TYRP2 / DCT antibody

Principal name

TYRP2 / DCT antibody

Alternative names for TYRP2 / DCT antibody

Tyrosinase-related protein 2, TRP2, TRP-2, L-dopachrome tautomerase, L-dopachrome Delta-isomerase

SwissProt ID

O93505 (Chick), P29812 (Mouse), P40126 (Human), Q4R1H1 (Pig), Q95119 (Bovin)

Gene ID

1638 (DCT), 13190 (Dct)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of TYRP2 / DCT antibody

Tyrosinase (TYR), a type I membrane protein and copper-containing enzyme,is involved in the production of melanin, the primary pigment found in vertebrates.Melanin biogenesis requires the enzymatic activity of TYR, which catalyzes the critical and rate-limiting step of tyrosine hydroxylation in the biosynthesis of melanin. Defects effecting TYR activity result in various forms of albinism. The TYR-related proteins, TRP1 and TRP2, are also specifically expressed in melanocytes, and they likewise contribute to the synthesis of melanin within the melanosomes. The TRPs, including TYR, all share a similar transmembrane region, contain two metal-binding regions and a cysteinerich epidermal growth factor motif, and are localized in the melanosomal membrane. These proteins, however, have distinct catalytic activity, and they individually contribute to the biosynthesis of melanin biopolymers. The TRPs are believed to exist as a multi-enzyme complex, as these proteins form aggregates together, and the expression of TRP1 also helps stabilize TYR in melanocytes.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TYRP2 / DCT antibody

  Rabbit polyclonal anti-DCT (dopachrome tautomerase) antibody  
Western blot analysis of extracts from K562 cells, using DCT antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TYRP2 / DCT antibody

  Anti-DCT Rabbit Polyclonal Antibody  
Predicted band size: 59 kDa . Positive control: Human liver cancer and fetal liver tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Human liver cancer tissue lysate Lane2: Human fetal liver tissue lysate Lysates: 50 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TYRP2 / DCT antibody

  Anti-DCT Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human colon and cervical cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using DCT antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TYRP2 / DCT antibody

Western blot (WB) analysis of TRP2 antibody (Cat.-No. AP01482PU-N) in extracts from K562 cells. Rabbit Aff - Purified Hu, Ms ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TYRP2 / DCT (N-term) antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DCT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TYRP2 / DCT (Center) antibody

Western blot analysis of DCT Antibody Cat.-No AP51203PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the DCT antibody detected the DCT protein (arrow) Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TYRP2 / DCT (N-term) antibody

Formalin fixed, paraffin embedded breast carcinoma stained with DCT Antibody (N-term) Cat.-No AP51204PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TYRP2 / DCT antibody

Western Blot analysis of DCT expression in transfected 293T cell line (H00001638-T03) by DCT MaxPab polyclonal antibody.

Lane 1: DCT transfected lysate(59.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

TYRP2 / DCT antibody

Immunofluorescence analysis of A549 cells, using DCT Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
50 µg / €230.00
  Assay Biotech

TYRP2 / DCT antibody

Immunofluorescence analysis of A549 cells, using DCT Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €330.00
  Assay Biotech

TYRP2 / DCT antibody

Immunohistochemical analysis of TRP2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

TYRP2 / DCT antibody

Immunohistochemical analysis of TRP2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

TYRP2 / DCT antibody

Immunohistochemical analysis of TRP2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CRISPR

Catalog No. Species Donor Vector  

DCT - human gene knockout kit via CRISPR

DCT - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

DCT - human gene knockout kit via CRISPR

DCT - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DCT - human gene knockout kit via CRISPR

DCT - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DCT - human gene knockout kit via CRISPR

DCT - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dct - mouse gene knockout kit via CRISPR

Dct - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Dct - mouse gene knockout kit via CRISPR

Dct - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dct - mouse gene knockout kit via CRISPR

Dct - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

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