Ubiquitin antibody

Principal name

Ubiquitin antibody

Alternative names for Ubiquitin antibody

UBB, Polyubiquitin-B

SwissProt ID

P0CG47 (Human), P0CG49, P0CG51, P0CG53, P0CG55, P0CG62, Q8MKD1 (Horse)

Gene ID

7314 (UBB), 6233 (RPS27A)

Available reactivities

Hu (Human), Mam (Mammalia), Ms (Mouse), Rt (Rat), All Species, Bov (Bovine), Ce (C. elegans), Chk (Chicken), Dros (Drosophila), Pl (Plant), Broad, Br, Eq (Equine), Rb (Rabbit), Mky (Monkey), Por (Porcine), Arabidopsis, Oryza (Oryza sativa), Can (Canine), Hst (Hamster), Sh (Sheep), Xen (Xenopus), Ye (Yeast)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Frozen Sections (C), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunodiffusion (ID), Immunoprecipitation (IP)

Background of Ubiquitin antibody

Ubiquitin (Ub) is a small, 76-residue, protein (8.5 kDa) found both as free monomer and covalently attached to itself and other proteins in eukaryotic cells. Free Ub is a very compact and stable molecule that is easily refolded after being denatured. It is therefore recommended that for detection of free Ub on Westerns, the Tris-Tricine SDS-PAGE is used and nitrocellulose filters are autoclaved after the transfer and before blocking and addition of anti-Ub antibodies. The C-terminus of ubiquitin forms an isopeptide bond with the ε-amino group of a lysine side chain in a target protein. In this way proteins can be covalently modified by the addition of ubiquitin which may alter the target protein's function. Monoubiquitination generally targets proteins for internalization, endocytosis and lysosomal degradation, or modifies the surface charge of histones and affects chromatin compaction. Conjugation of ubiquitin (Ub) involves a three-step mechanism whereby specific enzymes (or enzyme complexes) activate and covalently link Ub to their substrates. Multi-ubiquitin chains can be built up on a single lysine of the target protein, by isopeptide bond formation usually between the carboxyl group of Gly76 of one Ub with the amino group of the side chain of Lys of the preceding ubiquitin. Although Lys48 of Ub is most frequently used for multi-ubiquitin chain formation, other lysines have also been shown to form bonds with Gly76. If a chain of multiple copies of ubiquitin is attached to a protein, this appears to target the protein for degradation by the large intacellular protease known as the 26S proteasome. The process of intracellular proteasomal proteolysis is very rapid and efficient. This makes detection of certain ubiquitinated proteins extremely difficult. Several specific inhibitors of proteasomes are commercially available and have been proven to be very useful in research applications. The detection of ubiquitinated species is further complicated by the fact that the process of ubiquitination is reversible and is accomplished by highly specific proteases present in all eukaryotic cells. It is therefore recommended that cell lysates are kept on ice and the lysis buffers contain one or more of cysteine protease inhibitors (n-ethylmaleimide, E64, etc.).

General readings

1. Ciechanover, A. (1998) EMBO J. 17, 7151-7160.
2. Hochstrasser, M. (2000) Nat. Cell Biol. 2, E153-E157.
3. Hochstrasser, M. (2000) Science 289, 563-564.
4. Bernardi, R. et al. (2000) Oncogene 19, 2447-2454.
5. Aberle, H. et al. (1997) EMBO J. 16, 3797-3804.
6. Salomoni, P. and Pandolfi, P.P. (2002) Nat. Cell Biol. 4, E152-E153.
7. Jesenberger, V. and Jentsch, S. (2002) Nat. Rev. Mol. Cell Biol. 3, 112-121.
8. Shelly, M. et al. (2011) Neuron 71, 433-46.
9. Yamada, K. et al. (2011) J Biol Chem 286, 44057-66.
Application References:

1. Magnaudeix A, et al. 2013. Neurobiol Aging. 34:770.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Ub antibody

Immunohistochemical analysis of Ub staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Mky, Ms, Por, Rb, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Ub antibody

Immunohistochemical analysis of Ub staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Mky, Ms, Por, Rb, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Ub antibody

