TWIST1 antibody
Principal name
TWIST1 antibody
Alternative names for TWIST1 antibody
Twist-related protein 1, TWIST, BHLHA38, Class A basic helix-loop-helix protein 38
Ncbi ID
9606, NP_000465, NP_000465.1, NM_000474, NP_035788.1, NM_011658
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Paraffin Sections (P)
Background of TWIST1 antibody
- Kit Component:
- - KN202920G1, TWIST1 gRNA vector 1 in pCas-Guide vector
- KN202920G2, TWIST1 gRNA vector 2 in pCas-Guide vector
- KN202920D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Reinhold,M.I., et al., (2006) J. Biol. Chem. 281 (3), 1381-1388
Western blot analysis of TWIST1 over-expressed 293 cell line, cotransfected with TWIST1 Validated Chimera RNAi ( Cat # H00007291-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TWIST1 monoclonal antibody (M01), clone 3E11 (Cat # H00007291-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of TWIST1 expression in transfected 293T cell line by TWIST1 monoclonal antibody (M01), clone 3E11.Lane 1: TWIST1 transfected lysate(21 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged TWIST1 is approximately 0.3ng/ml as a capture antibody.
Western Blot analysis of TWIST1 expression in transfected 293T cell line by TWIST1 monoclonal antibody (M03), clone 2F8.Lane 1: TWIST1 transfected lysate(21 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged TWIST1 is approximately 0.3ng/ml as a capture antibody.
Human Uterus; Uterus; TWIST1 antibody - C-terminal region (ARP37997_T100) in Human Uterus cells using Immunohistochemistry
Jurkat; Host: Rabbit. Target Name:TWIST1. Sample Tissue:Jurkat. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:2.5ug/mL. Peptide Concentration: 2.0ug/mL. Lysate Quantity: 25ug/lane. Gel Concentration: 12%.; TWIST1 antibody - C-terminal region (ARP37997_T100) in Jurkat cells using Western Blot
Human Jurkat; WB Suggested Anti-TWIST1 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; TWIST1 antibody - C-terminal region (ARP37997_T100) in Human Jurkat cells using Western Blot
Immunofluorescence of purified MaxPab antibody to TWIST1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of TWIST1 expression in transfected 293T cell line (H00007291-T01) by TWIST1 MaxPab polyclonal antibody.Lane1:TWIST1 transfected lysate(22.22 KDa).Lane2:Non-transfected lysate.
Western Blot analysis of TWIST1 expression in transfected 293T cell line (H00007291-T03) by TWIST1 MaxPab polyclonal antibody.Lane 1: TWIST1 transfected lysate(21.00 KDa).Lane 2: Non-transfected lysate.
TWIST1 MaxPab rabbit polyclonal antibody. Western Blot analysis of TWIST1 expression in HeLa.
Placenta stained by Twist-1 antibody Cat.-No AP55360PU-N in Immunohistochemistry on Paraffin Sections
Western blot analysis of TWIST expression in Jurkat (A); Raw264.7 (B); rat liver (C) whole cell lysates.
Predicted cell location: Nucleus. Positive control: Human cervical cancer tissue . Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using TWIST1 antibody at dilution 1/15, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Nucleus. Positive control: Human colon and liver cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using TWIST1 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Nucleus. Positive control: Human colon and liver cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using TWIST1 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200)
Western blot analysis of extracts of HL60 cell line, using TWIST1 antibody.
Host: Rabbit; Target Name: TWIST1; Sample Tissue: Uterus Tumor lysates; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-TWIST1 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Jurkat cell lysate
Host: Rabbit; Target Name: TWIST1; Sample Tissue: Jurkat; Lane A: Primary Antibody; Lane B: Primary Antibody + Blocking Peptide; Primary Antibody Concentration: 2.5 ug/mL; Peptide Concentration: 2.0 ug/mL; Lysate Quantity: 25 ug/lane; Gel Concentration: 1
Uterus
Western blot analysis of twist expression in Jurkat cells
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA323649 | TWIST1 antibody |
||||||
| Anti-TWIST1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA323650 | TWIST1 antibody |
||||||
| Anti-TWIST1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA335179 | TWIST1 antibody |
||||||
| Rabbit Polyclonal Anti-TWIST1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Eq, Hu, Ms, Por, Rt, Ze | P, WB |
0.1 mg /
€300.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
