TXNL1 antibody
Principal name
TXNL1 antibody
Alternative names for TXNL1 antibody
Thioredoxin-like protein 1, TXL, TXNL, TRP32, 32 kDa thioredoxin-related protein
Ncbi ID
9606, NP_004777, NP_004777.1, NP_058072.2, NM_080887, NM_004786
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of TXNL1 antibody
- Kit Component:
- - KN318518G1, Txnl1 gRNA vector 1 in pCas-Guide vector
- KN318518G2, Txnl1 gRNA vector 2 in pCas-Guide vector
- KN318518D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Zhou R., et al. (2010) Nat Immunol. 11(2):136-40.
Patwari P., et al. (2009) J Biol Chem. 284(37):24996-5003.
TXNL1 monoclonal antibody (M01), clone 1A10 Western Blot analysis of TXNL1 expression in HL-60 ( Cat # L014V1 ).
Detection limit for recombinant GST tagged TXNL1 is approximately 1ng/ml as a capture antibody.
The TXNL1 polyclonal antibody ( Cat # PAB2464 ) is used in Western blot to detect TXNL1 in HL-60 cell lysate.
Mouse cerebellar cortex showing molecular cell layer (mc), Purkinje cells (Pc) and granular cell layer. Trxl-1 antibody (Cat.#AP11335PU-N) gives strong nuclear labeling in Purkinje cells and granular cells with lower cytoplasmic staining. The results correspond precisely to TrxL-1 mRNA expression shown with in situ hybridization (Jimenez A et al. FEBS Lett. 2006, 580(3):960-7) and could be abolished with peptide used for immunization (Blocking peptide). The fixation was 4% paraformaldehyde perfusion (4mins) and 60 mins immersion. Staining was done in frozen sections with Vector Elite kit and nickel intensified DAB as a chromogen. Data courtesy of Prof. Markku Pelto-Huikko, Department of Developmental Biology, Medical School,Tampere University, Finland.
Western blot analysis using anti-TrxL Pab (Cat.#AP11335PU-N) to detect TrxL in HL-60 cell lysate.
Recombinant human TXNL1, 1-289 aa, His-tagged
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-TXNL1 rabbit polyclonal antibody. (TA590620)
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-TXNL1 rabbit polyclonal antibody. (TA590620)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TXNL1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TXNL1 (RC200344, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TXNL1.
ELISA: TXNL1 Antibody - Affinity Purified
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300344 | TXNL1 (transcript variant 1) |
||||
| Recombinant protein of human thioredoxin-like 1 (TXNL1), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
