Tyrosinase (TYR) antibody

Principal name

Tyrosinase (TYR) antibody

Alternative names for Tyrosinase (TYR) antibody

Monophenol monooxygenase, Tumor rejection antigen AB, SK29-AB, LB24-AB

SwissProt ID

O42713 (Agabi), P11344 (Mouse), P14679 (Human), P54834 (Canfa), P55024 (Chick), P55033 (Felca), Q8MIU0 (Bovin)

Gene ID

7299 (TYR), 6872994 (B5FUB9), 22173 (Tyr)

Available reactivities

Hu (Human), Can (Canine), Fe (Feline), Ms (Mouse), Rt (Rat), Eq (Equine), Gt (Goat), Por (Porcine), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Frozen Sections (C)

Background of Tyrosinase (TYR) antibody

Tyrosinase (Tyr) is a trans-membrane glycoprotein that belongs to the type 3 copper protein family (Yin, 2011). It plays a key role in the melanosynthetic pathway and is required for the synthesis of both types of melanin, eumelanin and pheomelanin (Hu, 2011). Tyrosinase is the rate limiting enzyme catalyzing the first two steps in the melanin biosynthesis, converting tyrosine to L-dihydroxy-phenylalanine (DOPA) and subsequently to DOPAquinone (K, 2013). The pigmentation of skin, the browning of vegetables, wound healing and cuticle formation in insects are some of the major responsibilities performed by tyrosinase (Yin, 2011). This melanocyte-specific enzyme is localized to the post-Golgi compartment termed the melanosome (Francis, 2003). Tyrosinase consists of 533 amino acids along with 7 occupied N-glycosylation sites, 17 cysteine residues grouped in 2 cysteine-rich domains, 2 copper binding domains, and 1 C-terminal TMD (Popescu, 2006). It folds in the ER and is transported to the trans-Golgi network where two copper ions are incorporated and performs different catalytic reactions such as the hydroxylation of monophenols to o-diphenols (cresolase activity) and the oxidation of o-diphenols to o-quinones (catechol oxidase activity) (Yin, 2011) . From here it continues its journey to the melanosomes where it initiates the melanin synthesis. This protein is related with severe skin diseases such as type 1 albinism and melanoma and an important target for anti-melanoma vaccine therapies (Popescu, 2006 and Han, 2007). Anti-tyrosinase antibodies may be applied for immunotherapy in patients with malignant melanoma (Merimsky, 1998).

General readings

Bidinost,C., (2006) Invest. Ophthalmol. Vis. Sci. 47 (4), 1486-1490

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Tyrosinase (TYR) (C-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue using Tyrosinase Antibody (C-term) Cat.-No AP17815PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of Tyrosinase Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Purified Hu F, ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Tyrosinase (TYR) antibody

Formalin-Fixed, Paraffin-Embedded Human melanoma (10X) stained with Tyrosinase Antibody Cat.-No AM32831PU (Clone T311) using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining. Mouse IgG2a T311 Purified Hu C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Tyrosinase (TYR) antibody

Formalin-Fixed, Paraffin-Embedded Human melanoma (10X) stained with Tyrosinase Antibody Cat.-No AM32831PU (Clone T311) using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining. Mouse IgG2a T311 Purified Hu C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Tyrosinase (TYR) antibody

Formalin-Fixed, Paraffin-Embedded Human melanoma (10X) stained with Tyrosinase Antibody Cat.-No AM32831PU (Clone T311) using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining. Mouse IgG2a T311 Purified Hu C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Tyrosinase (TYR) antibody

Formalin-paraffin melanoma (20X) stained with Tyrosinase Antibody Cat.-No AM50181PU (Clone SPM360). Mouse IgG2a SPM360 Purified Hu C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Tyrosinase (TYR) antibody

Formalin-paraffin melanoma (20X) stained with Tyrosinase Antibody Cat.-No AM50181PU (Clone SPM360). Mouse IgG2a SPM360 Purified Hu C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Tyrosinase (TYR) antibody

Formalin-paraffin melanoma (20X) stained with Tyrosinase Antibody Cat.-No AM50181PU (Clone SPM360). Mouse IgG2a SPM360 Purified Hu C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Tyrosinase (TYR) antibody

Western blot analysis of extracts from COS7 cells, treated with UV (30mins), using Tyrosinase antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Tyrosinase (TYR) antibody

Predicted band size: 60 kDa . Positive control: A375, Hela and Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: A375 cell lysate Lane2: Hela cell lysate Lane3: Jurkat cell lysate Lysates: 50 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Tyrosinase (TYR) antibody

Predicted band size: 60 kDa . Positive control: A375, Hela and Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: A375 cell lysate Lane2: Hela cell lysate Lane3: Jurkat cell lysate Lysates: 50 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Tyrosinase (TYR) antibody

Western blot analysis of HepG2 cell lysate using TYR antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Tyrosinase (TYR) antibody

WB Suggested Anti-TYR Antibody Titration: 1.25 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Can, Eq, Gt, Hu, Ms, Por, Rt, Sh WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Tyrosinase (TYR) antibody

Western blot analysis on COS7 cell lysate using Tyrosinase Antibody Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

Tyrosinase (TYR) antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYR (RC221797, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYR. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Tyrosinase (TYR) antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TYR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801362) Mouse IgG1 OTI1F3 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Tyrosinase (TYR) (1-433) antibody

Skin: Formalin-Fixed Paraffin-Embedded (FFPE) Mouse IgG2a 0.N.596 Purified Can, Fe, Hu E, ICC/IF, P, WB
0.125 ml / €470.00
  Acris Antibodies GmbH

Tyrosinase (TYR) antibody

Western blot analysis of Tyrosinase Antibody (Cat.-No AP06471PU-N) in extracts from COS7 cells treated with UV 30'. Rabbit Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Tyrosinase (TYR) antibody

Tyrosinase (TYR) Rabbit Serum Ms C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

Tyrosinase (TYR) antibody

Western Blot analysis of TYR expression in transfected 293T cell line (H00007299-T01) by TYR MaxPab polyclonal antibody.

Lane 1: TYR transfected lysate(41.47 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Tyrosinase (TYR) antibody

Western Blot analysis of TYR expression in transfected 293T cell line (H00007299-T01) by TYR MaxPab polyclonal antibody.

Lane 1: TYR transfected lysate(41.47 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

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