Immunohistochemical analysis of Ub staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Mky, Ms, Por, Rb, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Ubiquitin+1 antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Ubiquitin+1 antibody

Ubiquitin+1 Mouse IgG2b Purified Hu WB
0.5 mg / €410.00
  Neuromics Antibodies

Ubiquitin+1 antibody

Ubiquitin+1 Rabbit Aff - Purified Hu C, P, WB
0.1 mg / €430.00
  Neuromics Antibodies

Ubiquitin antibody

Immunohistochemistry analysis of Ubiquitin Antibody Cat.-No AP06474PU-N in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Ubiquitin (N-term) antibody

Western Blot. 5 μg of recombinant protein from Phytophora sp. (Lane 1), human (Lane 2), Arabidopsis thaliana His-tagged ubiquitin (Lane 3), Arabidopsis thaliana His-tagged SUMO protein (Lane 4), was separated on 15% PAA gel and blotted on PVDF membrane. Filters were blocked in 5% milk for 1h, incubated with 1/10000 anti-Ubiquitin antibody (1h), followed by incubation with 1/15000 secondary anti-Rabbit antibodies (1h) coupled with HRP and visualization (10 seconds exposure) with standard ECL. Rabbit Serum Arabidopsis thaliana, Hu, Pl WB
0.1 ml / €430.00
  Acris Antibodies GmbH

Ubiquitin antibody

Western blot analysis using UBB antibody Cat.-No AM06722PU-N against human UBB (AA: 1-299) recombinant protein (Expected MW is 26 kDa). Mouse IgG1 3C12 Purified Hu E, F, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Ubiquitin (N-term) antibody

Immunohistochemistry: AP11229PU-N  Ubiquitin antibody staining of Formalin-Fixed, Paraffin-Embedded Human hepatocarcinoma tissue which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

Ubiquitin antibody

Immunohistochemistry: AP15846PU-N Ubiquitin antibody staining of Human Alzheimer's brain. Rabbit Purified Hu P
0.5 ml / €300.00
  Acris Antibodies GmbH

Ubiquitin antibody

Immunohistochemistry: AP15846PU-N Ubiquitin antibody staining of Human Alzheimer's brain. Rabbit Purified Hu P
1 ml / €410.00
  Acris Antibodies GmbH

Ubiquitin antibody

Immunohistochemistry: AP15846PU-N Ubiquitin antibody staining of Human Alzheimer's brain. Rabbit Purified Hu P
0.1 ml / €170.00
  Acris Antibodies GmbH

Ubiquitin antibody

IHC analysis of Ubiquitin using TA309498, tested on Bouin's fixed paraffin-embedded backskin sections of transgenic mice. Rabbit Purified Bov, Can, Chk, Dros, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh, Xen IP, WB
50 µl / €325.00
  OriGene Technologies, Inc.

Ubiquitin antibody

Western blot analysis of ubiquitinated proteins in HEK93 cells, using a dilution of anti-UBB. Rabbit Purified Bov, Can, Chk, Dros, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh, Xen IP, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Ubiquitin antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Ubiquitin antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Ubiquitin antibody

Anti-Ubiquitin antibody, generated by immunization with Ubiquitin coupled to Rabbit IgG, was tested by WB against total cell extract from yeast. Dilution of the antibody between 1:200 and 1:1,000 showed strong reactivity with Ubiquitinated proteins.  In this blot the antibody was used at 1:500. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG. Rabbit Purified Mam, Ye E, WB
0.5 mg / €325.00
  OriGene Technologies, Inc.

Ubiquitin antibody

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/25-50  The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using UBB antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Ubiquitin antibody

Western blot analysis of Ubiquitin in human cell lysates using a 1:1000 dilution of the antibody Mouse IgG2a 5B9-B3 Purified Bov, Hu, Ms, Rt E, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

Ubiquitin antibody

Western blot analysis of Ubiquitin in human cell lines using a 1:1000 dilution of the antibody Mouse IgG2a 6C11-B3 Purified Bov, Hu, Ms, Rt E, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

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