TTF1 / NKX2A antibody

Principal name

TTF1 / NKX2A antibody

Alternative names for TTF1 / NKX2A antibody

NKX2-1, Nkx-2.1, TITF1, Thyroid nuclear factor 1, Thyroid transcription factor 1, TTF-1, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A

SwissProt ID

P23441 (Rat), P43699 (Human), P50220 (Mouse)

Gene ID

7080 (NKX2-1), 7270 (TTF1), 5395 (PMS2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), Sh (Sheep), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Frozen Sections (C), Immunoprecipitation (IP)

Background of TTF1 / NKX2A antibody

The thyroid transcription factor 1 (TTF-1) is a homeodomain-containing transcription factor that activates the transcriptional activity of thyroid-specific gene promoters by binding to them. Hence, TTF-1 is crucial in the maintenance of the thyroid differentiation phenotype (1). Thyroid dysgenesis (TD) is responsible for most cases of congenital hypothyroidism, a condition that affects about one in 4000 newborns. Mutations in PAX8, TTF1, or FOXE1 may account for congenital hypothyroidism in patients with either isolated TD or TD with associated malformations involving kidney, lung, forebrain, and palate (2). In addition to its presence in thyroid gland epithelium, the human TTF-1 protein was detected by immunohistochemistry in human fetal lung as early as 11 weeks of gestation, being localized in the nuclei of epithelial cells of the developing airways. After birth, TTF-1 was selectively expressed in Type II epithelial cells in the alveoli and in subsets of bronchiolar epithelial cells in the conducting regions of the lung (3).

General readings

1. Lin, S., et al., J. Biol. Chem. 281, 16716-16726 (2006)
2. Palma, T. D., et al., J. Biol. Chem. 278, 3395-3402 (2003)
3. Weidenfeld, J., J. Biol. Chem. 277, 21061-21070 (2002) 4) Kramer, B. W., et al., Am. J. Physiol. Lung Cell Mol. Physiol. 283, 452-459 (2002)
5. Ramos-Vara, J. A., et al., Vet. Pathol. 39, 480-487 (2002)
6. Kohtz, J. D., et al., Development 125, 5079-5089 (1998)
7. Holzinger, A., et al., Hybridoma 15, 49-53 (1996)
Clone 8G7G3/1 is used in these references.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TTF1 / NKX2A antibody

Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-NKX2-1 Mouse monoclonal antibody. (UM800057, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG1 UMAB162 Purified Hu P
0.1 ml / €379.00
  OriGene Technologies, Inc.

UltraMAB

TTF1 / NKX2A antibody

Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-NKX2-1 Mouse monoclonal antibody. (UM800057, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG1 UMAB162 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

TTF1 / NKX2A antibody

Formalin-Fixed, Paraffin-Embedded Human Lung Adenocarcinoma stained with TTF1 / NKX2A antibody (Cat.-No AM11200PU). Mouse IgG1 8G7G3/1 Purified Hu, Ms, Rt P
0.5 ml / €410.00
  Acris Antibodies GmbH

TTF1 / NKX2A antibody

Formalin-Fixed, Paraffin-Embedded Human Lung Adenocarcinoma stained with TTF1 / NKX2A antibody (Cat.-No AM11200PU). Mouse IgG1 8G7G3/1 Purified Hu, Ms, Rt P
1 ml / €580.00
  Acris Antibodies GmbH

TTF1 / NKX2A antibody

Formalin-Fixed, Paraffin-Embedded Human Lung Adenocarcinoma stained with TTF1 / NKX2A antibody (Cat.-No AM11200PU). Mouse IgG1 8G7G3/1 Purified Hu, Ms, Rt P
0.1 ml / €220.00
  Acris Antibodies GmbH

TTF1 / NKX2A (N-term) antibody

Anti-TTF-1 (DB 088)Western blot of TTF-1: lane 1 – 5 μl; lane 2 – 15 μl of transient overexpression cell lysate (1 μg/μl of total crude protein) of recombinant human TTF-1 (H00007080-T01). Rabbit IgG A22-A Aff - Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
0.1 ml / €550.00
  DB Biotech, spol. s r.o.

