UBE2N / BLU antibody

Principal name

UBE2N / BLU antibody

Alternative names for UBE2N / BLU antibody

Ubiquitin-conjugating enzyme E2 N, Ubiquitin-protein ligase N, Ubiquitin carrier protein N, UBC13, Bendless-like ubiquitin-conjugating enzyme

SwissProt ID

P61088 (Human), P61089 (Mouse), Q0P5K3 (Bovin), Q9EQX9 (Rat)

Gene ID

7334 (UBE2N), 93765 (Ube2n), 116725

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Dros (Drosophila), Ze (Zebrafish), African clawed frog, Bov (Bovine), Frog, Macaque, Mky (Monkey), Chimp (Chimpanzee), Xen (Xenopus), Eq (Equine), GP (Guinea Pig), Gt (Goat), Por (Porcine), Rb (Rabbit), Sh (Sheep), Ye (Yeast)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of UBE2N / BLU antibody

UBC13 is a member of the E2 ubiquitin-conjugating enzyme family also known as Ubiquitin-conjugating enzyme E2 N, Ubiquitin-protein ligase N, Ubiquitin carrier protein N and Bendless-like ubiquitin-conjugating enzyme. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. UBC13 forms a heterodimer with UBE2V2 which then catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. UBC13 also mediates transcriptional activation of target genes and plays a role in the control of progress through the cell cycle and differentiation, as well as error-free DNA repair. UBC13 also contributes to the survival of cells after DNA damage.

General readings

1. Chen, Z.J. (2005) Ubiquitin signalling in the NF-kappaB pathway. Nature Cell Biology. 7: 758-766.
2. Stagg, H.R. et al. (2009) The TRC8 E3 ligase ubiquitinates MHC class I molecules before dislocation from the ER.
J Cell Biol. 186: 685-92.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UBE2N / BLU antibody

  Rabbit Polyclonal Anti-UBE2N Antibody  
Sample Type: 1. Human NT-2 cells (60ug); 2. mouse brain extracts (80ug); Primary Antibody Dilution: 2ug/ml; Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience; Secondary Antibody Dilution: 1: 20, 000; Image Submitted by: Yuzhi Chen; U Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Sh, Ye, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

UBE2N / BLU antibody

  Rabbit Polyclonal UBC13 Antibody  
Western blot analysis of UBC13 in human small intestine cell lysates with UBC13 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

UBE2N / BLU (40-51) antibody

  Goat polyclonal anti-UBC13 antibody  
WB using Anti-UBC13 antibody shows detection of UBC13 protein in human small intestine lysate (lane 1) but not in mouse thymus lysate (lane 2). The heavily stained band in lane 1 (arrowhead) indicates this particular gel was overloaded with protein. The identity of minor reactive bands is unknown but could represent E2 complexes.The primary antibody was used at 1:500.Alexa FluorTM 680 conjugated Rb-a-Goat IgG was used at 1:10000. Goat IgG Aff - Purified Bov, Can, Chimp, Chk, Hu, Macaque, Ms, Rt, Xen, Ze E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

UBE2N / BLU antibody

  Rabbit Polyclonal Anti-UBE2N Antibody  
Lanes: 1: 40ng HIS-UBE2D1 protein; 2: 40ng HIS-UBE2D2 protein; 3: 40ng HIS-UBE2D3 protein; 4: 40ng HIS-UBE2D4 protein; 5: 40ng HIS-UBE2E1 protein; 6: 40ng HIS-UBE2E2 protein; 7: 40ng HIS-UBE2E3 protein; 8: 40ng HIS-UBE2K protein; 9: 40ng HIS-UBE2L3 protei Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Sh P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

UBE2N / BLU antibody

Western blot analysis of UBC13 in human small intestine cell lysates with UBC13 antibody at (A) 0.5, (B) 1, and (C) 2 µg/ml. Rabbit IgG Purified Hu, Ms, Rt C, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

UBE2N / BLU antibody

Skin: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

UBE2N / BLU antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cerebellum. Goat Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

UBE2N / BLU (C-term) antibody

Immunohistochemical staining of mouse thymus with Rabbit anti Mouse Ubc13 (SP2158P) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Ube2n - mouse gene knockout kit via CRISPR

Ube2n - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ube2n - mouse gene knockout kit via CRISPR

Ube2n - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ube2n - mouse gene knockout kit via CRISPR

Ube2n - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ube2n - mouse gene knockout kit via CRISPR

Ube2n - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

UBE2N / BLU

  Recombinant protein of human ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast) (UBE2N)  
UBE2N / BLU Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

UBE2N / BLU (1-152, His-tag)

UBE2N / BLU Human Purified > 95 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

UBE2N / BLU (1-152, His-tag)

UBE2N / BLU Human Purified > 95 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Ube2n (GFP-tagged) - Mouse ubiquitin-conjugating enzyme E2N (cDNA clone MGC:41464 IMAGE:1382079), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ube2n (GFP-tagged) - Mouse ubiquitin-conjugating enzyme E2N (cDNA clone MGC:41464 IMAGE:1382079), 200 uL, >10^7 TU/mL  
200 µl / €790.00
  OriGene Technologies, Inc.

Lenti ORF particles, Ube2n (GFP-tagged) - Mouse ubiquitin-conjugating enzyme E2N (Ube2n), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ube2n (GFP-tagged) - Mouse ubiquitin-conjugating enzyme E2N (Ube2n), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Ube2n (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2N (cDNA clone MGC:41464 IMAGE:1382079), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ube2n (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2N (cDNA clone MGC:41464 IMAGE:1382079), 200 uL, >10^7 TU/mL  
200 µl / €700.00
  OriGene Technologies, Inc.

Lenti ORF particles, Ube2n (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2N (Ube2n), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ube2n (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2N (Ube2n), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Ube2n (GFP-tagged ORF) - Rat ubiquitin-conjugating enzyme E2N (Ube2n), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ube2n (GFP-tagged ORF) - Rat ubiquitin-conjugating enzyme E2N (Ube2n), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

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