TUBB3 / TUBB4 antibody

Principal name

TUBB3 / TUBB4 antibody

Alternative names for TUBB3 / TUBB4 antibody

Tubulin beta-3 chain, Tubulin beta-III, Tubulin beta-4

SwissProt ID

P08841 (Drome), P09206 (Chick), P28551 (Soybn), Q13509 (Human), Q2T9S0 (Bovin), Q4QRB4 (Rat), Q9ERD7 (Mouse)

Gene ID

10381 (TUBB3), 246118 (Tubb3)

Available reactivities

Broad, Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Fi (Fish), Mam (Mammalia), Por (Porcine), Bab (Baboon), Hst (Hamster), Carp, All Species, Can (Canine), Insect, Mky (Monkey), Rb (Rabbit), Sh (Sheep), Ye (Yeast)

Available hosts

Mouse, Chicken, Rabbit, Goat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of TUBB3 / TUBB4 antibody

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy-terminal. For beta-tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta- tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules. The monoclonal antibody from hybridoma SDL.3D10 can stimulate microtubule assembly when reconstituted with tubulin, tau or MAP2.

General readings

General / background references:Banerjee A et al. Increased microtubule assembly in bovine brain tubulin lacking the type III isotype of beta-tubulin. J Biol Chem 265:1794-9 (1990). PubMed PMID: 2404018
Joshi HC & Cleveland DW Diversity among tubulin subunits: toward what functional end? Cell Motil Cytoskeleton 16:159-63 (1990). PubMed PMID: 2194680
Banerjee A et al. A monoclonal antibody against the type II isotype of beta-tubulin. Preparation of isotypically altered tubulin. J Biol Chem 263:3029-34 (1988). PubMed PMID: 3277964

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TUBB3 / TUBB4 antibody

Tuj-1 staining of Neuron-specific class III ?-tubulin in differentiated human neural progenitor cells. Cells were stained using goat anti-mouse Alexa Fluor 488 (green) secondary antibody (Molecular Probe, A-11001) and counterstained with PI (red). Mouse IgG2a TuJ-1 Purified Hu, Rt ICC/IF, WB
0.1 mg / €350.00
  Neuromics Antibodies

TUBB3 / TUBB4 antibody

TUBB3 / TUBB4 Mouse IgG1 OTI5H2 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

TUBB3 / TUBB4 antibody

AP32033PU-N TUBB3 Antibody staining of HepG2 lysate at 2 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

TUBB3 / TUBB4 antibody

TUBB3 / TUBB4 Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TUBB3 / TUBB4 antibody

Western blot of rat cortex lysate showing specific immunolabeling of the ~55k beta III tubulin protein. Mouse IgG AA10 Purified Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TUBB3 / TUBB4 antibody

Western blot analysis of extracts from rat cells and mouse brain cells, using Tubulin ß antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TUBB3 / TUBB4 antibody

Predicted band size: 50kDa. Positive control: Human brain glioma tissue lysate. Recommended dilution: 1/500-3000. (Gel: 8%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TUBB3 / TUBB4 antibody

Western blot analysis of Tubulin beta expression in HeLa,Rat brain,Mouse brain whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TUBB3 / TUBB4 antibody

Western Blot: Tubulin Beta 3 Antibody [TA336546] - at 1:500 dilution. 10 ug of normal human brain lysate loaded. Rabbit Purified Hu, Ms C, E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TUBB3 / TUBB4 antibody

Western Blot: Tubulin Beta 3 Antibody (2E9) [TA336754] - Analysis of Tubulin Beta 3 mouse mAb against HepG2 (1), A549 (2) and Hela (3) cell lysates. Mouse IgG1 2E9 Purified Hu E, F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TUBB3 / TUBB4 antibody

Immunofluorescence analysis of PANC-1 cells using TUBB3 antibody Cat.-No AM06402SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 2E9 Ascites Hu E, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

TUBB3 / TUBB4 (tubulin beta-3 chain) antibody

Breast cancer stained in Immunohistochemistry with Tubulin beta-3 antibody Cat.-No AM10229PU-N Mouse IgG1 3D9 Purified Hu C, E, IP, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TUBB3 / TUBB4 (436-450) antibody

Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a n.a Purified Bear, Bov, Can, Hu, Mky, Ms, Platypus, Por, Prim, Rt, Xen ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TUBB3 / TUBB4 antibody

AP31148PU-N Tuj1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Testis at 5 µg/ml after heat-induced antigen retrieval. Chicken IgY Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

TUBB3 / TUBB4 antibody

Mouse Brain: Formalin-Fixed, Paraffin-Embedded (FFPE) Chicken IgY Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
75 µg / €470.00
  Acris Antibodies GmbH

TUBB3 / TUBB4 antibody

Dissociated cell culture of a neonatal Mouse brain showing Beta-Tubulin III (green staining) of neurons. These cultures were counter-stained with a Rabbit anti-GFAP antibody (red staining) to identify astrocytes, and with DAPI (blue staining) to localize nuclei. Chicken Ig Aff - Purified Hu, Rt ICC/IF, P, WB
0.3 mg / €440.00
  Acris Antibodies GmbH

TUBB3 / TUBB4 antibody

BM170 TUBB3/TUBB4 antibody staining of Rat Skin Paraffin Section. Mouse IgG1 TU-20 Purified Broad C, E, F, ICC/IF, P, WB
0.2 mg / €350.00
  Acris Antibodies GmbH

TUBB3 / TUBB4 (N-term) antibody

TUBB3 / TUBB4 Mouse IgG1 TU-20 FITC All Species C, E, F, ICC/IF, P, WB
0.1 mg / €310.00
  Acris Antibodies GmbH

TUBB3 / TUBB4 antibody

Figure 2. Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid. 
Figure 2A (Left): Microtubules decorated with neuron-specific anti-betaIII-tubulin (TU-20; red). 
Figure 2B (Right): Merged image of co-staining with anti-beta-tubulin (TU-06; green; BM169S). Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue). Mouse IgG1 TU-20 Purified Broad C, E, F, ICC/IF, P, WB
0.1 mg / €250.00
  Acris Antibodies GmbH

TUBB3 / TUBB4 antibody

Western Blot analysis of TUBB3 expression in transfected 293T cell line (H00010381-T02) by TUBB3 MaxPab polyclonal antibody.

Lane 1: TUBB3 transfected lysate(50.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

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