UBE2B / RAD6B antibody

Principal name

UBE2B / RAD6B antibody

Alternative names for UBE2B / RAD6B antibody

Ubiquitin-conjugating enzyme E2 B, Ubiquitin-protein ligase B, Ubiquitin carrier protein B, HR6B, hHR6B, E2-17 kDa

SwissProt ID

P63146 (Human), P63147 (Mouse), P63148 (Rabit), P63149 (Rat), Q32P99 (Bovin)

Gene ID

7320 (UBE2B)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of UBE2B / RAD6B antibody

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Xin H, et al. (2000) Nucleic Acids Res.. 28(14):2847-54.

20 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UBE2B / RAD6B antibody

UBE2B antibody detects UBE2B protein by western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of 3xFlag-human UBE2B-transfected 293T cells. 12 % SDS-PAGE. UBE2B antibody (TA308525) dilution: 1:5000 Rabbit IgG Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UBE2B / RAD6B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2B (RC203306, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2B. Mouse IgG2b OTI1E11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

UBE2B / RAD6B (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

UBE2B / RAD6B antibody

UBE2B / RAD6B Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

UBE2B / RAD6B antibody

Western Blot analysis of UBE2B expression in transfected 293T cell line (H00007320-T01) by UBE2B MaxPab polyclonal antibody.

Lane 1: UBE2B transfected lysate(16.72 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

UBE2B / RAD6B antibody

Western Blot analysis of UBE2B expression in transfected 293T cell line (H00007320-T01) by UBE2B MaxPab polyclonal antibody.

Lane 1: UBE2B transfected lysate(16.72 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

UBE2B / RAD6B antibody

Western Blot analysis of UBE2B expression in transfected 293T cell line by UBE2B monoclonal antibody (M06), clone 4C3.

Lane 1: UBE2B transfected lysate(17.3 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 4C3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

UBE2B / RAD6B antibody

UBE2B / RAD6B Mouse IgG2a 1F11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

UBE2B / RAD6B antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBE2B polyclonal antibody ( Cat # PAB1776 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

UBE2B / RAD6B antibody

Western Blot: UBE2B Antibody [NBP1-31293] - Western blot analysis of UBE2B Antibody on: A: Hela 12% SDS PAGE diluted at 1:1000 Rabbit IgG Hu E, ICC/IF, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

UBE2B / RAD6B

UBE2B / RAD6B Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

UBE2B / RAD6B

UBE2B / RAD6B Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €139.00
  OriGene Technologies, Inc.

UBE2B / RAD6B (1-152, His-tag)

UBE2B / RAD6B Human Purified > 90 % by SDS - PAGE E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

UBE2B / RAD6B (1-152, His-tag)

UBE2B / RAD6B Human Purified > 90 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

UBE2B / RAD6B

UBE2B / RAD6B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

UBE2B / RAD6B

UBE2B / RAD6B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

UBE2B / RAD6B

UBE2B / RAD6B Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

UBE2B / RAD6B

UBE2B / RAD6B Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

UBE2B / RAD6B

SDS-Page: Ube2B Protein [NBP1-44455] - UBE2B, 21.4 kDa (188aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE Human Purified
50 µg / €380.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

UBE2B 293T Cell Transient Overexpression Lysate(Denatured)

UBE2B 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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