Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)
Rabbit, Mouse, Goat
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C)
Background of Ubinuclein-1 (UBN1) antibody
Ubinuclein 1 (UBN1) is a ubiquitously expressed evolutionarily conserved protein which binds to proliferation-promoting genes that are repressed by formation of senescence-associated heterochromatin foci (SAHF). Ubinuclein 1 associates with various transcription factors and with histone methyltransferase activity, is indispensable for SAHF formation and appears to be a regulator of senescence. Although in most cells ubinuclein is localized to the nucleus, in cells forming tight junctions it is recruited to the cell adhesion complexes, dependently on the cell density.
Olsen,J.V., (2006) Cell 127 (3), 635-648
Human HeLa; WB Suggested Anti-UBN1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Hela cell lysate. UBN1 is supported by BioGPS gene expression data to be expressed in HeLa.; UBN1 antibody - C-terminal region (ARP47638_P050) in Human HeLa cells using Western Blot
Western blotting analysis of ubinuclein in nuclear fraction of HeLa cells. The monoclonal antibody UBN1-01 (lane A, B) detects ubinuclein 1 (165 kDa) and an 180 kDa protein - presumable ubinuclein 2. The antibody UBN1-02 (lane C, D) detects ubinuclein 1. Antibody dilution: A, C - 1 μg/ml; B, D - 5 μg/ml.
Western Blot: UBN1 Antibody [NBP1-49983] - Detection of Human Ubinuclein-1 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Ubinuclein-1 antibody used for WB at 0.1 mcg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western blotting analysis of ubinuclein in nuclear fraction of HeLa cells. The monoclonal antibody UBN1-01 (Lane A, B) detects ubinuclein 1 (165 kDa) and an 180 kDa protein - presumable ubinuclein 2. The antibody UBN1-02 (Lane C, D) detects ubinuclein 1. Antibody dilution: A, C - 1 µg/ml; B, D - 5 µg/ml.