UBE2C / UBCH10 antibody

Principal name

UBE2C / UBCH10 antibody

Alternative names for UBE2C / UBCH10 antibody

Ubiquitin-conjugating enzyme E2 C, Ubiquitin-protein ligase C, Ubiquitin carrier protein C

SwissProt ID

O00762 (Human), Q32PA5 (Bovin), Q9D1C1 (Mouse)

Gene ID

11065 (UBE2C), 68612 (Ube2c)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of UBE2C / UBCH10 antibody

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2C is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human UBE2C with an internal ID of P25065

General readings

Townsley FM, Aristarkhov A, Beck S, Hershko A, Ruderman JV.
Dominant-negative cyclin-selective ubiquitin carrier protein E2-C/UbcH10 blocks cells in metaphase.
Proc Natl Acad Sci U S A 1997 Mar 18;94(6):2362-7
PMID: 9122200

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UBE2C / UBCH10 antibody

  Goat Anti-UBE2C / UBCH10 Antibody  
(1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

UBE2C / UBCH10 antibody

  Rabbit Polyclonal Anti-UBE2C Antibody  
WB Suggested Anti-UBE2C Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Muscle Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

UBE2C / UBCH10 antibody

  Rabbit Polyclonal Anti-UBE2C Antibody  
WB Suggested Anti-UBE2C Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Muscle Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

UBE2C / UBCH10 antibody

  Rabbit Polyclonal Anti-UBE2C Antibody  
WB Suggested Anti-UBE2C Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

UBE2C / UBCH10 antibody

  Rabbit anti-UBE2C Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using UBE2C antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

UBE2C / UBCH10 (C-term) antibody

UBE2C  / UBCH10 Goat Aff - Purified Hu E, ICC/IF, IP, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

UBE2C / UBCH10 (C-term) antibody

UBE2C  / UBCH10 Goat Aff - Purified Hu E, ICC/IF, IP, WB
20 µg / €200.00
  Acris Antibodies GmbH

UBE2C / UBCH10 (N-term) antibody

Western blot analysis of UBE2C (arrow) using rabbit polyclonal UBE2C Antibody (N-term G25) (AP17818PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2C gene (Lane 2) (Origene Technologies).(8ug/ml) Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

UBE2C - human gene knockout kit via CRISPR

UBE2C - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

UBE2C - human gene knockout kit via CRISPR

UBE2C - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

UBE2C - human gene knockout kit via CRISPR

UBE2C - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

UBE2C - human gene knockout kit via CRISPR

UBE2C - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ube2c - mouse gene knockout kit via CRISPR

Ube2c - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ube2c - mouse gene knockout kit via CRISPR

Ube2c - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ube2c - mouse gene knockout kit via CRISPR

Ube2c - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ube2c - mouse gene knockout kit via CRISPR

Ube2c - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

UBE2C / UBCH10 (transcript variant 4)

  Recombinant protein of human ubiquitin-conjugating enzyme E2C (UBE2C), transcript variant 4  
UBE2C  / UBCH10 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

UBE2C / UBCH10 (transcript variant 1)

  Recombinant protein of human ubiquitin-conjugating enzyme E2C (UBE2C), transcript variant 1  
UBE2C  / UBCH10 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

UBE2C / UBCH10 (1-179, His-tag)

UBE2C  / UBCH10 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

UBE2C / UBCH10 (1-179, His-tag)

UBE2C  / UBCH10 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

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