ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Ubiquilin-like protein antibody
Transient overexpression lysate of ubiquilin-like (UBQLNL)
Western Blot analysis of UBQLNL expression in transfected 293T cell line (H00143630-T01) by UBQLNL MaxPab polyclonal antibody.Lane 1: MGC20470 transfected lysate(52.25 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: UBQLNL Antibody [NBP1-82737] - Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells along with distinctly stained inflammatory cells. .
Western Blot: UBQLNL Antibody [NBP1-82737] Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: Negative control (vector only transfected HEK293T lysate) | Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.