UBC9 / UBE2I antibody
Principal name
UBC9 / UBE2I antibody
Alternative names for UBC9 / UBE2I antibody
UBCE9, Ube2i, Ubce2i, SUMO-protein ligase, SUMO-conjugating enzyme UBC9, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, Ubiquitin carrier protein I, Ubiquitin carrier protein 9
Gene ID
7329 (UBE2I)
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Flow Cytometry (F)
Background of UBC9 / UBE2I antibody
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. Catalyzes the covalent attachment of ubiquitin-like protein SUMO-1 to other proteins. Forms a tight complex in association with two RAN GTPase interacting proteins, RANGAP1 and RANBP2. Interacts with E1A and E2A. Interacts with HIPK1 and HIPK2. Interacts with SIAH1 and PARP. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
General readings
1. Wang ZY, Qiu QQ, Seufert W, Taguchi T, Testa JR, Whitmore SA, Callen DF, Welsh D, Shenk T, Deuel TF. Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9. J Biol Chem. 1996 Oct 4;271(40):24811-6. PMID: 8798754.
2. Subramaniam S, Mealer RG, Sixt KM, Barrow RK, Usiello A, Snyder SH. Rhes, a physiologic regulator of sumoylation, enhances cross-sumoylation between the basic sumoylation enzymes E1 and Ubc9. J Biol Chem. 2010 Jul 2;285(27):20428-32. PMID: 20424159 (in WB on Mouse Brain).
3. Ihara M, Stein P, Schultz RM. UBE2I (UBC9), a SUMO-conjugating enzyme, localizes to nuclear speckles and stimulates transcription in mouse oocytes.
Biol Reprod. 2008 Nov;79(5):906-13. PMID: 18703419.
Immunohistochemical of paraffin-embedded human lung cancer using 10070-1-AP(UBC9 antibody) at dilution of 1:50 (under 10x lens)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10070-1-AP(UBC9 antibody) at dilution of 1:300
Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10070-1-AP(UBC9 antibody) at dilution of 1:200
Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10070-1-AP(UBC9 antibody) at dilution of 1:300
Mouse testis tissue were subjected to SDS PAGE followed by western blot with 10070-1-AP(UBC9 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human lung cancer using 10224-1-AP(UBC9 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using 10224-1-AP(UBC9 antibody) at dilution of 1:50 (under 40x lens)
Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10224-1-AP(UBC9 antibody) at dilution of 1:50
Human testis tissue were subjected to SDS PAGE followed by western blot with 10224-1-AP(UBC9 antibody) at dilution of 1:400
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10224-1-AP(UBC9 antibody) at dilution of 1:400
Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10224-1-AP(UBC9 antibody) at dilution of 1:300
Jurkat cells were subjected to SDS PAGE followed by western blot with 10224-1-AP(UBC9 antibody) at dilution of 1:50
Jurkat cells were subjected to SDS PAGE followed by western blot with 10224-1-AP(UBC9 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human prostate cancer using 51018-2-AP(UBC9 antibody) at dilution of 1:100 (under 10x lens)
Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 51018-2-AP(UBC9 antibody) at dilution of 1:500
IP result of anti-UBE2I (51018-2-AP for IP and Detection) with mouse heart tissue lysate.
