TSC22D3 antibody
Principal name
TSC22D3 antibody
Alternative names for TSC22D3 antibody
TSC22 domain family protein 3, TSC-22-like protein, TSC-22R, DSIP-immunoreactive peptide, DSIPI, GILZ, Delta sleep-inducing peptide immunoreactor, Glucocorticoid-induced leucine zipper protein
Ncbi ID
9606, NP_001015881, NP_001015881.1, NP_004080.2, NM_004089, NP_004080, NP_001070832.1, NM_001077364, NM_031345, NM_198057, NM_001015881
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Flow Cytometry (F)
Background of TSC22D3 antibody
- Kit Component:
- - KN318324G1, Tsc22d3 gRNA vector 1 in pCas-Guide vector
- KN318324G2, Tsc22d3 gRNA vector 2 in pCas-Guide vector
- KN318324D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Asselin-Labat M.-L., et al. (2004) Blood. 104:215-223Berrebi D., et al. (2003) Blood. 101:729-738
Immunoperoxidase of monoclonal antibody to TSC22D3 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml]
Immunofluorescence of monoclonal antibody to TSC22D3 on HeLa cell . [antibody concentration 10 ug/ml]
Detection limit for recombinant GST tagged TSC22D3 is approximately 0.03ng/ml as a capture antibody.
TSC22D3 polyclonal antibody (A01), Lot # 051018JC01. Western Blot analysis of TSC22D3 expression in Daoy. isoform:22.213KDa
Western Blot analysis of TSC22D3 expression in transfected 293T cell line (H00001831-T01) by TSC22D3 MaxPab polyclonal antibody.Lane 1: TSC22D3 transfected lysate(22 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of TSC22D3 expression in transfected 293T cell line (H00001831-T01) by TSC22D3 MaxPab polyclonal antibody.Lane 1: TSC22D3 transfected lysate(22.20 KDa).Lane 2: Non-transfected lysate.
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to TSC22D3 on HeLa cell (antibody concentration 10 µg/ml).
Western Blot analysis of TSC22D3 expression in transfected 293T cell line. Lane 1: Transfected Cell Lysate Lane 2: Non-transfected cell lysate
Detection limit for recombinant GST tagged TSC22D3 is approximately 0.03 ng/ml as a capture antibody.
TSC22D3 Antibody (Center) (AP54370PU-N) western blot analysis in mouse kidney tissue lysates (35 µg/lane). This demonstrates the TSC22D3 antibody detected the TSC22D3 protein (arrow).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC22D3 (RC210088, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC22D3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC22D3 (RC210088, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC22D3.
Anti-TSC22D3 mouse monoclonal antibody (TA505357) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TSC22D3(RC210088).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC22D3 (RC210088, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC22D3.
Anti-TSC22D3 mouse monoclonal antibody (TA505368) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TSC22D3(RC210088).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC22D3 (RC210088, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC22D3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC22D3 (RC210088, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC22D3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC22D3 (RC210088, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC22D3.
Western blot analysis of TSC22D3 in human small intestine tissue lysate with TSC22D3 antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry of TSC22D3 in human small intestine tissue with TSC22D3 antibody at 5 ug/mL.
Immunofluorescence of TSC22D3 in human small intestine tissue with TSC22D3 antibody at 20 ug/mL.
Left: Immunoblot of mouse thymocytes cultured without (lane 1) or with (lane 2) dexamethasone (10e-7 M) for 3 hr. The membrane was probed with 10 ug/mL of Anti-Human/Mouse GILZ Purified, followed by Anti-Rat IgG HRP. Right: Immunoblot of normal human peripheral blood cells cultured without (lane 1) or with (lane 2) dexamethasone (10e-7 M) for 3 hr. The membrane was probed with 2 ug/mL of Anti-Human/Mouse GILZ Purified, followed by Anti-Rat IgG HRP.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM20880PU-N | TSC22D3 antibody |
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Mouse | IgG1 | 3A5 | Hu | E, ICC/IF, P, WB |
50 µg /
€475.00
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| OriGene Technologies GmbH | |||||||
 +4 additional images |
Highly validated antibody! |
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| TA319636 | TSC22D3 antibody |
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| Rabbit Polyclonal TSC22D3 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
€415.00
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA320507 | TSC22D3 antibody |
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| Rat Anti-Human/Mouse GILZ Purified (25 ug) | |||||||
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Rat | CFMKG15 | Purified | Ms | F, ICC/IF, WB |
25 µg /
€165.00
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|
| OriGene Technologies, Inc. | |||||||
| TA350019 | TSC22D3 antibody |
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| Rabbit Polyclonal Anti-TSC22D3 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
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|
| OriGene Technologies, Inc. | |||||||
| TA505293 | TSC22D3 antibody |
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| TSC22D3 mouse monoclonal antibody, clone OTI1F11 (formerly 1F11) | |||||||
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Mouse | IgG1 | OTI1F11 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| TA505355 | TSC22D3 antibody |
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| TSC22D3 mouse monoclonal antibody, clone OTI1H3 (formerly 1H3) | |||||||
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Mouse | IgG2b | OTI1H3 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| TA505357 | TSC22D3 antibody |
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| TSC22D3 mouse monoclonal antibody, clone OTI3F3 (formerly 3F3) | |||||||
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Mouse | IgG2b | OTI3F3 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA505368 | TSC22D3 antibody |
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| TSC22D3 mouse monoclonal antibody, clone OTI1E11 (formerly 1E11) | |||||||
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Mouse | IgG1 | OTI1E11 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA505378 | TSC22D3 antibody |
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| TSC22D3 mouse monoclonal antibody, clone OTI5D3 (formerly 5D3) | |||||||
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Mouse | IgG1 | OTI5D3 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| TA505380 | TSC22D3 antibody |
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| TSC22D3 mouse monoclonal antibody, clone OTI2D2 (formerly 2D2) | |||||||
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Mouse | IgG1 | OTI2D2 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| AP54370PU-N | TSC22D3 (Center) antibody |
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Rabbit | Ig | Aff - Purified | Hu, Ms | WB |
0.4 ml /
€415.00
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|
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN318324BN | Tsc22d3 - mouse gene knockout kit via CRISPR |
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Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN318324LP | Tsc22d3 - mouse gene knockout kit via CRISPR |
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Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN318324RB | Tsc22d3 - mouse gene knockout kit via CRISPR |
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Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP310088 | TSC22D3 (transcript variant 2) |
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| Recombinant protein of human TSC22 domain family, member 3 (TSC22D3), transcript variant 2 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| AR50584PU-N | TSC22D3 (1-134, His-tag) |
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Human | Purified | > 85 % by SDS - PAGE | E. coli |
0.25 mg /
€1,070.00
|
| OriGene Technologies GmbH | |||||
| AR50584PU-S | TSC22D3 (1-134, His-tag) |
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Human | Purified | > 85 % by SDS - PAGE | E. coli |
50 µg /
€400.00
|
| OriGene Technologies GmbH | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY418232 | TSC22D3 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC214184L2V | Lenti ORF particles, TSC22D3 (mGFP-tagged)-Human TSC22 domain family, member 3 (TSC22D3), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RC210088L2V | Lenti ORF particles, TSC22D3 (mGFP-tagged) - Human TSC22 domain family, member 3 (TSC22D3), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
