Tumor necrosis factor (TNF-alpha) antibody

Principal name

Tumor necrosis factor (TNF-alpha) antibody

Alternative names for Tumor necrosis factor (TNF-alpha) antibody

TNF, TNF-a, TNFA, TNFSF2, Cachectin, Tumor necrosis factor ligand superfamily member 2

SwissProt ID

P01375 (Human), P04924 (Rabit), P06804 (Mouse), P16599 (Rat), P19101 (Felca), P23383 (Sheep), P23563 (Pig), P29553 (Horse), P48094 (Macmu), P51435 (Cavpo), P51742 (Canfa), Q06599 (Bovin)

Gene ID

7124 (TNF), 103694380 (LOC103694380), 280943, 403922, 100033834, 397086, 21926 (Tnf), 24835

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Mky (Monkey), Por (Porcine), Bov (Bovine), Eq (Equine), Bab (Baboon), Hst (Hamster), GP (Guinea Pig), Can (Canine), Rhesus Mky (Rhesus Monkey), Prim (Primate), Fe (Feline), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Goat, Rabbit, Rat, Hamster, Chicken, Sheep

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Functional assay (FN), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP), Radioimmunoassay (R), Neutralisation (NEUT), Luminex (LMNX)

Background of Tumor necrosis factor (TNF-alpha) antibody

Tumor necrosis factor alpha (TNF-a, TNF alpha ) and the structurally related lymphotoxin-alpha are pro-inflammatory cytokines produced by a variety of immune and non-immune cells in response to multiple stimuli. These stimuli can include bacteria and bacterial products such as LPS, viral infections, T-cell receptor activation and cross-linking of surface immunoglobulin (Ig) on B cells. Expression of TNF-alpha has been detected in macrophages/monocytes, B-cells, T-cells, basophils, eosinophils, glial cells and mast cells. The effects of TNF-alpha are mediated through the TNF receptors, TNFRI and TNFRII, and this induces a variety of signaling events within cells, leading to necrosis or apoptosis particularly in transformed cells, as well as the induction of inflammatory processes, cell proliferation, cytokine release and activation of T- and B-lymphocytes. Additionally, localized, low-level expression of TNF-alpha participates in tissue re-modeling and host defence responses, including the destruction of tumor cells or virus infected cells and enhancement of antibacterial activities of granulocytes. TNF-alpha is synthesized as a 233 amino acid peptide and is expressed as a 26kDa membrane bound protein. This membrane bound form can be cleaved by TNF alpha converting enzyme (TACE) to produce a soluble 17 kDa protein that associates to form a biologically active homotrimer. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

Arduise, C. et al., J. Immunol.,2008; 181(10): 7002-7013.
Boillot, A. et al.,Clin Intensive Care,1995; 6(2): 52-6.
Dyugovskaya, L. et al., Am J Respir Crit Care Med.,2003; 168(2): 242-9.
Hamacher, J. et al., Am J Respir Crit Care Med., 2002; 166(5): 651-6.
Herve, P. et al., Blood,1992; 79(12): 3362-8.
Herve, P. et al., Bone Marrow Transplant,1991; 7 Suppl 2: 149.
Ida, H. et al., J Immunol.,1997; 159(5): 2154-60.
Limb, G. A. et al.,Am J Pathol.,2002; 160(5): 1847-55.
Marfaing-Koka, A. et al.,Int Immunol.,1996; 8(10): 1587-94.
Mey, A. et al., J Leukoc Biol.,1997; 61(1): 17-23.
Musey, L. et al., J Virol.,2003; 77(1): 291-300.
Racadot, E. et al.,Bone Marrow Transplant,1995; 15(5): 669-77.
van Hensbroek, M. B. et al., J Infect Dis.,1996; 174(5): 1091-7.
Venet, F. et al.,J Immunol.,2006; 177(9): 6540-7.
Voorzanger, N. et al., Cancer Res.,1996; 56(23): 5499-505.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Tumor necrosis factor (TNF-alpha) antibody

Immunohistochemistry analysis of TNF-alpha Antibody (Cat.-No AP20373PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Tumor necrosis factor (TNF-alpha) (1-234) antibody

Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 M1-C4 Purified Hu, Rt E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Tumor necrosis factor (TNF-alpha) antibody

Tumor necrosis factor (TNF-alpha) Mouse IgG1 4C6-H8 Purified Can, Fe, Hu, Ms, Rb, Rt, Ze C, F, ICC/IF, P
0.2 mg / €390.00
  Acris Antibodies GmbH

