UBE3A antibody

Principal name

UBE3A antibody

Alternative names for UBE3A antibody

Ubiquitin-protein ligase E3A, E6AP, EPVE6AP, HPVE6A, NY-REN-54, Oncogenic protein-associated protein E6-AP, Human papillomavirus E6-associated protein

SwissProt ID

O08759 (Mouse), Q05086 (Human)

Gene ID

7337 (UBE3A)

Available reactivities

Hu (Human), Ms (Mouse), Xen (Xenopus), Bov (Bovine), Dros (Drosophila), Rt (Rat), Ze (Zebrafish), African clawed frog

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of UBE3A antibody

UBE3A interacts with the E6 protein of the cancer-associated human papillomavirus types 16 and 18. The E6/E6-AP complex binds to and targets the p53 tumor-suppressor protein for ubiquitin-mediated proteolysis. It is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It can target itself for ubiquitination in vitro and efficiently promotes its own degradation in vivo. It appears that only unmodified E6-AP molecules can bind efficiently to p53 in the presence of the HPV E6 oncoprotein. UBE3A binds UBQLN1 and UBQLN2. Defects in UBE3A are a cause of Angelman syndrome (AS) [MIM:105830]; also known as 'happy puppet syndrome'. AS is characterized by features of severe motor and intellectual retardation, microcephaly, ataxia, frequent jerky limb movements and flapping of the arms and hands, hypotonia, hyperactivity, hypopigmentation, seizures, absence of speech, frequent smiling and episodes of paroxysmal laughter, and an unusual facies characterized by macrostomia, a large mandible and open-mouthed expression, a great propensity for protruding the tongue ('tongue thrusting'), and an occipital groove. UBE3A contains 1 HECT-type E3 ubiquitin-protein ligase domain.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UBE3A antibody

Immunoperoxidase of monoclonal antibody to UBE3A on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml] Mouse IgG2a 2F6 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

UBE3A (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

UBE3A antibody

UBE3A Mouse IgG2a 3E5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

UBE3A antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBE3A polyclonal antibody ( Cat # PAB4222 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma . Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

UBE3A antibody

UBE3A Rabbit Hu, Ms IP, WB
0.1 ml / €400.00
  Abnova Taiwan Corp.

UBE3A antibody

Human HeLa; WB Suggested Anti-UBE3A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate. UBE3A is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells.; UBE3A antibody - N-terminal region (ARP42950_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Dros, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

UBE3A antibody

Human HeLa; . Lanes: Lane 1: 7ug HeLa lysate+EGFP SiRNA . Lane 2: 7ug HeLa lysate+UBE3A SiRNA. Primary Antibody Dilution: 1:300. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:500. Gene Name: UBE3A. Submitted by: Seiji Masuda, Kitashirakawa Oiwakecho, Kyoto University.; UBE3A antibody - middle region (ARP42951_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

UBE3A antibody

Western Blot: UBE3A Antibody [NB500-239]-Cytoplasmic (S100) fraction (10 mg) from HeLa cells. Affinity purified rabbit anti-E6AP  used at 20 mcg/10 mg extract. Rabbit Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

UBE3A antibody

Western Blot: UBE3A Antibody [NB500-240]-A. Tissue lysate from wildtype (wt) mice, E6AP knockout (KO) mice, or normal human (wt) tissue.  B. Cytoplasmic (S100) fraction (10 mg) from HeLa cells.  Affinity purified rabbit anti-E6AP used at 0.25 mcg/ml for WB and 20 mcg/10 mg lysate for IP. Rabbit Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

UBE3A antibody

Immunohistochemistry-Paraffin: UBE3A Antibody [NBP1-89092] - Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells and cells in molecular layer. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

UBE3A (transcript variant 3)

UBE3A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

UBE3A (transcript variant 2)

UBE3A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

UBE3A

UBE3A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

UBE3A

UBE3A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

UBE3A

UBE3A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

UBE3A

UBE3A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

16 Item(s)

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