UBE2N / BLU antibody

Principal name

UBE2N / BLU antibody

Alternative names for UBE2N / BLU antibody

Ubiquitin-conjugating enzyme E2 N, Ubiquitin-protein ligase N, Ubiquitin carrier protein N, UBC13, Bendless-like ubiquitin-conjugating enzyme

SwissProt ID

P61088 (Human), P61089 (Mouse), Q0P5K3 (Bovin), Q9EQX9 (Rat)

Gene ID

7334 (UBE2N)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Dros (Drosophila), Ze (Zebrafish), African clawed frog, Fi (Fish), Xen (Xenopus), Bov (Bovine), Frog, Macaque, Mky (Monkey), Chimp (Chimpanzee), Prim (Primate), Eq (Equine), GP (Guinea Pig), Gt (Goat), Por (Porcine), Rb (Rabbit), Sh (Sheep), Ye (Yeast)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of UBE2N / BLU antibody

UBC13 is a member of the E2 ubiquitin-conjugating enzyme family also known as Ubiquitin-conjugating enzyme E2 N, Ubiquitin-protein ligase N, Ubiquitin carrier protein N and Bendless-like ubiquitin-conjugating enzyme. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. UBC13 forms a heterodimer with UBE2V2 which then catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. UBC13 also mediates transcriptional activation of target genes and plays a role in the control of progress through the cell cycle and differentiation, as well as error-free DNA repair. UBC13 also contributes to the survival of cells after DNA damage.

General readings

1. Chen, Z.J. (2005) Ubiquitin signalling in the NF-kappaB pathway. Nature Cell Biology. 7: 758-766.
2. Stagg, H.R. et al. (2009) The TRC8 E3 ligase ubiquitinates MHC class I molecules before dislocation from the ER.
J Cell Biol. 186: 685-92.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UBE2N / BLU antibody

Sample Type: 1. Human NT-2 cells (60ug); 2. mouse brain extracts (80ug); Primary Antibody Dilution: 2ug/ml; Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience; Secondary Antibody Dilution: 1: 20, 000; Image Submitted by: Yuzhi Chen; U Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Sh, Ye, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

UBE2N / BLU antibody

Mouse brainstem; . Sample Type :. Mouse brain stem cells. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Goat anti-rabbit Alexa-Fluor 594. Secondary Antibody Dilution :. 1:1000. Color/Signal Descriptions :. UBE2N: Red DAPI:Blue. Gene Name :. UBE2N. Submitted by :. Dr. Yuzhi Chen, University of Arkansas for Medical Science; UBE2N antibody - middle region (ARP33126_T100) in Mouse brainstem cells using Immunohistochemistry Rabbit Aff - Purified Can, Dros, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

UBE2N / BLU antibody

Western blot analysis of UBC13 in human small intestine cell lysates with UBC13 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

UBE2N / BLU (40-51) antibody

WB using Anti-UBC13 antibody shows detection of UBC13 protein in human small intestine lysate (lane 1) but not in mouse thymus lysate (lane 2). The heavily stained band in lane 1 (arrowhead) indicates this particular gel was overloaded with protein. The identity of minor reactive bands is unknown but could represent E2 complexes.The primary antibody was used at 1:500.Alexa FluorTM 680 conjugated Rb-a-Goat IgG was used at 1:10000. Goat IgG Aff - Purified Bov, Can, Chimp, Chk, Hu, Macaque, Ms, Rt, Xen, Ze E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

UBE2N / BLU antibody

Lanes: 1: 40ng HIS-UBE2D1 protein; 2: 40ng HIS-UBE2D2 protein; 3: 40ng HIS-UBE2D3 protein; 4: 40ng HIS-UBE2D4 protein; 5: 40ng HIS-UBE2E1 protein; 6: 40ng HIS-UBE2E2 protein; 7: 40ng HIS-UBE2E3 protein; 8: 40ng HIS-UBE2K protein; 9: 40ng HIS-UBE2L3 protei Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Sh P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

UBE2N / BLU antibody

Western blot analysis of UBC13 in human small intestine cell lysates with UBC13 antibody at (A) 0.5, (B) 1, and (C) 2 µg/ml. Rabbit IgG Purified Hu, Ms, Rt C, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

UBE2N / BLU antibody

Skin: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

UBE2N / BLU antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cerebellum. Goat Aff - Purified Bov, Can, Chk, Frog, Hu, Macaque, Mky, Ms, Rt, Ze E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

UBE2N / BLU control peptide

UBE2N / BLU Purified
50 µg / €190.00
  Acris Antibodies GmbH

UBE2N / BLU Control Peptide

UBE2N / BLU
50 µg / €160.00
  Acris Antibodies GmbH

UBE2N / BLU (C-term) antibody

Immunohistochemical staining of mouse thymus with Rabbit anti Mouse Ubc13 (SP2158P) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

UBE2N / BLU antibody

UBE2N / BLU Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

UBE2N / BLU antibody

UBE2N / BLU Mouse IgG1 4C11-G11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

UBE2N / BLU antibody

UBE2N monoclonal antibody (M02), clone 3G1-D10 Western Blot analysis of UBE2N expression in LNCaP ( Cat # L004V1 ). Mouse IgG2b 3G1-D10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

UBE2N / BLU antibody

Detection of UBE2N in human heart lysate with UBE2N polyclonal antibody ( Cat # PAB0191 ) at 2 µg/ml. Rabbit Hu IP, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

UBE2N / BLU antibody

Western blot analysis of UBE2N expression in human small intestine tissue with UBE2N polyclonal antibody at 1µg/ml. Rabbit Hu, Ms, Rt C, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

UBE2N / BLU antibody

Western blot using UBE2N polyclonal antibody ( Cat # PAB11286 ) shows detection of UBE2N protein inhuman small intestine lysate ( lane 1 ), but not in mouse thymus lysate ( lane 2 ).
The heavily stained band in lane 1 ( arrowhead ) indicates this particular gel was overloaded with protein.
The identity of minor reactive bands is unknown, but could represent E2 complexes.
Each lane contains approximately 20 µg of lysate.
Primary antibody was used at a 1 : 500 dilution.
The membrane was washed and reacted with a 1 : 10,000 dilution of Alexa FluorTM 680 conjugated Rb-a-Goat IgG. Goat Bov, Chimp, Chk, Frog, Hu, Macaque, Ms, Rt E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

UBE2N / BLU antibody

Human brainstem; . Sample Type :. Human brain stem cells. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Goat anti-rabbit Alexa-Fluor 594. Secondary Antibody Dilution :. 1:1000. Color/Signal Descriptions :. UBE2N: Red DAPI:Blue. Gene Name :. UBE2N. Submitted by :. Dr. Yuzhi Chen, University of Arkansas for Medical Science; UBE2N antibody - middle region (ARP34089_T100) in Human brainstem cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Can, Chk, Dros, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

UBE2N / BLU antibody

Detection of TRF4-1 in HeLa cell lysate with anti-TRF4-1 NB100-56357. Rabbit IgG Aff - Purified Hu WB
0.1 mg / €320.00
  Novus Biologicals Inc.

UBE2N / BLU antibody

UBE2N / BLU Goat Aff - Purified Bov, Can, Chk, Hu, Mky, Ms, Rt, Xen, Ze E, ICC/IF, P, WB
50 µg / €480.00
  Novus Biologicals Inc.

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