Tumor necrosis factor (TNF-alpha) antibody

Tumor necrosis factor (TNF-alpha) Mouse IgG1 4C6-H8 Purified Can, Fe, Hu, Ms, Rb, Rt, Ze C, F, ICC/IF, IP, P
0.1 mg / €320.00
  Acris Antibodies GmbH

Tumor necrosis factor (TNF-alpha) antibody

Tumor necrosis factor (TNF-alpha) Mouse IgG1 4C6-H8 Purified Can, Fe, Hu, Ms, Rb, Rt, Ze C, F, ICC/IF, IP, P
20 µg / €160.00
  Acris Antibodies GmbH

Tumor necrosis factor (TNF-alpha) antibody

Tumor necrosis factor (TNF-alpha) Mouse IgG1 SPM543 Purified Can, Fe, Hu, Ms, Rb, Rt, Ze C, F, ICC/IF, IP, P
0.2 mg / €390.00
  Acris Antibodies GmbH

Tumor necrosis factor (TNF-alpha) antibody

Tumor necrosis factor (TNF-alpha) Mouse IgG1 SPM543 Purified Can, Fe, Hu, Ms, Rb, Rt, Ze C, F, ICC/IF, IP, P
0.1 mg / €320.00
  Acris Antibodies GmbH

Tumor necrosis factor (TNF-alpha) antibody

Tumor necrosis factor (TNF-alpha) Mouse IgG1 SPM543 Purified Can, Fe, Hu, Ms, Rb, Rt, Ze C, F, ICC/IF, IP, P
20 µg / €160.00
  Acris Antibodies GmbH

Tumor necrosis factor (TNF-alpha) antibody

Tumor necrosis factor (TNF-alpha) Mouse IgG1 P/T2 Purified Can, Fe, Hu, Ms, Rb, Rt, Ze C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Tumor necrosis factor (TNF-alpha) antibody

Tumor necrosis factor (TNF-alpha) Mouse IgG1 P/T2 Purified Can, Fe, Hu, Ms, Rb, Rt, Ze C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Tumor necrosis factor (TNF-alpha) antibody

Tumor necrosis factor (TNF-alpha) Mouse IgG1 P/T2 Purified Can, Fe, Hu, Ms, Rb, Rt, Ze C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Tumor necrosis factor (TNF-alpha) antibody

Tumor necrosis factor (TNF-alpha) Mouse IgG1 TNF706 Purified Can, Fe, Hu, Ms, Rb, Rt, Ze C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Tumor necrosis factor (TNF-alpha) antibody

Tumor necrosis factor (TNF-alpha) Mouse IgG1 TNF706 Purified Can, Fe, Hu, Ms, Rb, Rt, Ze C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Tumor necrosis factor (TNF-alpha) antibody

Tumor necrosis factor (TNF-alpha) Mouse IgG1 TNF706 Purified Can, Fe, Hu, Ms, Rb, Rt, Ze C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Tumor necrosis factor (TNF-alpha) antibody

Tumor necrosis factor (TNF-alpha) Mouse IgG1 TNF706 + P/T2 Purified Can, Fe, Hu, Ms, Rb, Rt, Ze C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Tumor necrosis factor (TNF-alpha) antibody

Tumor necrosis factor (TNF-alpha) Mouse IgG1 TNF706 + P/T2 Purified Can, Fe, Hu, Ms, Rb, Rt, Ze C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Tumor necrosis factor (TNF-alpha) antibody

Tumor necrosis factor (TNF-alpha) Mouse IgG1 TNF706 + P/T2 Purified Can, Fe, Hu, Ms, Rb, Rt, Ze C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Tumor necrosis factor (TNF-alpha) antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using TNFA antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Tumor necrosis factor (TNF-alpha) antibody

Western blot using Anti-Human TNF-a (RABBIT) Antibody. Membrane blocked in 1% BSA-TBS-T 30 min RT, Rb-a-TNF alpha added at 1:1000 in 1% BSA-TBS-T o/n 4°C, DyLight 649 Gt-a-Rb 611-143-122 added at 1:20,000 in MB-070 30 min RT. Rabbit Serum Hu C, E, FN, P, WB
1 ml / €900.00
  OriGene Technologies, Inc.

Tumor necrosis factor (TNF-alpha) antibody

Western blot using Anti-Human TNF-a (RABBIT) Antibody. Membrane blocked in 1% BSA-TBS-T 30 min RT, Rb-a-TNF alpha added at 1:1000 in 1% BSA-TBS-T o/n 4°C, DyLight 649 Gt-a-Rb 611-143-122 added at 1:20,000 in MB-070 30 min RT. Rabbit Purified Hu C, E, FN, P, WB
1 mg / €900.00
  OriGene Technologies, Inc.

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