UBE2B / RAD6B antibody
Principal name
UBE2B / RAD6B antibody
Alternative names for UBE2B / RAD6B antibody
Ubiquitin-conjugating enzyme E2 B, Ubiquitin-protein ligase B, Ubiquitin carrier protein B, HR6B, hHR6B, E2-17 kDa
Gene ID
7320 (UBE2B)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of UBE2B / RAD6B antibody
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
Xin H, et al. (2000) Nucleic Acids Res.. 28(14):2847-54.
Immunohistochemical of paraffin-embedded human ovary tumor using 10733-1-AP(UBE2B antibody) at dilution of 1:10 (under 10x lens)
L02 cells were subjected to SDS PAGE followed by western blot with 10733-1-AP( UBE2B Antibody) at dilution of 1:600
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 10733-1-AP( UBE2B Antibody) at dilution of 1:1000
Western Blot analysis of UBE2B expression in transfected 293T cell line (H00007320-T01) by UBE2B MaxPab polyclonal antibody.Lane 1: UBE2B transfected lysate(16.72 KDa).Lane 2: Non-transfected lysate.
UBE2B MaxPab polyclonal antibody. Western Blot analysis of UBE2B expression in Jurkat.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBE2B polyclonal antibody ( Cat # PAB1776 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
The UBE2B polyclonal antibody ( Cat # PAB1776 ) is used in Western blot to detect UBE2B in A-375 cell lysate.
Western Blot analysis of UBE2B expression in transfected 293T cell line by UBE2B monoclonal antibody (M06), clone 4C3.Lane 1: UBE2B transfected lysate(17.3 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged UBE2B is approximately 3ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western Blot analysis using anti-UBE2B (C-term) Pab (Cat.#AP11973PU-N) to detect UBE2B in A375 cell lysate.
Western Blot: Ube2B Lysate [NBL1-17513] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for UBE2B
Immunofluorescence analysis of paraformaldehyde-fixed A431, using UBE2B(GTX100416) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE GTX100416 diluted at 1:1000
UBE2B antibody detects UBE2B protein by western blot analysis.A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of 3xFlag-human UBE2B-transfected 293T cells12 % SDS-PAGEUBE2B antibody (GTX100416) dilution: 1:5000
Western Blot: UBE2B Antibody [NBP1-31293] - Western blot analysis of UBE2B Antibody on: A: Hela 12% SDS PAGE diluted at 1:1000
Western Blot analysis of UBE2B expression in transfected 293T cell line (H00007320-T01) by UBE2B MaxPab polyclonal antibody.Lane 1: UBE2B transfected lysate(16.72 KDa).Lane 2: Non-transfected lysate.
UBE2B MaxPab polyclonal antibody. Western Blot analysis of UBE2B expression in Jurkat.
Recombinant human UBE2B, 1-152 aa, His-tagged: 15% SDS-PAGE (3 ug)
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE GTX114867 diluted at 1:1000
SDS-Page: Ube2B Protein [NBP1-44455] - UBE2B, 21.4 kDa (188aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2B (RC203306, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2B.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308525 | UBE2B / RAD6B antibody |
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Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, WB |
0.1 ml /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA803573 | UBE2B / RAD6B antibody |
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 |
Mouse | IgG2b | OTI1E11 | Purified | Hu | WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
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| AP11973PU-N | UBE2B / RAD6B (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€370.00
|
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| H00007320-A01 | UBE2B / RAD6B antibody |
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 |
Mouse | Serum | Hu | E, WB |
50 µl /
€290.00
|
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| Abnova Taiwan Corp. | |||||||
| H00007320-B01 | UBE2B / RAD6B antibody |
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 |
Mouse | Serum | Hu | E(detection), WB |
50 µl /
€370.00
|
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| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| H00007320-B01P | UBE2B / RAD6B antibody |
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 |
Mouse | Purified | Hu | E(detection), WB |
50 µg /
€370.00
|
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| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| H00007320-M06 | UBE2B / RAD6B antibody |
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 |
Mouse | IgG2a | 4C3 | Purified | Hu | E, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| H00007320-M13 | UBE2B / RAD6B antibody |
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|
Mouse | IgG2a | 1F11 | Purified | Hu | E, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| PAB1776 | UBE2B / RAD6B antibody |
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 |
Rabbit | Hu | E, P, WB |
0.1 mg /
€390.00
|
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| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| NBP1-31293 | UBE2B / RAD6B antibody |
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![Western Blot: UBE2B Antibody [NBP1-31293] - Western blot analysis of UBE2B Antibody on: A: Hela 12% SDS PAGE diluted at 1:1000](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/109743_1.jpg) |
Rabbit | IgG | Hu | E, ICC/IF, WB |
0.1 ml /
€420.00
|
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| Novus Biologicals Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303306 | UBE2B / RAD6B |
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
| TP720719 | UBE2B / RAD6B |
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Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
10 µg /
€139.00
|
|
| OriGene Technologies, Inc. | |||||
| AR09479PU-L | UBE2B / RAD6B (1-152, His-tag) |
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 |
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.25 mg /
€750.00
|
| Acris Antibodies GmbH | |||||
| +1 additional image |
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| AR09479PU-N | UBE2B / RAD6B (1-152, His-tag) |
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Human | Purified | > 90 % by SDS - PAGE | E. coli |
50 µg /
€300.00
|
| Acris Antibodies GmbH | |||||
| +1 additional image |
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| H00007320-P01-10 | UBE2B / RAD6B |
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Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
10 µg /
€380.00
|
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| Abnova Taiwan Corp. | |||||
| H00007320-P01-25 | UBE2B / RAD6B |
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Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
25 µg /
€510.00
|
|
| Abnova Taiwan Corp. | |||||
| H00007320-Q01-10 | UBE2B / RAD6B |
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Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
10 µg /
€380.00
|
|
| Abnova Taiwan Corp. | |||||
| H00007320-Q01-25 | UBE2B / RAD6B |
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|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
25 µg /
€510.00
|
|
| Abnova Taiwan Corp. | |||||
| NBP1-44455 | UBE2B / RAD6B |
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![SDS-Page: Ube2B Protein [NBP1-44455] - UBE2B, 21.4 kDa (188aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Ube2B Protein-SDS-Page-NBP1-44455-img0001.jpg) |
Human | Purified |
50 µg /
€380.00
|
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| Novus Biologicals Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| H00007320-T01 | UBE2B 293T Cell Transient Overexpression Lysate(Denatured) |
|
|
0.1 ml /
€270.00
|
|
| Abnova Taiwan Corp. |
