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UBC9 / UBE2I antibody

Principal name

UBC9 / UBE2I antibody

Alternative names for UBC9 / UBE2I antibody

UBCE9, Ube2i, Ubce2i, SUMO-protein ligase, SUMO-conjugating enzyme UBC9, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, Ubiquitin carrier protein I, Ubiquitin carrier protein 9

SwissProt ID

P63279 (Human), P63280 (Mouse), P63281 (Rat), P63282 (Xenla), P63283 (Chick)

Gene ID

7329 (UBE2I), 22196 (Ube2i), 25573

Ncbi ID

NP_003336.1, 9606, NP_003336, NM_194260, NM_194261, NP_919237, NP_919235, NP_919236, NM_011665, NM_001177610, NM_013050, NM_003345, NP_001171080.1

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Mky (Monkey)

Available hosts

Rabbit, Goat, Mouse

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of UBC9 / UBE2I antibody

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2I is a member of the E2 ubiquitin-conjugating enzyme family.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

General readings

1. Wang ZY, Qiu QQ, Seufert W, Taguchi T, Testa JR, Whitmore SA, Callen DF, Welsh D, Shenk T, Deuel TF. Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9. J Biol Chem. 1996 Oct 4;271(40):24811-6. PMID: 8798754.
2. Subramaniam S, Mealer RG, Sixt KM, Barrow RK, Usiello A, Snyder SH. Rhes, a physiologic regulator of sumoylation, enhances cross-sumoylation between the basic sumoylation enzymes E1 and Ubc9. J Biol Chem. 2010 Jul 2;285(27):20428-32. PMID: 20424159 (in WB on Mouse Brain).
3. Ihara M, Stein P, Schultz RM. UBE2I (UBC9), a SUMO-conjugating enzyme, localizes to nuclear speckles and stimulates transcription in mouse oocytes.
Biol Reprod. 2008 Nov;79(5):906-13. PMID: 18703419.

UBE2I polyclonal antibody ( Cat # PAB6303 ) staining ( 0.3 µg/ml ) of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

PAB6303

UBE2I polyclonal antibody ( Cat # PAB6303 ) staining ( 0.1 µg/ml ) of human kidney lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.

PAB6303

Human Jurkat; WB Suggested Anti-UBE2I Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate. There is BioGPS gene expression data showing that UBE2I is expressed in Jurkat.; UBE2I antibody - N-terminal region (ARP43021_T100) in Human Jurkat cells using Western Blot

ARP43021_T100

Human Jurkat; WB Suggested Anti-UBE2I Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate. There is BioGPS gene expression data showing that UBE2I is expressed in Jurkat.; UBE2I antibody - middle region (ARP43022_T100) in Human Jurkat cells using Western Blot

ARP43022_T100

Figure 1. (LEFT) Western blot analysis of anti-UBC9 Antibody (Cat. #AM11046PU-N) in Ramos cell line lysates (35ug/lane). UBC9(arrow) was detected using the purified Mab. (RIGHT) Western blot analysis of anti-UBC9 Antibody (C-term) (Cat. #AM11046PU-N) in mouse spleen tissue lysates (35ug/lane). UBC9(arrow) was detected using the purified Mab.

AM11046PU-N

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

AP11077PU-N

Western blot analysis of anti-UBE2I Antibody (N-term) (Cat.#AP11077PU-N) in A2058 cell line lysates (35ug/lane). UBE2I(arrow) was detected using the purified Pab.

AP11077PU-N

Immunohistochemical analysis of paraffin-embedded prostate tissues using UBE2I mouse mAb with DAB staining.

AM06691SU-N

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using UBE2I mouse mAb with DAB staining.

AM06691SU-N

Immunofluorescence analysis of HepG2 cells using UBE2I mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

AM06691SU-N

Western blot analysis using UBE2I mAb against human UBE2I (AA: 1-158) recombinant protein. (Expected MW is 45.3 kDa)

AM06691SU-N

Western blot analysis using UBE2I mouse mAb against Hela (1), HepG2 (2), and Cos7 (3) cell lysate.