Sample sizes available

TTF1 / NKX2A (N-term) antibody

Anti-TTF-1 (DB 088)Western blot of TTF-1: lane 1 – 5 μl; lane 2 – 15 μl of transient overexpression cell lysate (1 μg/μl of total crude protein) of recombinant human TTF-1 (H00007080-T01). Rabbit IgG A22-A Aff - Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
50 µl / €370.00
  DB Biotech, spol. s r.o.

Sample sizes available

TTF1 / NKX2A (N-term) antibody

Formalin-Fixed, Paraffin-Embedded Human thyroid gland tissue (4 μm) stained with anti-TTF-1 antibody (DB 089) shows strong positive nuclear immunostaining of follicular cells. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph.D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG G21-G Aff - Purified C, P
0.1 ml / €320.00
  DB Biotech, spol. s r.o.

Sample sizes available

TTF1 / NKX2A (N-term) antibody

Formalin-Fixed, Paraffin-Embedded Human thyroid gland tissue (4 μm) stained with anti-TTF-1 antibody (DB 089) shows strong positive nuclear immunostaining of follicular cells. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph.D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG G21-G Aff - Purified Hu C, P
0.2 ml / €440.00
  DB Biotech, spol. s r.o.

Sample sizes available

TTF1 / NKX2A (N-term) antibody

Formalin-Fixed, Paraffin-Embedded Human thyroid gland tissue (4 μm) stained with anti-TTF-1 antibody (DB 089) shows strong positive nuclear immunostaining of follicular cells. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph.D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG G21-G Aff - Purified C, P
0.5 ml / €550.00
  DB Biotech, spol. s r.o.

Sample sizes available

TTF1 / NKX2A (N-term) antibody

Formalin-Fixed, Paraffin-Embedded Human thyroid gland tissue (4 μm) stained with anti-TTF-1 antibody (DB 089) shows strong positive nuclear immunostaining of follicular cells. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph.D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG G21-G Aff - Purified Hu C, P
1 ml / €870.00
  DB Biotech, spol. s r.o.

Sample sizes available

TTF1 / NKX2A antibody

Formalin-Paraffin lung adenocarcinoma (20X) stained with TTF-1 Antibody Cat.-No AM50175PU (Clone SPM150). Mouse IgG1 SPM150 Purified Hu, Ms, Rt C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

TTF1 / NKX2A antibody

Formalin-Paraffin lung adenocarcinoma (20X) stained with TTF-1 Antibody Cat.-No AM50175PU (Clone SPM150). Mouse IgG1 SPM150 Purified Hu, Ms, Rt C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

TTF1 / NKX2A antibody

Formalin-Paraffin lung adenocarcinoma (20X) stained with TTF-1 Antibody Cat.-No AM50175PU (Clone SPM150). Mouse IgG1 SPM150 Purified Hu, Ms, Rt C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

TTF1 / NKX2A (N-term) antibody

Western blot analysis of NKX2-1 in rat lung tissue lysate with NKX2-1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TTF1 / NKX2A antibody

Predicted band size: 42 kDa . Positive control: Mouse lung tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TTF1 / NKX2A antibody

Predicted band size: 42 kDa . Positive control: Mouse lung tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TTF1 / NKX2A antibody

Immunohistochemistry staining of Paraffin Lung adenocarcinoma tissue by TTF-1 antibody (dilution: 1:100 - 1:500; visualization of staining: Nuclear) Mouse IgG1 8G7G3/1 Purified Hu P
1 ml / €340.00
  OriGene Technologies, Inc.

TTF1 / NKX2A antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NKX2 (RC217520, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NKX2. Mouse IgG1 OTI2H10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TTF1 / NKX2A antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NKX2-1 (RC217520, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NKX2-1. Mouse IgG1 OTI3F3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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