antibody ( Cat # PAB13144 ) at ( A ) 2 and ( B ) 4 µg.
Immunocytochemistry of TSC1 in EL4 cells with TSC1 polyclonal antibody ( Cat # PAB13145 ) at 2 µg/ml
Western blot analysis of TSC1 in EL4 cell lysate with TSC1 polyclonal antibody ( Cat # PAB13145 ) at 1 µg/ml in the ( A ) absence and ( B ) presence of blocking peptide.
Dot blot analysis of TSC1 (phospho S505) polyclonal antibody ( Cat # PAB8143 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml.
Western Blot: TSC1 Lysate [NBL1-17350] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for TSC1
ELISA: TSC1 Antibody [24920002]
Western blot analysis of TSC1 in C2C12 cell lysate with AP30959PU-N at (A) 2 and (B) 4 μg.
Immunocytochemistry of TSC1 in EL4 cells with AP30960PU-N at 2 μg/ml
Western blot analysis of TSC1 in EL4 cell lysate with AP30960PU-N at 1 μg/ml in the (A) absence and (B) presence of blocking peptide.
Immunohistochemical of paraffin-embedded human kidney using 20988-1-AP(hamartin antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human skeletal muscle using 20988-1-AP(hamartin antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skeletal muscle using 20988-1-AP(hamartin antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human kidney using 20988-1-AP(hamartin antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HeLa cells using 20988-1-AP( hamartin Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
HeLa cells were subjected to SDS PAGE followed by western blot with 20988-1-AP(hamartin antibody) at dilution of 1:1000
HeLa cells were subjected to SDS PAGE followed by western blot with 20988-1-AP(hamartin antibody) at dilution of 1:1000
IP Result of anti-hamartin (IP:20988-1-AP, 4ug; Detection:20988-1-AP 1:1000) with HeLa cells lysate 2000ug.
GTX89366 (3.8µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
GTX89366 (0.1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing Human TSC1 (RC213332) and probed with GTX89366 (mock transfection in first lane)
Antigen: bs-5600P, 0.2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;TMB staining;Read the data in MicroplateReader by 450
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC1 (RC213332, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC1.
ELISA: TSC1 Antibody
Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-TSC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801949)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TSC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801949)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TSC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801949)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TSC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801949)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TSC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801949)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TSC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801949)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TSC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801949)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TSC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801949)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC1 (RC213332, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC1.
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-TSC1 monoclonal antibody at 1:200 dilution.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TSC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801951)
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-TSC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801951)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TSC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801951)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TSC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801951)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC1 (RC213332, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC1 (RC213332, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC1 (RC213332, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC1 (RC213332, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC1.
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA801949 | TSC1 / Hamartin antibody |
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Mouse | IgG2a | OTI3A2 | Purified | Hu | P, WB |
0.1 ml /
€325.00
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| OriGene Technologies, Inc. | |||||||
 +9 additional images |
Highly validated antibody!
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| TA801951 | TSC1 / Hamartin antibody |
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 |
Mouse | IgG2b | OTI5A8 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA302506 | TSC1 / Hamartin (Isoform 1) antibody |
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 |
Goat | Aff - Purified | Can, Hu, Ms, Rt | P, WB |
0.1 mg /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| TA306217 | TSC1 / Hamartin (C-term) antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu, Ms | E, WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA306218 | TSC1 / Hamartin (Center) antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA347864 | TSC1 / Hamartin antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA590017 | TSC1 / Hamartin antibody |
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 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA801952 | TSC1 / Hamartin antibody |
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 |
Mouse | IgG1 | OTI2G3 | Purified | Hu | WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
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| TA802012 | TSC1 / Hamartin antibody |
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Mouse | IgG2a | OTI2B2 | Purified | Hu | WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA802093 | TSC1 / Hamartin antibody |
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 |
Mouse | IgG2a | OTI5A7 | Purified | Hu | WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AP07383PU-N | TSC1 / Hamartin antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms | P, WB |
50 µg /
€470.00
|
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| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| AP07800PU-N | TSC1 / Hamartin antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
50 µg /
€470.00
|
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| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| AP12886PU-N | TSC1 / Hamartin pSer505 antibody |
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Rabbit | Ig | Aff - Purified | Hu |
0.4 ml /
€370.00
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| Acris Antibodies GmbH | |||||||
| AP30959CP-N | TSC1 / Hamartin Control Peptide |
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 |
50 µg /
€160.00
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| Acris Antibodies GmbH | |||||||
| AP30960CP-N | TSC1 / Hamartin Control Peptide |
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50 µg /
€160.00
|
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| Acris Antibodies GmbH | |||||||
| SP2163P | TSC1 / Hamartin antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 mg /
€370.00
|
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| Acris Antibodies GmbH | |||||||
| SP2163S | TSC1 / Hamartin antibody |
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Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | ICC/IF, WB |
50 µg /
€300.00
|
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| Acris Antibodies GmbH | |||||||
| H00007248-A01 | TSC1 / Hamartin antibody |
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Mouse | Serum | Hu | E |
50 µl /
€290.00
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| Abnova Taiwan Corp. | |||||||
| H00007248-B01P | TSC1 / Hamartin antibody |
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Mouse | Hu | WB |
0.5 mg /
€1,400.00
|
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| Abnova Taiwan Corp. | |||||||
| PAB7016 | TSC1 / Hamartin antibody |
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|
Goat | Hu | E, WB |
0.1 mg /
€400.00
|
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| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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