UBE2C / UBCH10 antibody

Principal name

UBE2C / UBCH10 antibody

Alternative names for UBE2C / UBCH10 antibody

Ubiquitin-conjugating enzyme E2 C, Ubiquitin-protein ligase C, Ubiquitin carrier protein C

SwissProt ID

O00762 (Human), Q32PA5 (Bovin), Q9D1C1 (Mouse)

Gene ID

11065 (UBE2C)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)

Background of UBE2C / UBCH10 antibody

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2C is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human UBE2C with an internal ID of P25065

General readings

Townsley FM, Aristarkhov A, Beck S, Hershko A, Ruderman JV.
Dominant-negative cyclin-selective ubiquitin carrier protein E2-C/UbcH10 blocks cells in metaphase.
Proc Natl Acad Sci U S A 1997 Mar 18;94(6):2362-7
PMID: 9122200

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UBE2C / UBCH10 antibody

Immunoperoxidase of monoclonal antibody to UBE2C on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml] Mouse IgG2a 9D3 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

UBE2C / UBCH10 antibody

(1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

UBE2C / UBCH10 antibody

WB Suggested Anti-UBE2C Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Muscle Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

UBE2C / UBCH10 antibody

WB Suggested Anti-UBE2C Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Muscle Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

UBE2C / UBCH10 antibody

WB Suggested Anti-UBE2C Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

UBE2C / UBCH10 antibody

Western blot analysis of extracts of various cell lines, using UBE2C antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

UBE2C / UBCH10 (C-term) antibody

UBE2C  / UBCH10 Goat Aff - Purified Hu E, ICC/IF, IP, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

UBE2C / UBCH10 (C-term) antibody

UBE2C  / UBCH10 Goat Aff - Purified Hu E, ICC/IF, IP, WB
20 µg / €200.00
  Acris Antibodies GmbH

UBE2C / UBCH10 (N-term) antibody

Western blot analysis of UBE2C (arrow) using rabbit polyclonal UBE2C Antibody (N-term G25) (AP17818PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2C gene (Lane 2) (Origene Technologies).(8ug/ml) Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

UBE2C / UBCH10 antibody

UBE2C  / UBCH10 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

UBE2C / UBCH10 antibody

UBE2C polyclonal antibody (A02), Lot # 051122JC01 Western Blot analysis of UBE2C expression in IMR-32 ( Cat # L008V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

UBE2C / UBCH10 antibody

Western Blot analysis of UBE2C expression in transfected 293T cell line by UBE2C monoclonal antibody (M04), clone 3B1.

Lane 1: UBE2C transfected lysate(19.7 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 3B1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

UBE2C / UBCH10 antibody

UBE2C  / UBCH10 Mouse IgG2b 1D8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

UBE2C / UBCH10 antibody

UBE2C  / UBCH10 Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

UBE2C / UBCH10 antibody

Human Muscle; WB Suggested Anti-UBE2C Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Muscle; UBE2C antibody - N-terminal region (ARP43090_P050) in Human Muscle cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

UBE2C / UBCH10 antibody

Human Muscle; WB Suggested Anti-UBE2C Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Muscle; UBE2C antibody - middle region (ARP43091_P050) in Human Muscle cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

UBE2C / UBCH10 (Center) antibody

Human THP-1; WB Suggested Anti-UBE2C Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: THP-1 cell lysate; UBE2C antibody - middle region (ARP43474_P050) in Human THP-1 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

UBE2C / UBCH10 antibody

UBE2C  / UBCH10 Goat IgG Aff - Purified Hu, Ms, Rt E
0.1 mg / €400.00
  Novus Biologicals Inc.

UBE2C / UBCH10 antibody

Western Blot: UBE2C Antibody [NBP1-58165] - This Anti-UBE2C antibody was used in Western Blot of Fetal Muscle tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

UBE2C / UBCH10 antibody

Western Blot: UBE2C Antibody [NBP1-58166] - This Anti-UBE2C antibody was used in Western Blot of Fetal Muscle tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms WB
50 µg / €440.00
  Novus Biologicals Inc.

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