UBE2B / RAD6B antibody
Principal name
UBE2B / RAD6B antibody
Alternative names for UBE2B / RAD6B antibody
Ubiquitin-conjugating enzyme E2 B, Ubiquitin-protein ligase B, Ubiquitin carrier protein B, HR6B, hHR6B, E2-17 kDa
Ncbi ID
9606, 5874, NP_003328, NP_003328.1, NP_033484.3, NM_009458, NM_031138, NM_003337
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of UBE2B / RAD6B antibody
- Kit Component:
- - KN318563G1, Ube2b gRNA vector 1 in pCas-Guide vector
- KN318563G2, Ube2b gRNA vector 2 in pCas-Guide vector
- KN318563D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Xin H, et al. (2000) Nucleic Acids Res.. 28(14):2847-54.
Western Blot analysis of UBE2B expression in transfected 293T cell line (H00007320-T01) by UBE2B MaxPab polyclonal antibody.Lane 1: UBE2B transfected lysate(16.72 KDa).Lane 2: Non-transfected lysate.
UBE2B MaxPab polyclonal antibody. Western Blot analysis of UBE2B expression in Jurkat.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBE2B polyclonal antibody ( Cat # PAB1776 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
The UBE2B polyclonal antibody ( Cat # PAB1776 ) is used in Western blot to detect UBE2B in A-375 cell lysate.
Western Blot analysis of UBE2B expression in transfected 293T cell line by UBE2B monoclonal antibody (M06), clone 4C3.Lane 1: UBE2B transfected lysate(17.3 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged UBE2B is approximately 3ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western Blot analysis using anti-UBE2B (C-term) Pab (Cat.#AP11973PU-N) to detect UBE2B in A375 cell lysate.
Western Blot analysis of UBE2B expression in transfected 293T cell line (H00007320-T01) by UBE2B MaxPab polyclonal antibody.Lane 1: UBE2B transfected lysate(16.72 KDa).Lane 2: Non-transfected lysate.
UBE2B MaxPab polyclonal antibody. Western Blot analysis of UBE2B expression in Jurkat.
Recombinant human UBE2B, 1-152 aa, His-tagged: 15% SDS-PAGE (3 ug)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2B (RC203306, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2B.
UBE2B antibody detects UBE2B protein by western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of 3xFlag-human UBE2B-transfected 293T cells. 12 % SDS-PAGE. UBE2B antibody (TA308525) dilution: 1:5000
Sample (30 ug of whole cell lysate). A: NIH-3T3 12% SDS PAGE. TA308525 diluted at 1:1000.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using UBE2B(TA308525) antibody at 1:500 dilution.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308525 | UBE2B / RAD6B antibody |
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| Rabbit Polyclonal antibody to UBE2B (ubiquitin-conjugating enzyme E2B (RAD6 homolog)) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, WB |
0.1 ml /
€416.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA803573 | UBE2B / RAD6B antibody |
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| UBE2B mouse monoclonal antibody, clone OTI1E11 (formerly 1E11) | |||||||
 |
Mouse | IgG2b | OTI1E11 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AP11973PU-N | UBE2B / RAD6B (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€370.00
|
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN203306 | UBE2B - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203306BN | UBE2B - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203306LP | UBE2B - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203306RB | UBE2B - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN318563 | Ube2b - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN318563BN | Ube2b - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN318563LP | Ube2b - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN318563RB | Ube2b - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303306 | UBE2B / RAD6B |
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| Recombinant protein of human ubiquitin-conjugating enzyme E2B (RAD6 homolog) (UBE2B) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
| TP720719 | UBE2B / RAD6B |
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| Purified recombinant protein of Human ubiquitin-conjugating enzyme E2B (UBE2B) | |||||
|
Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
10 µg /
€147.00
|
|
| OriGene Technologies, Inc. | |||||
| AR09479PU-L | UBE2B / RAD6B (1-152, His-tag) |
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 |
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.25 mg /
€750.00
|
| Acris Antibodies GmbH | |||||
| +1 additional image |
|||||
| AR09479PU-N | UBE2B / RAD6B (1-152, His-tag) |
||||
|
Human | Purified | > 90 % by SDS - PAGE | E. coli |
50 µg /
€300.00
|
| Acris Antibodies GmbH | |||||
| +1 additional image |
|||||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC203306L2V | Lenti ORF particles, UBE2B (mGFP-tagged) - Human ubiquitin-conjugating enzyme E2B (UBE2B), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€907.00
|
|
| OriGene Technologies, Inc. | ||
| RC203306L1V | Lenti ORF particles, UBE2B (Myc-DDK tagged) - Human ubiquitin-conjugating enzyme E2B (UBE2B) , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€804.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR226043L2V | Lenti ORF particles, Ube2b (GFP-tagged) - Mouse ubiquitin-conjugating enzyme E2B, RAD6 homology (S. cerevisiae) (Ube2b), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€907.00
|
|
| OriGene Technologies, Inc. | ||
| MR226043L1V | Lenti ORF particles, Ube2b (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2B, RAD6 homology (S. cerevisiae) (Ube2b), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€804.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR201391L2V | Lenti ORF particles, Ube2b (GFP-tagged ORF) - Rat ubiquitin-conjugating enzyme E2B, RAD6 homology (S. cerevisiae) (Ube2b), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€907.00
|
|
| OriGene Technologies, Inc. | ||
