TUBG1 / Tubulin gamma 1 antibody

Principal name

TUBG1 / Tubulin gamma 1 antibody

Alternative names for TUBG1 / Tubulin gamma 1 antibody

Tubulin gamma-1 chain, Gamma-1-tubulin, GCP-1, Gamma-tubulin complex component 1

SwissProt ID

P23257 (Drome), P23258 (Human), P23330 (Xenla), P83887 (Mouse), P83888 (Rat), Q0VCD2 (Bovin), Q7ZVM5, Q9GKK5 (Canfa)

Gene ID

7283 (TUBG1), 4393882

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Xen (Xenopus), Sea Urchin, Pl (Plant), Por (Porcine), Pr (Protozoa), Mky (Monkey), Mam (Mammalia), All Species, Amph (Amphibian), Av (Birds), Bac (Bacteria), Ce (C. elegans), Do (Dolphin), Dros (Drosophila), Eq (Equine), Fe (Feline), Ft (Ferret), Fi (Fish), Fu (Fungi), GP (Guinea Pig), Gt (Goat), Op (Opossum), Pp (Photinus pyralis), Rb (Rabbit), Re (Reptiles), Sh (Sheep), Ye (Yeast), Ze (Zebrafish), Ll (Llama), Vi (Virus), Bab (Baboon)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of TUBG1 / Tubulin gamma 1 antibody

The protein that binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome is called gamma tubulin. Gamma Tubulin functions as the microtubule nucleator at the microtubule organizing centre (MTOC). By binding to the beta tubulin half of the tubulin molecule, it establishes the polarity of a microtubule, leaving the alpha tubulin half exposed at the positive end. Gamma Tubulin (approx. 48 kD) is a ubiquitous and highly conserved protein within the MTOCs in eukaryotic kingdom. It is related to alpha and beta tubulin and is, thus, a member of the tubulin superfamily of proteins. However, its abundance is less than 1% of the level of either alpha or beta tubulin. Moreover, unlike alpha and beta tubulin, it is not a component of microtubules. Rather, it is located at the MTOC. gamma Tubulin shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. Antibodies reacting specifically with gamma tubulin serve as an essential tool in the detection of the presence and role of this molecule in various cellular settings.

General readings

General / background references:Chang P & Stearns T Delta-tubulin and epsilon-tubulin: two new human centrosomal tubulins reveal new aspects of centrosome structure and function. Nat Cell Biol 2:30-5 (2000). PubMed PMID: 10620804
Southwick PL et al. Cyanine dye labeling reagents--carboxymethylindocyanine succinimidyl esters. Cytometry 11:418-30 (1990). PubMed PMID: 2340776
Oakley BR et al. Gamma-tubulin is a component of the spindle pole body that is essential for microtubule function in Aspergillus nidulans. Cell 61:1289-301 (1990). PubMed PMID: 2194669
Oakley CE & Oakley BR Identification of gamma-tubulin, a new member of the tubulin superfamily encoded by mipA gene of Aspergillus nidulans. Nature 338:662-4 (1989). PubMed PMID: 2649796

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TUBG1 / Tubulin gamma 1 (C-term) antibody

Testis: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.2 ml / €470.00
  Acris Antibodies GmbH

TUBG1 / Tubulin gamma 1 (C-term) antibody

Human colon carcinoma stained with anti-Tubulin gamma antibody AP15842PU Rabbit Purified Hu P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

TUBG1 / Tubulin gamma 1 (432-451) antibody

Human colon carcinoma stained with anti-Tubulin gamma antibody AP15842PU Rabbit Purified Hu P, WB
1 ml / €520.00
  Acris Antibodies GmbH

TUBG1 / Tubulin gamma 1 (C-term) antibody

Human colon carcinoma stained with anti-Tubulin gamma antibody AP15842PU Rabbit Purified Hu P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

TUBG1 / Tubulin gamma 1 antibody

Western blot analysis of extracts from mouse brain cells, using Tubulin ? antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TUBG1 / Tubulin gamma 1 antibody

Western blot analysis of Tubulin gamma expression in Mouse braintissue lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TUBG1 / Tubulin gamma 1 antibody

Western blot analysis on mouse brain cell lysate using Tubulin gamma Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TUBG1 / Tubulin gamma 1 antibody

Cell extract  from A431 cells (Lane 1) or NIH3T3 cells (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-γ-tubulin (Clone 14C11) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG2b 14C11 Purified Hu, Ms, Rt ICC/IF, WB
25 µg / €325.00
  OriGene Technologies, Inc.

TUBG1 / Tubulin gamma 1 antibody

Whole cell extract from A431 cells (Lane 1) and NIH3T3 cells  (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-?-tubulin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6209 Purified Hu, Ms ICC/IF, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

TUBG1 / Tubulin gamma 1 antibody

Figure 3. Immunofluorescence staining of microtubular networks in 3T3 Mouse fibroblasts.
3A: Metaphase, 3B: Anaphase, 3C: Telophase.
Gamma-Tubulin (red) stained with anti-gamma-Tubulin antibody (TU-30), 
Alpha-Tubulin (green) with polyclonal anti-alpha-Tubulin antibody and nuclei with DAPI (blue). Mouse IgG1 TU-30 Aff - Purified Chk, Hu, Ms, Pl, Por, Pr, Rt ICC/IF, WB
0.1 mg / €250.00
  Acris Antibodies GmbH

TUBG1 / Tubulin gamma 1 (434-449) antibody

Western blot analysis of gamma-Tubulin in porcine brain lysate by antibody TU-32. Mouse IgG1 TU-32 Aff - Purified Mam, Pl, Pr E, ICC/IF, WB
0.1 mg / €250.00
  Acris Antibodies GmbH

TUBG1 / Tubulin gamma 1 antibody

TUBG1 / Tubulin gamma 1 Mouse IgG1 IMD-20 Purified Hu, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

TUBG1 / Tubulin gamma 1 antibody

Immunohistochemistry analysis of Tubulin gamma antibody (Cat.-No AP06470PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TUBG1 / Tubulin gamma 1 antibody

Western Blot analysis of TUBG1 expression in transfected 293T cell line (H00007283-T02) by TUBG1 MaxPab polyclonal antibody.

Lane 1: TUBG1 transfected lysate(51.20 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

TUBG1 / Tubulin gamma 1 antibody

Immunofluorescence staining of microtubular networks in NIH/3T3 mouse fibroblasts. A : Metaphase. B : Anaphase. C : Telophase. Gamma - tubulin ( red ) stained with TUBG1 monoclonal antibody, clone TU - 30 ( Cat # MAB0975 ) , alpha - tubulin ( green ) with polyclonal anti - alpha - tubulin antibody and nuclei with DAPI ( blue ) . Mouse IgG1 TU-30 Chk, Hu, Ms, Rt ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

TUBG1 / Tubulin gamma 1 antibody

TUBG1 / Tubulin gamma 1 Mouse IgG1 TU-32 Hu ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

TUBG1 / Tubulin gamma 1 antibody

TUBG1 / Tubulin gamma 1 Mouse IgG1 TU-30 Chk, Hu, Ms, Pr, Rt ICC/IF, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

TUBG1 / Tubulin gamma 1 antibody

Immunohistochemical staining of TUBG1 on formalin fixed, paraffin embedded human colon carcinoma with TUBG1 polyclonal antibody. Rabbit Hu P, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

TUBG1 / Tubulin gamma 1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Tubulin gamma Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

TUBG1 / Tubulin gamma 1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Tubulin gamma Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

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