UBE2E2 antibody

Principal name

UBE2E2 antibody

Alternative names for UBE2E2 antibody

Ubiquitin-conjugating enzyme E2 E2, Ubiquitin-protein ligase E2, Ubiquitin carrier protein E2, UBCH8

SwissProt ID

Q91W82 (Mouse), Q96LR5 (Human)

Gene ID

7325 (UBE2E2), 218793 (Ube2e2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C)

Background of UBE2E2 antibody

Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). The first step in the ubiquitination process requires the ATP-dependent activation of the ubiquitin C-terminus and the assembly of multi-ubiquitin chains by the E1 enzyme. The ubiquitin chain is then conjugated to the E2 enzyme to generate an intermediate ubiquitin-E2 complex. The E3 enzyme then catalyzes the transfer of ubiquitin from E2 to the appropriate protein substrate, thereby targeting that substrate for degradation. A wide range of enzymes facilitate this proteolytic ubiquitin pathway, one of which is UBE2E2 (also known as UBCH8 in human), which functions as an E2 enzyme and catalyzes the ATP-dependent covalent attachment of ubiquitin to target proteins, thereby playing an important role in protein degradation.

General readings

Plafker,S.M., (2004) J. Cell Biol. 167 (4), 649-659

10 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UBE2E2 antibody

Host: Rabbit; Target Name: Ube2e2; Sample Tissue: Mouse Heart lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

UBE2E2 antibody

Lanes: 1: 40 ng HIS-UBE2D1 protein; 2: 40 ng HIS-UBE2D2 protein; 3: 40 ng HIS-UBE2D3 protein; 4: 40 ng HIS-UBE2D4 protein; 5: 40 ng HIS-UBE2E1 protein; 6: 40 ng HIS-UBE2E2 protein; 7: 40 ng HIS-UBE2E3 protein; 8: 40 ng HIS-UBE2K protein; 9: 40 ng HIS-UBE2 Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

UBE2E2 antibody

Immunofluorescence of monoclonal antibody to UBE2E2 on HeLa cell . [antibody concentration 10 ug/ml] Mouse IgG1 4B4 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

UBE2E2 antibody

Human kidney; UBE2E2 antibody - N-terminal region (ARP43437_P050) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Por, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

UBE2E2 antibody

Western Blot: UBE2E2 Antibody [NBP1-55077] - This Anti-UBE2E2 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 0.125ug/ml. Rabbit Aff - Purified Can, Hu, Ms, Rt P, WB
50 µg / €440.00
  Novus Biologicals Inc.

UBE2E2 antibody

UBE2E2 Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

UBE2E2 antibody

Western Blot: UBE2E2 Antibody [NBP1-80573]-Whole cell lysate from 293T (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-UBE2E2 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate.  UBE2E2 was also immunoprecipitated by rabbit anti-UBE2E2 antibody NBP1-80572, which recognizes an upstream epitope. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

UBE2E2 antibody

Immunohistochemistry-Paraffin: UBE2E2 Antibody [NBP1-92556] - Immunohistochemical staining of human cerebellum shows moderate cytoplasmic, nuclear and nuclear membrane positivity in purkinje cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

UBE2E2

UBE2E2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

UBE2E2

UBE2E2 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

10 Item(s)

per page
  • LinkedIn