UBE2B / RAD6B antibody

Principal name

UBE2B / RAD6B antibody

Alternative names for UBE2B / RAD6B antibody

Ubiquitin-conjugating enzyme E2 B, Ubiquitin-protein ligase B, Ubiquitin carrier protein B, HR6B, hHR6B, E2-17 kDa

SwissProt ID

P63146 (Human), P63147 (Mouse), P63148 (Rabit), P63149 (Rat), Q32P99 (Bovin)

Gene ID

7320 (UBE2B), 22210 (Ube2b), 81816

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of UBE2B / RAD6B antibody

Kit Component:
- KN318563G1, Ube2b gRNA vector 1 in pCas-Guide vector
- KN318563G2, Ube2b gRNA vector 2 in pCas-Guide vector
- KN318563D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Xin H, et al. (2000) Nucleic Acids Res.. 28(14):2847-54.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UBE2B / RAD6B antibody

  Rabbit Polyclonal antibody to UBE2B (ubiquitin-conjugating enzyme E2B (RAD6 homolog))  
UBE2B antibody detects UBE2B protein by western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of 3xFlag-human UBE2B-transfected 293T cells. 12 % SDS-PAGE. UBE2B antibody (TA308525) dilution: 1:5000 Rabbit IgG Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UBE2B / RAD6B antibody

  UBE2B mouse monoclonal antibody, clone OTI1E11 (formerly 1E11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2B (RC203306, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2B. Mouse IgG2b OTI1E11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

UBE2B / RAD6B (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

UBE2B - human gene knockout kit via CRISPR

UBE2B - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

UBE2B - human gene knockout kit via CRISPR

UBE2B - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

UBE2B - human gene knockout kit via CRISPR

UBE2B - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

UBE2B - human gene knockout kit via CRISPR

UBE2B - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ube2b - mouse gene knockout kit via CRISPR

Ube2b - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ube2b - mouse gene knockout kit via CRISPR

Ube2b - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ube2b - mouse gene knockout kit via CRISPR

Ube2b - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ube2b - mouse gene knockout kit via CRISPR

Ube2b - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

UBE2B / RAD6B

  Recombinant protein of human ubiquitin-conjugating enzyme E2B (RAD6 homolog) (UBE2B)  
UBE2B / RAD6B Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

UBE2B / RAD6B

  Purified recombinant protein of Human ubiquitin-conjugating enzyme E2B (UBE2B)  
UBE2B / RAD6B Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €139.00
  OriGene Technologies, Inc.

UBE2B / RAD6B (1-152, His-tag)

UBE2B / RAD6B Human Purified > 90 % by SDS - PAGE E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

UBE2B / RAD6B (1-152, His-tag)

UBE2B / RAD6B Human Purified > 90 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, UBE2B (mGFP-tagged) - Human ubiquitin-conjugating enzyme E2B (UBE2B), 200 uL, >10^7 TU/mL

Lenti ORF particles, UBE2B (mGFP-tagged) - Human ubiquitin-conjugating enzyme E2B (UBE2B), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, UBE2B (Myc-DDK tagged) - Human ubiquitin-conjugating enzyme E2B (UBE2B) , 200 uL, >10^7 TU/mL

Lenti ORF particles, UBE2B (Myc-DDK tagged) - Human ubiquitin-conjugating enzyme E2B (UBE2B) , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Ube2b (GFP-tagged) - Mouse ubiquitin-conjugating enzyme E2B, RAD6 homology (S. cerevisiae) (Ube2b), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ube2b (GFP-tagged) - Mouse ubiquitin-conjugating enzyme E2B, RAD6 homology (S. cerevisiae) (Ube2b), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Ube2b (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2B, RAD6 homology (S. cerevisiae) (Ube2b), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ube2b (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2B, RAD6 homology (S. cerevisiae) (Ube2b), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Ube2b (GFP-tagged ORF) - Rat ubiquitin-conjugating enzyme E2B, RAD6 homology (S. cerevisiae) (Ube2b), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ube2b (GFP-tagged ORF) - Rat ubiquitin-conjugating enzyme E2B, RAD6 homology (S. cerevisiae) (Ube2b), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

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