AM06691SU-N

Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)

AM06691SU-N

Flow cytometric analysis of HepG2 cells using UBE2I mouse mAb (green) and negative control (red).

AM06691SU-N

Western blot analysis of UBC9 expression in HeLa (A); A431 (B); Raw264.7 (C); PC12 (D) whole cell lysates.

TP301199

TP317884

TP317945

TP324182

TA302696 (0.1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA302696

Western blot analysis of extracts of various cell lines, using UBE2I antibody.

TA327329

Immunohistochemistry of paraffin-embedded human breast cancer using UBE2I antibody at dilution of 1:200 (400x lens).

TA327329

Immunohistochemistry of paraffin-embedded human esophageal cancer using UBE2I antibody at dilution of 1:200 (400x lens).

TA327329

Immunofluorescence analysis of U2OS cell using UBE2I antibody. Blue: DAPI for nuclear staining.

TA327329

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA327329	UBC9 / UBE2I antibody
	UBE2I Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt		0.1 mg / €335.00
							OriGene Technologies, Inc.
+3 additional images	Highly validated antibody!
AM06691SU-N	UBC9 / UBE2I antibody
	Mouse	IgG1	1B10	Ascites	Hu, Mky	E, F, ICC/IF, P, WB	0.1 ml / €430.00
							OriGene Technologies GmbH
+6 additional images	Highly validated antibody!
TA302696	UBC9 / UBE2I (N-term) antibody
	Goat Polyclonal Antibody against UBE2I
	Goat			Aff - Purified	Can, Hu, Ms, Rt	E, ICC/IF, P, WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
AM11046PU-N	UBC9 / UBE2I antibody
	Mouse	IgG1	67AT1273.95.90	Purified	Hu, Ms	WB	0.4 ml / €370.00
							OriGene Technologies GmbH
AP11077PU-N	UBC9 / UBE2I (N-term) antibody
	Rabbit	Ig		Purified	Hu	P, WB	0.4 ml / €370.00
							OriGene Technologies GmbH
+1 additional image

CRISPR

Catalog No.	Species	Donor Vector
KN201199	UBE2I - human gene knockout kit via CRISPR
	Human	GFP-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN201199BN	UBE2I - human gene knockout kit via CRISPR
	Human	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN201199LP	UBE2I - human gene knockout kit via CRISPR
	Human	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN201199RB	UBE2I - human gene knockout kit via CRISPR
	Human	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No.	Species	Pres.	Purity	Source
TP301199	UBC9 / UBE2I (transcript variant 4)
	Recombinant protein of human ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) (UBE2I), transcript variant 4
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €680.00
					OriGene Technologies, Inc.

TP317884	UBC9 / UBE2I (transcript variant 1)
	Recombinant protein of human ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) (UBE2I), transcript variant 1
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €680.00
					OriGene Technologies, Inc.

TP317945	UBC9 / UBE2I (transcript variant 2)
	Recombinant protein of human ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) (UBE2I), transcript variant 2
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €438.00
					OriGene Technologies, Inc.

TP324182	UBC9 / UBE2I (transcript variant 3)
	Recombinant protein of human ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) (UBE2I), transcript variant 3
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €680.00
					OriGene Technologies, Inc.

SA6046	UBC9 / UBE2I
	Human	Purified	> 95 % by SDS-PAGE	E. coli	0.1 mg / €230.00
					OriGene Technologies GmbH
+1 additional image
SA6046X	UBC9 / UBE2I
	Human	Purified	> 95 % by SDS-PAGE	E. coli	0.5 mg / €540.00
					OriGene Technologies GmbH
+1 additional image

Lysates

Catalog No.
LY405115	UBE2I overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.
LY405116	UBE2I overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.
LY430673	UBE2I overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.
LY430674	UBE2I overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.
RC217884L2V	Lenti ORF particles, UBE2I (mGFP-tagged) - Human ubiquitin-conjugating enzyme E2I (UBE2I), transcript variant 1, 200 uL, >10^7 TU/mL
		200 µl / €907.00
		OriGene Technologies, Inc.

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