UBE1 / UBA1 antibody

Principal name

UBE1 / UBA1 antibody

Alternative names for UBE1 / UBA1 antibody

Ubiquitin-activating enzyme E1, Protein A1S9, A1S9T, Ubiquitin-like modifier-activating enzyme 1

SwissProt ID

A3KMV5 (Bovin), P22314 (Human), Q02053 (Mouse), Q29504 (Rabit), Q5U300 (Rat)

Gene ID

7317 (UBA1), 3687317 (accA)

Available reactivities

Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Can (Canine), Bov (Bovine), Eq (Equine), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C)

Background of UBE1 / UBA1 antibody

Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitinprotein isopeptide bond formation. E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a highenergy thioester bond in an ATP-dependent manner. UBE1 is monomeric and contains two active sites within the E1 molecule, allowing it to bind two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR. Isoform 1 has a different 5' noncoding exon compared to isoform 2. Both variants encode the same protein.

General readings

1. Salvat C, Acquaviva C, Scheffner M, Robbins I, Piechaczyk M, Jariel-Encontre I, Molecular characterization of the thermosensitive E1 ubiquitin-activating enzyme cell mutant A31Nts20.
Requirements upon different levels of E1 for the ubiquitination/degradation of the various protein substrates in vivo. Eur J Biochem (2000) Jun;267(12):3712-22

17 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UBE1 / UBA1 antibody

UBE1 / UBA1 Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UBE1 / UBA1 antibody

WB using anti-Ubiquitin Activating Enzyme (E1) antibody shows detection of a band at ~118 kDa corresponding to UBE1 (lane 1800 nm channel). Primary antibody was used at a 1:1,000 dilution. Secondry antibody was a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L]. Molecular weight markers are shown in lane 2 (700 nm channel). Rabbit IgG Purified Can, Hu, Ms, Rb, Rt E, P, WB
0.5 mg / €325.00
  OriGene Technologies, Inc.

UBE1 / UBA1 antibody

WB Suggested Anti-UBA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

UBE1 / UBA1 (C-term) antibody

(LEFT) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (RIGHT) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with UBE1 antibody (C-term) (Cat.#AP11969PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

UBE1 / UBA1 antibody

UBE1 / UBA1 Mouse IgG2a 2B6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

UBE1 / UBA1 antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with UBA1 polyclonal antibody ( Cat # PAB1772 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

UBE1 / UBA1 antibody

Immunohistochemistry of UBA1 polyclonal antibody ( Cat # PAB11287 ) was used at a 10 µg/ml to detect UBA1 in a variety of tissues including adrenal, breast, colon ( epithelium ), kidney, liver, lung ( respiratory epithelium ), ovary ( oocyte and endothelium ), pancreas ( isletand exocrine ), placenta, prostate ( epithelium ), skin( epithelium ), spleen ( lymphocytes ), stomach ( chief ), testis,thymus, tonsil, and uterus ( glandular, stroma ).
In many cells a punctate nuclear staining was observed.
Other cells showedboth cytoplasmic and nuclear staining.
This image shows UBA1 staining of human lung tissue.
Tissue was formalin-fixed and paraffin embedded.
Personal Communi-cation, TinaRoush, Life Span Biosciences, Seattle, WA. Rabbit Purified Hu, Ms, Rb, Rt E, P, WB
0.5 mg / €560.00
  Abnova Taiwan Corp.

UBE1 / UBA1 antibody

UBE1 / UBA1 Rabbit Purified Hu C, E, WB
0.5 mg / €820.00
  Abnova Taiwan Corp.

UBE1 / UBA1 antibody

Human HepG2; WB Suggested Anti-UBA1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; UBA1 antibody - N-terminal region (ARP58687_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

UBE1 / UBA1 antibody

WB and IP: UBE1 Antibody [NB100-58804] - UBE1 Antibody [NB100-58804] - Detection of Human and Mouse UBE1 on HeLa whole cell lysate using NB100-58804. UBE1 was also IPed efficiently by rabbit anti-UBE1 antibodies NB100-58805 and NB100-58806. Rabbit Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

UBE1 / UBA1 antibody

Immunohistochemistry: UBE1 Antibody [NB600-472] - Immunohistochemistry. Affinity Purified anti-Ubiquitin Activating Enzyme antibody was used at a 10 ìg/ml to detect UBE1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (islet and exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis, thymus, tonsil, and uterus (glandular, stroma). In many cells a punctate nuclear staining was observed. Other cells showed both cytoplasmic and nuclear staining. This image shows UBE1 staining of human lung tissue. Tissue was formalinfixed and paraffin embedded. Rabbit IgG Hu E, P, WB
0.25 mg / €320.00
  Novus Biologicals Inc.

UBE1 / UBA1 antibody

Immunohistochemistry-Paraffin: E1 Ubiquitin Activating Enzyme Antibody [NBP1-90307] - Immunohistochemical staining of human cerebral cortex shows distinct nuclear positivity in glial cells. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

UBE1 / UBA1 (transcript variant 1)

UBE1 / UBA1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

UBE1 / UBA1

UBE1 / UBA1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

UBE1 / UBA1

UBE1 / UBA1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

UBE1 / UBA1

UBE1 / UBA1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

UBE1 / UBA1

UBE1 / UBA1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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