UBC9 / UBE2I antibody

Principal name

UBC9 / UBE2I antibody

Alternative names for UBC9 / UBE2I antibody

UBCE9, Ube2i, Ubce2i, SUMO-protein ligase, SUMO-conjugating enzyme UBC9, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, Ubiquitin carrier protein I, Ubiquitin carrier protein 9

SwissProt ID

P63279 (Human), P63280 (Mouse), P63281 (Rat), P63282 (Xenla), P63283 (Chick)

Gene ID

7329 (UBE2I), 22196 (Ube2i), 25573

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Mky (Monkey)

Available hosts

Rabbit, Goat, Mouse

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of UBC9 / UBE2I antibody

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2I is a member of the E2 ubiquitin-conjugating enzyme family.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

General readings

1. Wang ZY, Qiu QQ, Seufert W, Taguchi T, Testa JR, Whitmore SA, Callen DF, Welsh D, Shenk T, Deuel TF. Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9. J Biol Chem. 1996 Oct 4;271(40):24811-6. PMID: 8798754.
2. Subramaniam S, Mealer RG, Sixt KM, Barrow RK, Usiello A, Snyder SH. Rhes, a physiologic regulator of sumoylation, enhances cross-sumoylation between the basic sumoylation enzymes E1 and Ubc9. J Biol Chem. 2010 Jul 2;285(27):20428-32. PMID: 20424159 (in WB on Mouse Brain).
3. Ihara M, Stein P, Schultz RM. UBE2I (UBC9), a SUMO-conjugating enzyme, localizes to nuclear speckles and stimulates transcription in mouse oocytes.
Biol Reprod. 2008 Nov;79(5):906-13. PMID: 18703419.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UBC9 / UBE2I antibody

  UBE2I Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using UBE2I antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

UBC9 / UBE2I antibody

Immunohistochemical analysis of paraffin-embedded prostate tissues using UBE2I mouse mAb with DAB staining. Mouse IgG1 1B10 Ascites Hu, Mky E, F, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

UBC9 / UBE2I (N-term) antibody

  Goat Polyclonal Antibody against UBE2I  
TA302696 (0.1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

UBC9 / UBE2I antibody

Figure 1. (LEFT) Western blot analysis of anti-UBC9 Antibody (Cat. #AM11046PU-N) in Ramos cell line lysates (35ug/lane). UBC9(arrow) was detected using the purified Mab. (RIGHT) Western blot analysis of anti-UBC9 Antibody (C-term) (Cat. #AM11046PU-N) in mouse spleen tissue lysates (35ug/lane). UBC9(arrow) was detected using the purified Mab. Mouse IgG1 67AT1273.95.90 Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

UBC9 / UBE2I (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

UBC9 / UBE2I antibody

UBC9 / UBE2I Rabbit Hu C, E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

UBC9 / UBE2I antibody

UBE2I polyclonal antibody ( Cat # PAB6303 ) staining ( 0.3 µg/ml ) of paraffin embedded human kidney.  Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Goat Hu, Ms, Rt E, P, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

UBC9 / UBE2I antibody

Human Jurkat; WB Suggested Anti-UBE2I Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate. There is BioGPS gene expression data showing that UBE2I is expressed in Jurkat.; UBE2I antibody - N-terminal region (ARP43021_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

UBC9 / UBE2I antibody

Human Jurkat; WB Suggested Anti-UBE2I Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate. There is BioGPS gene expression data showing that UBE2I is expressed in Jurkat.; UBE2I antibody - middle region (ARP43022_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Chk, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

UBC9 / UBE2I antibody

Western blot analysis of UBC9 expression in HeLa (A); A431 (B); Raw264.7 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Chk, Hu, Ms, Rt, Ze WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

UBC9 / UBE2I antibody

Western blot analysis of UBC9 expression in HeLa (A); A431 (B); Raw264.7 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Chk, Hu, Ms, Rt, Ze WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

UBC9 / UBE2I antibody

Western blot analysis of UBC9 expression in HeLa (A); A431 (B); Raw264.7 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Chk, Hu, Ms, Rt, Ze WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CRISPR

Catalog No. Species Donor Vector  

UBE2I - human gene knockout kit via CRISPR

UBE2I - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

UBE2I - human gene knockout kit via CRISPR

UBE2I - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

UBE2I - human gene knockout kit via CRISPR

UBE2I - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

UBE2I - human gene knockout kit via CRISPR

UBE2I - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ube2i - mouse gene knockout kit via CRISPR

Ube2i - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

UBC9 / UBE2I (transcript variant 4)

  Recombinant protein of human ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) (UBE2I), transcript variant 4  
UBC9 / UBE2I Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

UBC9 / UBE2I (transcript variant 1)

  Recombinant protein of human ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) (UBE2I), transcript variant 1  
UBC9 / UBE2I Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

UBC9 / UBE2I (transcript variant 2)

  Recombinant protein of human ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) (UBE2I), transcript variant 2  
UBC9 / UBE2I Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

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