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TWEAK / TNFSF12 antibody

Principal name

TWEAK / TNFSF12 antibody

Alternative names for TWEAK / TNFSF12 antibody

APO3 ligand, APO3L, DR3LG, Tumor necrosis factor ligand superfamily member 12, TNF-related weak inducer of apoptosis

SwissProt ID

O43508 (Human), O54907 (Mouse)

Gene ID

407977 (TNFSF12-TNFSF13), 21944 (Tnfsf12), 8742 (TNFSF12)

Ncbi ID

NP_003800, NP_003800.1, 9606, NP_035744, AAH71837.1, O43508, NP_035744.1, NP_742086, NM_003809, NM_011614, NM_172089

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Rt (Rat), Eq (Equine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Functional assay (FN), Flow Cytometry (F), Immunoprecipitation (IP)

Background of TWEAK / TNFSF12 antibody

TNFSF12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Jin,L., et al., (2004) J. Invest. Dermatol. 122 (5), 1175-1179

Immunohistochemistry (IHC) analyzes of TWEAK antibody (Cat.-No.: AP20513PU-N) in paraffin-embedded human brain tissue.

AP20513PU-N

Western blot (WB) analysis of TWEAK antibody (Cat.-No.: AP20513PU-N) in extracts from COLO cells.

AP20513PU-N

Human 721_B; WB Suggested Anti-TNFSF12 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 721_B cell lysate; TNFSF12 antibody - N-terminal region (ARP36558_P050) in Human 721_B cells using Western Blot

ARP36558_P050

Human Muscle; WB Suggested Anti-TNFSF12 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human muscle; TNFSF12 antibody - N-terminal region (AVARP20005_P050) in Human Muscle cells using Western Blot

AVARP20005_P050

Immunohistochemistry analysis of paraffin-embedded human brain, using TNF12 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using TNF12 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from COLO205 cells/HuvEc cells/293 cells, using TNF12 Antibody. The lane on the right is treated with the synthesized peptide.

Western Blot analysis of TNFSF12 expression in transfected 293T cell line (H00008742-T01) by TNFSF12 MaxPab polyclonal antibody.Lane 1: TNFSF12 transfected lysate(14.74 KDa).Lane 2: Non-transfected lysate.

H00008742-B01

H00008742-B01P

Detection limit for recombinant GST tagged TNFSF12 is approximately 0.03ng/ml as a capture antibody.

H00008742-M01

Immunohistochemistry of TNFSF12 in human brain tissue with TNFSF12 polyclonal antibody ( Cat # PAB12960 ) at 10 µg/ml.

PAB12960

Western blot analysis of ( A ) 5 ng, ( B ) 25 ng and ( C ) 50 ng of recombinant TNFSF12 with TNFSF12 polyclonal antibody ( Cat # PAB12960 ) at 1 µg/ml.

PAB12960

Western blot analysis of TWEAK expression in SKNSH (A); WI38 (B); HuvEc (C) whole cell lysates.

Representative Standard Curve

CEK1356

Representative Standard Curve

CEK1569

Flow cytometric analysis of human TWEAK transfected cells using Anti-CD255 Antibody; followed by anti-mouse IgG PE.

TP723463

Western blot analysis of (A) 5 ng, (B) 25 ng and (C) 50 ng of recombinant TWEAK with TWEAK antibody at 1 ug/ml.

TA306313

Immunohistochemistry of TWEAK in human brain tissue with TWEAK antibody at 10 ug/ml.

TA306313

WB Suggested Anti-TNFSF12 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human muscle

TA330020

Western blot analysis of extracts of various cell lines, using TNFSF12 antibody.

TA332822

Western blot analysis of extracts from COLO cells, HUVEC cells and 293 cells, using TNF12 antibody.The lane on the right is treated with the synthesized peptide.

TA314806

Immunohistochemistry analysis of paraffin-embedded human brain tissue using TNF12 antibody.The picture on the right is treated with the synthesized peptide.

TA314806

Immunofluorescence analysis of HeLa cells, using TNF12 antibody.The picture on the right is treated with the synthesized peptide.

TA314806

To detect hTWEAK by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hTWEAK.

TA328466

To detect hTWEAK by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TWEAK (TA328467) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTWEAK.

TA328466

To detect hTWEAK by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TWEAK (TA328466) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTWEAK.

TA328467

WB Suggested Anti-TNFSF12 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: 721_B cell lysate

TA341640

Western blot analysis on HuvEc,Jurkat and 293 cell lysate using TNFSF12 Antibody

TA347537

Human TWEAK transfected cells stained with purified Carl-2, followed by biotinylated anti-mouse IgG and Sav-PE

TA352383

Human TWEAK transfected cells stained with Carl-1 PE

TA352382

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA306313	TWEAK / TNFSF12 antibody
	Rabbit Polyclonal TWEAK Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P, WB	0.1 mg / €415.00
						OriGene Technologies, Inc.
+1 additional image
TA314806	TWEAK / TNFSF12 antibody
	Rabbit polyclonal anti-TNF12 antibody
	Rabbit	IgG	Purified	Hu, Ms	E, ICC/IF, P, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
+2 additional images
TA328466	TWEAK / TNFSF12 antibody
	Biotinylated Anti-Human TWEAK Goat Polyclonal Antibody
	Goat	IgG	Purified	Hu	E, WB	50 µg / €275.00
						OriGene Technologies, Inc.
+1 additional image
TA328467	TWEAK / TNFSF12 antibody
	Anti-Human TWEAK Goat Polyclonal Antibody
	Goat	IgG	Purified	Hu	E, WB	0.1 mg / €275.00
						OriGene Technologies, Inc.
TA330020	TWEAK / TNFSF12 antibody
	Rabbit Polyclonal Anti-TNFSF12 Antibody
	Rabbit	IgG	Purified	Hu	WB	0.1 ml / €360.00
						OriGene Technologies, Inc.
TA332822	TWEAK / TNFSF12 antibody
	Rabbit anti-TNFSF12 Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt		0.1 mg / €335.00
						OriGene Technologies, Inc.
TA341640	TWEAK / TNFSF12 antibody
	Rabbit Polyclonal Anti-TNFSF12 Antibody
	Rabbit	IgG	Purified	Can, Eq, Hu, Ms, Rb, Rt, Ze	WB	0.1 ml / €360.00
						OriGene Technologies, Inc.
TA347537	TWEAK / TNFSF12 antibody
	Rabbit Polyclonal Anti-TNFSF12 Antibody
	Rabbit	IgG	Purified	Hu, Ms	WB	0.1 mg / €335.00
						OriGene Technologies, Inc.
AP20513PU-N	TWEAK / TNFSF12 antibody
	Rabbit		Aff - Purified	Hu, Ms	ICC/IF, P, WB	0.1 mg / €370.00
						OriGene Technologies GmbH
+1 additional image
PP1124B1	TWEAK / TNFSF12 antibody
	Goat		Biotin	Hu	E, WB	25 µg / €250.00
						OriGene Technologies GmbH
PP1124B2	TWEAK / TNFSF12 antibody
	Goat		Biotin	Hu	E, WB	50 µg / €340.00
						OriGene Technologies GmbH
PP1124P1	TWEAK / TNFSF12 antibody
	Goat		Aff - Purified	Hu	E, FN, WB	50 µg / €250.00
						OriGene Technologies GmbH
PP1124P2	TWEAK / TNFSF12 antibody
	Goat		Aff - Purified	Hu	E, FN, WB	0.1 mg / €340.00
						OriGene Technologies GmbH