GTX41193 (0.3µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
GTX41193 (0.1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry: UBE2I Antibody [NB300-812] - NB300-812 (0.3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
NB 300-812 (0.1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
UBE2I polyclonal antibody ( Cat # PAB6303 ) staining ( 0.3 µg/ml ) of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
UBE2I polyclonal antibody ( Cat # PAB6303 ) staining ( 0.1 µg/ml ) of human kidney lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Human Jurkat; WB Suggested Anti-UBE2I Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate. There is BioGPS gene expression data showing that UBE2I is expressed in Jurkat.; UBE2I antibody - N-terminal region (ARP43021_T100) in Human Jurkat cells using Western Blot
Human Jurkat; WB Suggested Anti-UBE2I Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate. There is BioGPS gene expression data showing that UBE2I is expressed in Jurkat.; UBE2I antibody - middle region (ARP43022_T100) in Human Jurkat cells using Western Blot
Figure 1. (LEFT) Western blot analysis of anti-UBC9 Antibody (Cat. #AM11046PU-N) in Ramos cell line lysates (35ug/lane). UBC9(arrow) was detected using the purified Mab. (RIGHT) Western blot analysis of anti-UBC9 Antibody (C-term) (Cat. #AM11046PU-N) in mouse spleen tissue lysates (35ug/lane). UBC9(arrow) was detected using the purified Mab.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-UBE2I Antibody (N-term) (Cat.#AP11077PU-N) in A2058 cell line lysates (35ug/lane). UBE2I(arrow) was detected using the purified Pab.
AP16096PU-N (0.3µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
AP16096PU-N (0.1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: UBE2I Lysate [NBL1-17532] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for UBE2I
Western Blot: UBE2I Lysate [NBL1-17533] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for UBE2I.
Immunohistochemical of paraffin-embedded human lung cancer using 14837-1-AP(UBE2I-Specific antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using 14837-1-AP(UBE2I-Specific antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using 14837-1-AP( UBE2I-Specific Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
Human testis tissue were subjected to SDS PAGE followed by western blot with 14837-1-AP(UBE2I-Specific antibody) at dilution of 1:400
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14837-1-AP(UBE2I-Specific antibody) at dilution of 1:400
1X10^6 HepG2 cells were stained with 0.2ug UBE2I-Specific antibody (14837-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
Immunohistochemistry: UBE2I Antibody (EP2938Y) [NBP1-40517] - Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-UBC9
Western Blot: UBE2I Antibody (EP2938Y) [NBP1-40517] - Western blot analysis on (A) U937, (B) HeLa, (C) Jurkat and (D) HUVEC cell lysates using anti-UBC9, dilution 1:10,000.
Immunohistochemistry-Paraffin: UBE2I Antibody [NBP1-86887] - Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Western Blot: UBE2I Antibody [NBP1-86887] Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
Immunohistochemical analysis of paraffin-embedded prostate tissues using UBE2I mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using UBE2I mouse mAb with DAB staining.
Immunofluorescence analysis of HepG2 cells using UBE2I mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot analysis using UBE2I mAb against human UBE2I (AA: 1-158) recombinant protein. (Expected MW is 45.3 kDa)
Western blot analysis using UBE2I mouse mAb against Hela (1), HepG2 (2), and Cos7 (3) cell lysate.
Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)
Flow cytometric analysis of HepG2 cells using UBE2I mouse mAb (green) and negative control (red).
HEK-293 cells were subjected to SDS PAGE followed by western blot with 60201-1-Ig(UBC9 antibody) at dilution of 1:1000
Antigen: bs-8347P, 2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; TMB stainingRead the data in Microplate Reader by 450nm.
Western blot analysis of UBC9 expression in HeLa (A); A431 (B); Raw264.7 (C); PC12 (D) whole cell lysates.
