Tyrosine-protein kinase JAK2 antibody

Principal name

Tyrosine-protein kinase JAK2 antibody

Alternative names for Tyrosine-protein kinase JAK2 antibody

Janus kinase 2, JAK-2

SwissProt ID

O19064 (Pig), O60674 (Human), Q62120 (Mouse), Q62689 (Rat), Q75R65 (Chick)

Gene ID

3717 (JAK2), 16452 (Jak2), 4893 (NRAS)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Eq (Equine), Op (Opossum), Por (Porcine), Chk (Chicken), Ze (Zebrafish), Can (Canine), Mky (Monkey), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Dot blot (Dot), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C)

Background of Tyrosine-protein kinase JAK2 antibody

JAK2 (Janus Kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to CIS elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor. Among a variety of sites, Tyrosines 1007 and 1008 are sites of trans- or autophosphorylation in vivo and in in vitro kinase reactions.

General readings

Frank, G.D., et al. (2003) Distinct mechanisms of receptor and nonreceptor tyrosine kinase activation by reactive oxygen species in vascular smooth muscle cells: role of metalloprotease and protein kinase C-delta. Mol. Cell. Biol. 23(5):1581-1589.
Kaszubska, W., et al. (2002) Protein tyrosine phosphatase 1B negatively regulates leptin signaling in a hypothalamic cell line. Mol. Cell. Endocrinol. 195(1-2):109-118.
Kirito, K., et al. (2002) Thrombopoietin regulates Bcl-xL gene expression through Stat5 and phosphatidylinositol 3-kinase activation pathways. J. Biol. Chem. 277(10):8329-8337.
Shaw, S., et al. (2002) Janus kinase 2, an early target of alpha 7 nicotinic acetylcholine receptor-mediated neuroprotection against Abeta-(1-42) amyloid. J. Biol. Chem. 277(47):44920-44924.
Sutherland, C.L., et al. (2002) UL16-binding proteins, novel MHC class I-related proteins, bind to NKG2D and activate multiple signaling pathways in primary NK cells. J. Immunol. 168(2):671-679.
Zeng, Z.Z., et al. (2002) 5(S)-hydroxyeicosatetraenoic acid stimulates DNA synthesis in human microvascular endothelial cells via activation of Jak/STAT and phosphatidylinositol 3-kinase/Akt signaling, leading to induction of expression of basic fibroblast growth factor 2. J. Biol. Chem. 277(43):41213-41219.
Eguchi, S., et al. (2001) Activation of MAPKs by angiotensin II in vascular smooth muscle cells. Metalloprotease-dependent EGF receptor activation is required for activation of ERK and p38 MAPK but not for JNK. J. Biol. Chem. 276(11):7957-7962.
Madamanchi, N.R., et al. (2001) Thrombin regulates vascular smooth muscle cell growth and heat shock proteins via the JAK-STAT pathway. J. Biol. Chem. 276(22):18915-18924.
Myers, M.P., et al. (2001) TYK2 and JAK2 are substrates of protein-tyrosine phosphatase 1B. J. Biol. Chem. 276(51):47771-47774.
Madamanchi, N.R., et al. (2000) Reactive oxygen species regulate heat shock protein 70 via the JAK/STAT Pathway. Arteriocher. Thromb. Vasc. Biol. 21(3):321-326.
Amiri, F., et al. (1999) Hyperglycemia enhances angiotensin II-induced janus-activated kinase/STAT signaling in vascular smooth muscle cells. J. Biol. Chem. 274(45):32382-32386.
Ihle, J.N. (1996) STATs: signal transducers and activators of transcription. Cell 84(3):331-334.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Tyrosine-protein kinase JAK2 antibody

Western blot analysis of extracts from HT-29 cells, using JAK2 (Ab-1007) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Tyrosine-protein kinase JAK2 antibody

Western blot analysis of extracts from K562 cells, using JAK2 (Ab-221) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Tyrosine-protein kinase JAK2 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue using JAK2 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Tyrosine-protein kinase JAK2 antibody

Western blot analysis of extracts from 293 cells, treated with etoposide (25uM, 24hours), using JAK2 (Ab-570) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Tyrosine-protein kinase JAK2 antibody

Predicted cell location: Cytoplasm. Positive control: Human brain and lung cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded Human brain and lung cancer tissue using JAK2 antibody at dilution 1/35, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Tyrosine-protein kinase JAK2 antibody

Predicted cell location: Cytoplasm. Positive control: Human lung and ovarian cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human lung and ovarian cancer tissue using JAK2 antibody at dilution 1/10, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Tyrosine-protein kinase JAK2 antibody

Western blot analysis of JAK2 expression in HT29 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Tyrosine-protein kinase JAK2 antibody

Western blot analysis of JAK2 expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Tyrosine-protein kinase JAK2 antibody

Western blot analysis of JAK2 expression in etoposide treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Tyrosine-protein kinase JAK2 antibody

Western blot analysis of extracts of various cell lines, using JAK2 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Tyrosine-protein kinase JAK2 antibody

Western blot analysis of JAK2 in HeLa cell lysate with JAK2 antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Tyrosine-protein kinase JAK2 antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-JAK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA806794)(1:150) Mouse IgG2b OTI7D12 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Tyrosine-protein kinase JAK2 antibody

Western blot analysis using JAK2 mouse mAb against THP-1(1) cell lysate. Mouse IgG1 1C1 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

Tyrosine-protein kinase JAK2 pTyr1007 antibody

Immunohistochemistry (IHC) analysis of JAK2 pTyr1007 antibody (Cat.-No.: aP01621PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €350.00
  Acris Antibodies GmbH

Tyrosine-protein kinase JAK2 pTyr1007 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using JAK2 Antibody (Phospho-Tyr1007) Cat.-No AP02435PU. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

Tyrosine-protein kinase JAK2 pTyr1007 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using JAK2 Antibody (Phospho-Tyr1007) Cat.-No AP02435PU. Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µl / €240.00
  Acris Antibodies GmbH

Tyrosine-protein kinase JAK2 (C-term) antibody

Western blot using JAK2 antibody (AP05273PU-N) on NIH3T3 cells stimulated with PDGF for 10 minutes. Antibody
used at 1 µg/ml and incubated for 2 hours at RT. Detected using goat antirabbit IgG conjugated to HRP. Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €710.00
  Acris Antibodies GmbH

Tyrosine-protein kinase JAK2 antibody

Immunohistochemistry (IHC) analyzes of JAK2 antibody (Cat.-No.: AP06189PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Tyrosine-protein kinase JAK2 antibody

Figure 1. Western blot analysis of extracts from mouse brain tissue, using JAK2 Antibody AP08050PU (Lane 1 and 2) and JAK2 (phospho-Tyr1007) antibody AP02435PU (Lane 3 and 4). Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Acris Antibodies GmbH

Tyrosine-protein kinase JAK2 antibody

Figure 1. Western blot analysis of extracts from mouse brain tissue, using JAK2 Antibody AP08050PU (Lane 1 and 2) and JAK2 (phospho-Tyr1007) antibody AP02435PU (Lane 3 and 4). Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €230.00
  Acris Antibodies GmbH

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