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UBE2M antibody

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Principal name

UBE2M antibody

Alternative names for UBE2M antibody

NEDD8-conjugating enzyme Ubc12, Ubiquitin-conjugating enzyme E2 M, NEDD8 protein ligase, NEDD8 carrier protein, UBC12

SwissProt ID

A3KN22 (Bovin), P61081 (Human), P61082 (Mouse)

Gene ID

9040 (UBE2M), 22192 (Ube2m)

Ncbi ID

9606, NP_003960.1, NP_003960, NP_663553.1, NM_001168469, NM_145578, NM_003969

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), African clawed frog, Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB)

Background of UBE2M antibody

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2M is a member of the E2 ubiquitin-conjugating enzyme family. It is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human UBE2M with an internal ID of P11237

General readings

Gong L., et al. (1999) J. Biol. Chem. 274:12036-12042Huang D.T., et al. (2004) Nat. Struct. Mol. Biol. 11:927-935

H00009040-M01 - UBE2M
Immunofluorescence of monoclonal antibody to UBE2M on HeLa cell. [antibody concentration 10 ug/ml]
H00009040-M01 - UBE2M
UBE2M monoclonal antibody (M01), clone 3C4 Western Blot analysis of UBE2M expression in Jurkat ( Cat # L017V1 ).
H00009040-M01 - UBE2M
Detection limit for recombinant GST tagged UBE2M is approximately 0.1ng/ml as a capture antibody.
ARP43030_P050 - UBE2M
Mouse Brain; . Sample Type: NT2 cells. Red: Antibody. Blue: DAPI. Primary Dilution: 1ug/50ul antibody. Secondary Antibody: Alexa goat anti-rabbit 594. Image Submitted by: Yuzhi Chen, University of Arkansas for Medical Sciences  .; UBE2M antibody - N-terminal region (ARP43030_P050) in Mouse Brain cells using Immunohistochemistry
ARP43030_P050 - UBE2M
Human brain stem; . Sample Type :. Human brain stem cells. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Goat anti-rabbit Alexa-Fluor 594. Secondary Antibody Dilution :. 1:1000. Color/Signal Descriptions :. UBE2M: Red DAPI:Blue. Gene Name :. UBE2M . Submitted by :. Dr. Yuzhi Chen, University of Arkansas for Medical Science; UBE2M antibody - N-terminal region (ARP43030_P050) in Human brain stem cells using Immunohistochemistry
ARP43030_P050 - UBE2M
Human 721_B; Host:Rabbit. Target Name:UBE2M. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. UBE2M is supported by BioGPS gene expression data to be expressed in 721_B.; UBE2M antibody - N-terminal region (ARP43030_P050) in Human 721_B cells using Western Blot
ARP43030_P050 - UBE2M
Mouse Brain; . . Sample Type. . :2. mouse brain extracts (80ug). Primary Antibody Dilution: 2ug/ml. Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience. Secondary Antibody Dilution: 1: 20,000. Image Submitted by: Yuzhi Chen. University of Arkansas for Medical Science .; UBE2M antibody - N-terminal region (ARP43030_P050) in Mouse Brain cells using Western Blot
ARP43030_P050 - UBE2M
Human 721_B; WB Suggested Anti-UBE2M Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysate. UBE2M is supported by BioGPS gene expression data to be expressed in 721_B.; UBE2M antibody - N-terminal region (ARP43030_P050) in Human 721_B cells using Western Blot
ARP43030_P050 - UBE2M
Human MCF7; Host:Rabbit. Target Name:UBE2M. Sample Tissue:Human MCF7. Antibody Dilution: 1.0ug/ml. UBE2M is supported by BioGPS gene expression data to be expressed in MCF7.; UBE2M antibody - N-terminal region (ARP43030_P050) in Human MCF7 cells using Western Blot
AR50427PU-N - UBE2M
TA503770 - UBE2M
Anti-UBE2M mouse monoclonal antibody (TA503770) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2M(RC208946).
TA503770 - UBE2M
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2M (RC208946, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2M.
TA503770 - UBE2M
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UBE2M monoclonal antibody.

3 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UBE2M antibody
  UBE2M mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)  
Anti-UBE2M mouse monoclonal antibody (TA503770) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2M(RC208946). Mouse IgG1 OTI2D9 Purified Can, Hu, Mky, Ms ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.
+2 additional images

TrueMAB

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

UBE2M (1-183, His-tag)
UBE2M Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  OriGene Technologies GmbH

UBE2M (1-183, His-tag)
UBE2M Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  OriGene Technologies GmbH

3 Item(s)

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Product Type
  1. Primary Antibody (1)
  2. Protein | Growth Factor (2)
Clonality
  1. Monoclonal (1)
Reactivity
  1. Canine (Can) (1)
  2. Human (Hu) (1)
  3. Monkey (Mky) (1)
  4. Mouse (Ms) (1)
Applications
  1. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  2. Western blot / Immunoblot (WB) (1)
Presentation
  1. Purified (3)
Host
  1. Mouse (1)
Images
  1. 1 image (3)
  2. 3+ images (1)
Images: Applications
  1. SDS-PAGE (2)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  3. Western blot / Immunoblot (WB) (1)
Species (Protein)
  1. Human (2)
Source
  1. E. coli (2)
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