UBE2L3 / UBCH7 antibody

Principal name

UBE2L3 / UBCH7 antibody

Alternative names for UBE2L3 / UBCH7 antibody

Ubiquitin-protein ligase L3, Ubiquitin carrier protein L3, E2-F1, L-UBC, UBCE7, Ubiquitin-conjugating enzyme E2 L3

SwissProt ID

P68036 (Human), Q3MHP1 (Bovin)

Gene ID

7332 (UBE2L3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Dros (Drosophila)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of UBE2L3 / UBCH7 antibody

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro.This gene encodes a protein that has been identified as a component of NuRD, a nucleosome remodeling deacetylase complex identified in the nucleus of human cells. It shows a very broad expression pattern and is strongly expressed in many tissues. It may represent one member of a small gene family that encode different but related proteins involved either directly or indirectly in transcriptional regulation. Their indirect effects on transcriptional regulation may include chromatin remodeling. It is closely related to another member of this family, a protein that has been correlated with the metastatic potential of certain carcinomas. These two proteins are so closely related that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. One of the proteins known to be a target protein for this gene product is p53. Deacteylation of p53 is correlated with a loss of growth inhibition in transformed cells supporting a connection between these gene family members and metastasis.

General readings

Nuber U, Schwarz S, Kaiser P, Schneider R, Scheffner M. Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7 (E2-F1) and characterization of their interaction with E6-AP and RSP5. J Biol Chem. 1996 Feb 2;271(5):2795-800. PMID: 8576257

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UBE2L3 / UBCH7 antibody

  Rabbit Polyclonal antibody to UBE2L3 (ubiquitin-conjugating enzyme E2L 3)  
UBE2L3 antibody detects UBE2L3 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. 15 % SDS-PAGE. UBE2L3 antibody (TA308758) dilution: 1:1000 Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UBE2L3 / UBCH7 (C-term) antibody

  Goat Polyclonal Antibody against UBE2L3  
TA302697 staining (0.5µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

UBE2L3 / UBCH7 (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

UBE2L3 - human gene knockout kit via CRISPR

UBE2L3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

UBE2L3 - human gene knockout kit via CRISPR

UBE2L3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

UBE2L3 - human gene knockout kit via CRISPR

UBE2L3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

UBE2L3 - human gene knockout kit via CRISPR

UBE2L3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

UBE2L3 / UBCH7 (transcript variant 1)

  Recombinant protein of human ubiquitin-conjugating enzyme E2L 3 (UBE2L3), transcript variant 1  
UBE2L3 / UBCH7 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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