UBE2F antibody

Principal name

UBE2F antibody

Alternative names for UBE2F antibody

NEDD8-conjugating enzyme 2, NEDD8 carrier protein, NEDD8 protein ligase UBE2F, Ubiquitin-conjugating enzyme E2 F

SwissProt ID

Q1RMW1, Q5U203, Q969M7 (Human), Q9CY34 (Mouse)

Gene ID

140739 (UBE2F), 67921 (Ube2f), 363284

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), African clawed frog, Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ye (Yeast), Ze (Zebrafish)

Available hosts

Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of UBE2F antibody

NCE2 is a ubiquitin conjugating enzyme. Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.

General readings

Huang D.T., et al. (2009) Mol. Cell 33:483-495Nandi, D. et al. (2006) Journal of biosciences 31 (1): 137-55.

18 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UBE2F (C-term) antibody

Staining of paraffin embedded Human Placenta using UBE2F Antibody Cat.-No AP16957PU-N at 2.5-3.8 µg/ml. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

UBE2F (N-term) antibody

  Goat Anti-UBE2F / NCE2 (N Terminus) Antibody  
(0.05ug/ml) staining of Rat Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

UBE2F antibody

  Rabbit Polyclonal Anti-UBE2F Antibody  
Sample Type: 1. Human NT-2 cells (60ug); Primary Antibody Dilution: �2 ug/ml; Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience; Secondary Antibody Dilution: �1: 20, 000; Image Submitted by: Yuzhi Chen; University of Arkansas for Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt ICC/IF, WB
50 µg / €325.00
  OriGene Technologies, Inc.

UBE2F antibody

  Rabbit Polyclonal Anti-UBE2F Antibody  
WB Suggested Anti-UBE2F Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 293T cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

UBE2F - human gene knockout kit via CRISPR

UBE2F - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

UBE2F - human gene knockout kit via CRISPR

UBE2F - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

UBE2F - human gene knockout kit via CRISPR

UBE2F - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

UBE2F - human gene knockout kit via CRISPR

UBE2F - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

UBE2F

  Recombinant protein of human ubiquitin-conjugating enzyme E2F (putative) (UBE2F)  
UBE2F Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

UBE2F (1-185, His-tag)

UBE2F Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

UBE2F (1-185, His-tag)

UBE2F Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

UBE2F overexpression lysate

UBE2F overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, UBE2F (mGFP-tagged) - Human ubiquitin-conjugating enzyme E2F (putative) (UBE2F), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, UBE2F (mGFP-tagged) - Human ubiquitin-conjugating enzyme E2F (putative) (UBE2F), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, UBE2F (Myc-DDK tagged) - Human ubiquitin-conjugating enzyme E2F (putative) (UBE2F), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, UBE2F (Myc-DDK tagged) - Human ubiquitin-conjugating enzyme E2F (putative) (UBE2F), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Ube2f (GFP-tagged) - Mouse ubiquitin-conjugating enzyme E2F (putative) (Ube2f), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ube2f (GFP-tagged) - Mouse ubiquitin-conjugating enzyme E2F (putative) (Ube2f), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Ube2f (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2F (putative) (Ube2f), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ube2f (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2F (putative) (Ube2f), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Ube2f (GFP-tagged ORF) - Rat ubiquitin-conjugating enzyme E2F (putative) (Ube2f), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ube2f (GFP-tagged ORF) - Rat ubiquitin-conjugating enzyme E2F (putative) (Ube2f), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Ube2f (Myc-DDK-tagged ORF) - Rat ubiquitin-conjugating enzyme E2F (putative) (Ube2f), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ube2f (Myc-DDK-tagged ORF) - Rat ubiquitin-conjugating enzyme E2F (putative) (Ube2f), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

18 Item(s)

per page
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