UBE2F antibody

Principal name

UBE2F antibody

Alternative names for UBE2F antibody

NEDD8-conjugating enzyme 2, NEDD8 carrier protein, NEDD8 protein ligase UBE2F, Ubiquitin-conjugating enzyme E2 F

SwissProt ID

Q969M7 (Human), Q9CY34 (Mouse)

Gene ID

140739 (UBE2F), 363284

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), African clawed frog, Por (Porcine)

Available hosts

Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of UBE2F antibody

NCE2 is a ubiquitin conjugating enzyme. Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.

General readings

Huang D.T., et al. (2009) Mol. Cell 33:483-495Nandi, D. et al. (2006) Journal of biosciences 31 (1): 137-55.

16 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UBE2F (C-term) antibody

Staining of paraffin embedded Human Placenta using UBE2F Antibody Cat.-No AP16957PU-N at 2.5-3.8 µg/ml. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

UBE2F (N-term) antibody

(0.05ug/ml) staining of Rat Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

UBE2F antibody

Sample Type: 1. Human NT-2 cells (60ug); Primary Antibody Dilution: �2 ug/ml; Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience; Secondary Antibody Dilution: �1: 20, 000; Image Submitted by: Yuzhi Chen; University of Arkansas for Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt ICC/IF, WB
50 µg / €325.00
  OriGene Technologies, Inc.

UBE2F antibody

WB Suggested Anti-UBE2F Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 293T cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

UBE2F antibody

UBE2F Goat Hu E
0.1 mg / €390.00
  Abnova Taiwan Corp.

UBE2F antibody

Human Brain; Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical Science. Application: IHC. Species+tissue/cell type: Human brain stem cells. Primary antibody dilution: 1:500. Secondary antibody: Goat anti-rabbit Alexa-Fluor 594. Secondary antibody dilution: 1:1000; UBE2F antibody - N-terminal region (ARP52515_P050) in Human Brain cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

UBE2F antibody

UBE2F Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

UBE2F antibody

Immunohistochemistry-Paraffin: NCE2 Antibody [NBP1-44062] - In paraffin embedded Human Placenta shows membrane staining in trophoblasts Goat Aff - Purified Hu, Ms E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

UBE2F antibody

Western Blot: NCE2 Antibody [NBP1-57602] - This Anti-UBE2F antibody was used in Western Blot of Hela tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €440.00
  Novus Biologicals Inc.

UBE2F antibody

Immunohistochemistry-Paraffin: NCE2 Antibody [NBP1-92169] - Immunohistochemical staining of human cerebellum shows strong nucleolar positivity in Purkinje cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

UBE2F

UBE2F Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

UBE2F (1-185, His-tag)

UBE2F Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

UBE2F (1-185, His-tag)

UBE2F Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

UBE2F

UBE2F Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

UBE2F

UBE2F Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

UBE2F overexpression lysate

UBE2F overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

16 Item(s)

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