TUBB / TUBB5 antibody

Principal name

TUBB / TUBB5 antibody

Alternative names for TUBB / TUBB5 antibody

Tubulin beta chain, Tubulin beta-5 chain

SwissProt ID

P07437 (Human), P09653 (Chick), P69897 (Rat), P99024 (Mouse), Q2KJD0 (Bovin), Q6NW90, Q767L7 (Pig), Q91575 (Xenla)

Gene ID

203068 (TUBB), 22154 (Tubb5), 29214

Available reactivities

All Species, Hu (Human), Ms (Mouse), Pl (Plant), Por (Porcine), Bov (Bovine), Chk (Chicken), Frog, Fu (Fungi), GP (Guinea Pig), Ge (Gerbil), Rt (Rat), Sea Urchin, Ye (Yeast), Xen (Xenopus), Mky (Monkey), Can (Canine), Ze (Zebrafish), African clawed frog, Dictyostelium, Rb (Rabbit), Hst (Hamster), Insect, Sh (Sheep)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of TUBB / TUBB5 antibody

The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening - this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains.
The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TUBB / TUBB5 antibody

  Rabbit Polyclonal antibody to beta Tubulin (tubulin, beta)  
Sample (30 ug of whole cell lysate). A: NIH-3T3. 10% SDS PAGE. TA309059 diluted at 1:5000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €416.00
  OriGene Technologies, Inc.

TUBB / TUBB5 antibody

  Mouse Monoclonal beta Tubulin Antibody  
Western blot detection of Tubulin beta in COS7,LNCap,MCF7 and C2C12 cell lysates and using Tubulin beta mouse mAb (1:5000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa. Mouse IgG1 1E1-E8-H4 Purified Hst ICC/IF, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

TUBB / TUBB5 antibody

  Rabbit polyclonal anti-TUBB(beta Tubulin) antibody, Loading control  
Detection of Beta-Tubulin in HeLa whole cell lysate at a 1:1,000 dilution. Rabbit IgG Purified Chk, Hu, Ms, Por, Rt, Xen, Ze WB
0.1 ml / €451.00
  OriGene Technologies, Inc.

TUBB / TUBB5 antibody

  TUBB / Beta Tubulin Mouse Monoclonal Antibody  
TUBB / TUBB5 Mouse IgG3 n.a Purified Bov, Chk, Frog, Hu, Ms, Rb, Rt E, ICC/IF, P, WB
50 µl / €400.00
  OriGene Technologies, Inc.

TUBB / TUBB5 antibody

  Mouse monoclonal TBB5 Antibody  
Western blot analysis of anti-TBB5 Antibody (Cat. #TA324326) in Y79,CEM,293 cell line and mouse brain tissue lysates (35ug/lane). TBB5(arrow) was detected using the purified Mab.(1ug/ml) Mouse IgG2b 87CT59.3.7 Purified Bov, Chk, Hst, Hu, Ms, Por, Rt, Xen ICC/IF, P, WB
0.4 ml / €410.00
  OriGene Technologies, Inc.

TUBB / TUBB5 antibody

  Rabbit Polyclonal beta Tubulin Antibody  
Western Blot: beta II Tubulin A Antibody [TA336361] - analysis of b-Tubulin in A: human brain, B: mouse brain and C: rat brain tissue lysates using the this antibody. 25 ug/ml. Rabbit Purified Bov, Can, Chk, Hu, Ms, Rt C, P, WB
0.1 mg / €393.00
  OriGene Technologies, Inc.

TUBB / TUBB5 antibody

  Mouse Monoclonal beta Tubulin Antibody  
Western blot detection of Tubulin beta in Jurkat,K562,C6,3T3 and Hela cell lysates and using Tubulin beta mouse mAb (1:1000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa. Mouse IgG1 1E1-E8-H4 Purified WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

TUBB / TUBB5 antibody

TUBB / TUBB5 Mouse IgM TU-12 Purified All Species ICC/IF, WB
0.1 mg / €240.00
  OriGene Technologies GmbH

TUBB / TUBB5 antibody

TUBB / TUBB5 Mouse IgM TU-13 Purified Hu, Ms, Pl, Por C, E, ICC/IF, WB
0.1 mg / €240.00
  OriGene Technologies GmbH

TUBB / TUBB5 antibody

Western blot analysis of HeLa cell lysate, using beta-Tubulin (10B1) Mouse mAb with 1/2000, 1/5000(lane 2) and 1/10000 dilutions. Mouse IgG1 TUB-2 Purified Hu, Rt IP, P, WB
0.1 mg / €390.00
  OriGene Technologies GmbH

TUBB / TUBB5 (N-term) antibody

AP08520PU-N TUBB antibody staining of Formalin-Fixed Paraffin-Embedded Human Thymus at 1/100 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Purified Hu, Mky, Ms, Rt ICC/IF, P
50 µl / €380.00
  OriGene Technologies GmbH

TUBB / TUBB5 (N-term) (Loading Control) antibody

Western Blotting analysis (reducing conditions) of HPB-ALL human peripheral blood leukemia cell line. Lane 1: negative control. Lane 2,3,4,5,6: immunostaining with anti-beta-tubulin (TU-06; dilution 0,5 μg/ml, 1 μg/ml, 2 μg/ml, 4 μg/ml, 5 μg/ml) Mouse IgM TU-06 Purified All Species ICC/IF, P, WB
0.1 mg / €240.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TUBB / TUBB5

  Recombinant protein of human tubulin, beta (TUBB)  
TUBB / TUBB5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

13 Item(s)

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