TUBB / TUBB5 antibody

Principal name

TUBB / TUBB5 antibody

Alternative names for TUBB / TUBB5 antibody

Tubulin beta chain, Tubulin beta-5 chain

SwissProt ID

P07437 (Human), P09653 (Chick), P69897 (Rat), P99024 (Mouse), Q2KJD0 (Bovin), Q6NW90, Q767L7 (Pig), Q91575 (Xenla)

Gene ID

203068 (TUBB), 386701 (tubb5)

Available reactivities

Chk (Chicken), Mam (Mammalia), Xen (Xenopus), All Species, Bov (Bovine), Fu (Fungi), GP (Guinea Pig), Ge (Gerbil), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Sea Urchin, Can (Canine), Hst (Hamster), Rb (Rabbit), Chimp (Chimpanzee), Carp, Pl (Plant), Mky (Monkey), Ze (Zebrafish), Frog, Ye (Yeast), African clawed frog, Dictyostelium, Insect, Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E), Frozen Sections (C)

Background of TUBB / TUBB5 antibody

The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening - this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains.
The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.

General readings

General / background references:Audebert S et al. The carboxy-terminal sequence Asp427-Glu432 of beta-tubulin plays an important function in axonemal motility. Eur J Biochem 261:48-56 (1999). PubMed PMID: 10103032
Gingras D et al. Molecular cloning and characterization of a radial spoke head protein of sea urchin sperm axonemes: involvement of the protein in the regulation of sperm motility. Mol Biol Cell 9:513-22 (1998). PubMed PMID: 9450971
Roach MC et al. Preparation of a monoclonal antibody specific for the class I isotype of beta-tubulin: the beta isotypes of tubulin differ in their cellular distributions within human tissues. Cell Motil Cytoskeleton 39:273-85 (1998). PubMed PMID: 9580378
Banerjee A et al. Increased microtubule assembly in bovine brain tubulin lacking the type III isotype of beta-tubulin. J Biol Chem 265:1794-9 (1990). PubMed PMID: 2404018

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TUBB / TUBB5 antibody

Sample (30 ug of whole cell lysate). A: NIH-3T3. 10% SDS PAGE. TA309059 diluted at 1:5000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TUBB / TUBB5 antibody

Western blot detection of Tubulin beta in COS7,LNCap,MCF7 and C2C12 cell lysates and using Tubulin beta mouse mAb (1:5000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa. Mouse IgG1 1E1-E8-H4 Purified Hst ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TUBB / TUBB5 (C-term) antibody

Human lung stained with
Anti-Tubulin beta antibody. Rabbit Purified Bov, Chk, Frog, Fu, Ge, GP, Hu, Ms, Por, Rt, Sea Urchin, Ye IP, P, WB
1 ml / €440.00
  Acris Antibodies GmbH

TUBB / TUBB5 (C-term) antibody

Human lung stained with
Anti-Tubulin beta antibody. Rabbit Purified Bov, Chk, Frog, Fu, Ge, GP, Hu, Ms, Por, Rt, Sea Urchin, Ye IP, P, WB
0.1 ml / €140.00
  Acris Antibodies GmbH

TUBB / TUBB5 (C-term) antibody

Human lung stained with
Anti-Tubulin beta antibody. Rabbit Purified Bov, Chk, Frog, Fu, Ge, GP, Hu, Ms, Por, Rt, Sea Urchin, Ye IP, P, WB

Regular Price: 25 µl / €80.00

Special Price: 25 µl / €56.00

  Acris Antibodies GmbH

TUBB / TUBB5 antibody

Detection of Beta-Tubulin in HeLa whole cell lysate at a 1:1,000 dilution. Rabbit IgG Purified Chk, Hu, Ms, Por, Rt, Xen, Ze WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TUBB / TUBB5 antibody

Anti-TUBB / Tubulin, Beta antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Mouse IgG3 n.a Purified Bov, Chk, Frog, Hu, Ms, Rb, Rt E, ICC/IF, P, WB
50 µl / €345.00
  OriGene Technologies, Inc.

TUBB / TUBB5 antibody

Western blot analysis of anti-TBB5 Antibody (Cat. #TA324326) in Y79,CEM,293 cell line and mouse brain tissue lysates (35ug/lane). TBB5(arrow) was detected using the purified Mab.(1ug/ml) Mouse IgG2b 87CT59.3.7 Purified Bov, Chk, Hst, Hu, Ms, Por, Rt, Xen ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

TUBB / TUBB5 antibody

Western Blot: beta II Tubulin A Antibody [TA336361] - analysis of b-Tubulin in A: human brain, B: mouse brain and C: rat brain tissue lysates using the this antibody. 25 ug/ml. Rabbit Purified Bov, Can, Chk, Hu, Ms, Rt C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TUBB / TUBB5 antibody

Western blot detection of Tubulin beta in Jurkat,K562,C6,3T3 and Hela cell lysates and using Tubulin beta mouse mAb (1:1000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa. Mouse IgG1 1E1-E8-H4 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TUBB / TUBB5 antibody

TUBB / TUBB5 Mouse IgM TU-12 Purified All Species ICC/IF, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

TUBB / TUBB5 antibody

TUBB / TUBB5 Mouse IgM TU-13 Purified Hu, Ms, Pl, Por C, E, ICC/IF, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

TUBB / TUBB5 antibody

Western blot analysis of HeLa cell lysate, using beta-Tubulin (10B1) Mouse mAb with 1/2000, 1/5000(lane 2) and 1/10000 dilutions. Mouse IgG1 TUB-2 Purified Hu, Rt IP, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

TUBB / TUBB5 (Loading Control) antibody

Western Blot Analysis of 293 cell lysates with Mouse Monoclonal anti-beta-Tubulin antibody (Lane 1-8). Mouse IgG1 A10 Aff - Purified Dictyostelium, Hu, Ms, Saccharomyces, Tetrahymena ICC/IF, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

TUBB / TUBB5 (Loading Control) antibody

Western Blot Analysis of 293 cell lysates with Mouse Monoclonal anti-beta-Tubulin antibody (Lane 1-8). Mouse IgG1 A10 Aff - Purified Dictyostelium, Hu, Ms, Saccharomyces, Tetrahymena ICC/IF, WB
50 µg / €230.00
  Acris Antibodies GmbH

TUBB / TUBB5 (N-term) antibody

AP08520PU-N TUBB antibody staining of Formalin-Fixed Paraffin-Embedded Human Thymus at 1/100 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Purified Hu, Mky, Ms, Rt ICC/IF, P
50 µl / €470.00
  Acris Antibodies GmbH

TUBB / TUBB5 (N-term) (Loading Control) antibody

Western Blotting analysis (reducing conditions) of HPB-ALL human peripheral blood leukemia cell line. Lane 1: negative control. Lane 2,3,4,5,6: immunostaining with anti-beta-tubulin (TU-06; dilution 0,5 μg/ml, 1 μg/ml, 2 μg/ml, 4 μg/ml, 5 μg/ml) Mouse IgM TU-06 Purified All Species ICC/IF, P, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

TUBB / TUBB5 antibody

Immunoperoxidase of monoclonal antibody to TUBB on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml] Mouse IgG2a 3B3 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TUBB / TUBB5 antibody

Western blot analysis of TUBB/TUBB2A/TUBB1 expression in HeLa cell lysate. Rabbit Hu, Ms, Rt WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

TUBB / TUBB5 antibody

Immunohistochemical staining of TUBB on formalin fixed, paraffin embedded human lung with TUBB polyclonal antibody. Rabbit Hu IP, P, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

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