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA327329 | UBC9 / UBE2I antibody |
||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€325.00
|
||
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
||||||
| AM06691SU-N | UBC9 / UBE2I antibody |
||||||
 |
Mouse | IgG1 | 1B10 | Ascites | Hu, Mky | E, F, ICC/IF, P, WB |
0.1 ml /
€430.00
|
| Acris Antibodies GmbH | |||||||
| +6 additional images |
Highly validated antibody! |
||||||
| AP16096PU-T | UBC9 / UBE2I (N-term) antibody |
||||||
 |
Goat | Aff - Purified | Can, Hu, Ms, Rt | E, P, WB |
20 µg /
€200.00
|
||
| Acris Antibodies GmbH | |||||||
Acris recommends this product for Western blotting. |
|||||||
| TA302696 | UBC9 / UBE2I (N-term) antibody |
||||||
|
Goat | Aff - Purified | Can, Hu, Ms, Rt | E, ICC/IF, P, WB |
0.1 mg /
€325.00
|
||
| OriGene Technologies, Inc. | |||||||
| AM11046PU-N | UBC9 / UBE2I antibody |
||||||
 |
Mouse | IgG1 | 67AT1273.95.90 | Purified | Hu, Ms | WB |
0.4 ml /
€370.00
|
| Acris Antibodies GmbH | |||||||
| AP11077PU-N | UBC9 / UBE2I (N-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€370.00
|
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
|||||||
| PAB2231 | UBC9 / UBE2I antibody |
||||||
 |
Rabbit | Hu | C, E, WB |
0.4 ml /
€390.00
|
|||
| Abnova Taiwan Corp. | |||||||
| PAB6303 | UBC9 / UBE2I antibody |
||||||
 |
Goat | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€400.00
|
|||
| Abnova Taiwan Corp. | |||||||
| +1 additional image |
|||||||
| ARP43021_T100 | UBC9 / UBE2I antibody |
||||||
 |
Rabbit | Aff - Purified | African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze | WB |
0.1 mg /
€310.00
|
||
| AVIVA Systems Biology | |||||||
| ARP43022_T100 | UBC9 / UBE2I antibody |
||||||
 |
Rabbit | Aff - Purified | African clawed frog, Chk, Hu, Ms, Rt | WB |
0.1 mg /
€310.00
|
||
| AVIVA Systems Biology | |||||||
| CPA2205-30ul | UBC9 / UBE2I antibody |
||||||
 |
Rabbit | Aff - Purified | Chk, Hu, Ms, Rt, Ze | WB |
30 µl /
€190.00
|
||
| Cohesion Biosciences Ltd. | |||||||
| CPA2205-100ul | UBC9 / UBE2I antibody |
||||||
|
Rabbit | Aff - Purified | Chk, Hu, Ms, Rt, Ze | WB |
0.1 ml /
€270.00
|
||
| Cohesion Biosciences Ltd. | |||||||
| CPA2205-200ul | UBC9 / UBE2I antibody |
||||||
|
Rabbit | Aff - Purified | Chk, Hu, Ms, Rt, Ze | WB |
0.2 ml /
€440.00
|
||
| Cohesion Biosciences Ltd. | |||||||
| NB300-812 | UBC9 / UBE2I antibody |
||||||
![Immunohistochemistry: UBE2I Antibody [NB300-812] - NB300-812 (0.3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/p_20097_2.jpg) |
Goat | IgG | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
0.1 mg /
€400.00
|
|
| Novus Biologicals Inc. | |||||||
| +1 additional image |
|||||||
| NBP1-40517 | UBC9 / UBE2I antibody |
||||||
![Immunohistochemistry: UBE2I Antibody (EP2938Y) [NBP1-40517] - Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-UBC9](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/117273_2.jpg) |
Rabbit | IgG | EP2938Y | Hu, Ms, Rt | F, ICC/IF, IP, P, WB |
0.1 ml /
€490.00
|
|
| Novus Biologicals Inc. | |||||||
| +1 additional image |
|||||||
| NBP1-86887 | UBC9 / UBE2I antibody |
||||||
![Immunohistochemistry-Paraffin: UBE2I Antibody [NBP1-86887] - Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/UBE2I Antibody-Immunohistochemistry-Paraffin-NBP1-86887-0005.jpg) |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 ml /
€500.00
|
|
| Novus Biologicals Inc. | |||||||
| +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301199 | UBC9 / UBE2I |
||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
| TP317884 | UBC9 / UBE2I |
||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
| TP317945 | UBC9 / UBE2I |
||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
Regular Price: 20 µg / €680.00 Special Price: 20 µg / €398.00 |
|
| OriGene Technologies, Inc. | |||||
|
|||||
| TP324182 | UBC9 / UBE2I |
||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
